A kind of long-term preservation method of mycorrhizal fungus Pyriformis indica
A long-term preservation technology of Pyromorpha indica, which is applied in the field of microbial applications, can solve the problems of loss of plant root symbiosis, easy loss of vitality, and bacterial source pollution, so as to reduce the growth of miscellaneous bacteria, reduce the variation of bacterial source, and maintain the symbiotic ability Effect
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Embodiment 1
[0029] 1. Preservation medium preparation of Pyrocystis indica
[0030] For each liter of storage medium, add 1 / 2 MS basic medium, sucrose 12g / L, mannose 17g / L, agar powder 6g / L, distilled water to volume, boil, dilute hydrochloric acid to adjust the pH value to 5.8, and pack in 625 mL blue For vases, pack 10 bottles per liter of culture medium, seal with a ventilated cap, sterilize by autoclaving at 121°C for 20 minutes, and cool for later use.
[0031] 2. Preparation of sterile orchids
[0032] Under a sterile environment, inoculate sterile 3 cm seedlings of Oncidium with roots on the medium described in 1, place them at 25°C, 2000 lx, and 14 h / d light conditions, and cultivate them for 8 days for later use.
[0033] 3. Inoculation and storage
[0034] Open the oncidium culture bottle under aseptic conditions, and use a puncher to inoculate the vigorous bacterial mass around the ring of Pyromorpha indica cultured in PDA medium for a week to the root of the oncidium plant, ...
Embodiment 2
[0042] 1. Preservation medium preparation of Pyrocystis indica
[0043] For each liter of preservation medium, add 1 / 2 MS basic medium, sucrose 10g / L, mannose 15g / L, agar powder 5g / L, distilled water to volume, boil, dilute hydrochloric acid to adjust the pH value to 5.8, and pack in 625 mL blue For vases, pack 9 bottles per liter of culture medium, seal with a ventilated cap, sterilize by autoclaving at 121°C for 20 minutes, and cool for later use.
[0044] 2. Preparation of sterile orchids
[0045] In a sterile environment, inoculate the sterile clematis stems with roots into the storage medium, place them at 20° C., 2000 lx, and 14 h / d light conditions, and cultivate them for 7 days for later use.
[0046] 3. Inoculation and storage
[0047] Under aseptic conditions, open 2 described clematis culture bottles, and use a puncher to inoculate the vigorous bacterium block around the Pyromorpha indica bacterium ring that is newly cultivated in the PDA medium for one week to th...
Embodiment 3
[0055] 1. Preservation medium preparation of Pyrocystis indica
[0056] For each liter of storage medium, add 1 / 2 MS basic medium, sucrose 15g / L, mannose 20g / L, agar powder 6g / L, distilled water to volume, boil, dilute hydrochloric acid to adjust the pH value to 5.8, and pack in 625 mL blue For vases, pack 11 bottles per liter of culture medium, seal them with a 0.5 cm air-permeable film, sterilize them with high-pressure steam at 121°C for 20 minutes, and cool them down for later use.
[0057] 2. Preparation of sterile orchids
[0058] Under a sterile environment, inoculate sterile seedlings of Dendrobium candidum with roots in the preservation medium, place them at 28°C, 2000 lx, 14 h / d light conditions, and cultivate them for 10 days for later use.
[0059] 3. Inoculation and storage
[0060] Under aseptic conditions, open the Dendrobium officinale culture bottle described in 2, and use a puncher to inoculate the vigorous bacterium block around the Pyrosporium indica bact...
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