Immobilized microbe and preparation method and applications thereof

A technology for immobilizing microorganisms and microorganisms, applied in chemical instruments and methods, immobilized on/in organic carriers, applications, etc., can solve problems such as long fermentation time, insufficient water absorption and water retention, reagent pollution, etc. To achieve the effect of promoting vigorous reproduction, ensuring vigorous reproduction and good fixation

Active Publication Date: 2018-04-06
郭鹭超 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When organic matter such as rice husk is used as a fixed carrier, there are (1) insufficient water absorption and water retention; (2) insufficient gaps required for microbial fixation, resulting in poor aerobic and anaerobic balance in microbial reproduction, resulting in fermentation culture The time is too long; (3) The effect of composting or pollution treatment is poor
Immobilizing microorganisms through cross-linking reactions may lead to contamination of reagents, damage to microbial organisms, and accumulation of immobilized carriers that cannot be decomposed.

Method used

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  • Immobilized microbe and preparation method and applications thereof
  • Immobilized microbe and preparation method and applications thereof

Examples

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preparation example Construction

[0029] The embodiment of the present invention proposes a method for preparing immobilized microorganisms, which includes: immersing an organic immobilized carrier into a culture solution containing microorganisms, and obtaining immobilized microorganisms through fermentation and cultivation.

[0030] In this embodiment, the preparation step of the microorganism culture solution added to the organic immobilized carrier is to add the above-mentioned microorganisms to the nutrient source for cultivation. There is no special requirement for this cultivation method, and the usual method can be used. For example, first dissolve a certain amount of carbohydrates such as honey and syrup as a nutrient source in water at room temperature, add a certain amount of natto bacteria, yeast and lactic acid bacteria to it, and after mixing, slowly stir the mixture until it is about 35 ~ Under the condition of keeping warm at 40°C, the culture is carried out for 3 to 7 days, and the culture solu...

Embodiment 1

[0050] An immobilized microorganism provided in this embodiment is prepared according to the following steps:

[0051] If the moisture content is less than 60%, the apparent density is 0.2[g(dry) / cm 3 (wet)], the average pore diameter is 0.3mm, the maximum moisture content is 55 [g (water) / g (dry)] characteristics of dry carbonized cotton cloth, cut into samples of different regular shapes with the longest diameter of 30mm. It is used as the organic immobilized carrier that accounts for 100% by weight, according to the dosage ratio in Table 1, after microbial culture fluid and organic immobilized carrier are added in the airtight container, while exhausting, the temperature of container is controlled at At about 40°C, carry out fermentation culture, and obtain immobilized microorganisms with ethanol odor in about 3 days.

Embodiment 2

[0053] An immobilized microorganism provided in this example, the organic immobilized carrier used includes dry carbonized cotton cloth with a weight ratio of 60% and rice bran with a weight ratio of 40%, and is prepared according to the preparation steps of Example 1. immobilized microorganisms.

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Abstract

The invention provides an immobilized microbe and a preparation method and applications thereof, and relates to the technical field of sewage treatment. The immobilized microbe comprises an organic immobilizing carrier and microbes immobilized on the organic immobilizing carrier; and the organic immobilizing carrier comprises carbonized cotton cloth with a mass percentage of 5-100%. The inventionalso relates to a preparation method and applications of the immobilized microbe. Carbonized cotton cloth is added into the microbe immobilizing carrier, comprises gaps for immobilizing microbes, andpromotes the immobilization and strong reproduction of microbes. At the same time, carbonized cotton cloth has an enough performance on absorbing and maintaining water to guarantee the strong reproduction of microbes. moreover, carbonized cotton cloth has the advantages of easy degradation and low cost. The immobilized microbe can be used a fertilizer of compost, a soil improver, a fermentation starter/promoter, a deodorant, a wastewater cleanser, a bathing pool cleanser, a river/lake cleanser, and an air cleanser.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, and in particular to an immobilized microorganism, a preparation method and an application of the immobilized microorganism. Background technique [0002] With the development of the economy and the acceleration of the industrialization process, the increasingly serious pollution problem is becoming more and more prominent, especially the serious pollution of water bodies and soil. Scientists from all over the world are trying to find low-cost, low-energy pollution treatment technologies. Microorganisms have a unique role in removing pollutants, and biodegradation is regarded as a relatively effective, economical and energy-saving pollution treatment technology, especially in water treatment. When traditional microorganisms treat sewage, they usually directly inoculate the microorganisms in the sewage to be treated, and reduce the concentration of pollutants through the growth, absorpti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02C02F3/34B01D53/84C09K17/14
CPCB01D53/84C02F3/34C09K17/14C12N11/02Y02A50/20
Inventor 米川侑孝
Owner 郭鹭超
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