Cell preservation liquid

A technology of preservation solution and cells, applied in the field of cell preservation solution, can solve the problems such as the inability to meet large-scale preservation requirements and the degradation of cell activity, and achieve the effects of reducing ion pump activity, reducing the production of acidic substances, and strong buffering capacity.

Active Publication Date: 2018-04-20
SHANGHAI CRYOWISE MEDICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, under this storage condition, long-term storage of cells will

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  • Cell preservation liquid
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Embodiment Construction

[0036] Unless otherwise defined, the technical terms or scientific terms used in the claims and description of the present invention shall have the ordinary meanings understood by those skilled in the technical field to which the present invention belongs.

[0037] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the present invention will be further described below in conjunction with specific embodiments. It should be noted that the following embodiments are intended to facilitate the understanding of the present invention, and the present invention claims The scope is not limited in any way. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0038] In the following examples, unless otherwise specified, the methods used are conventional methods, and the...

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Abstract

The invention provides cell preservation liquid. The cell preservation liquid is prepared from the following components: 30 to 120mM of an osmotic pressure stabilizer, 2 to 6mM of a saccharide substance, 0 to 10mM of a reducing agent, 0 to 120mM of an electrolyte solution and 25 to 60mM of an amino acid; the osmotic pressure of the cell preservation liquid is 310 to 350mOsm/L. The cell preservation liquid provided by the invention can be used for effectively reducing cell metabolism and reducing low-temperature injuries under a low-temperature condition; living cells are convenient to preservein a transportation process, and functional active cells are also conveniently stored for a short period by an organization.

Description

technical field [0001] The invention belongs to the technical field of cell preservation, and in particular relates to a cell preservation solution and its application. Background technique [0002] Cells are the basic structural units of organisms, and the quality of cells will affect the state of the body. Cells are widely used in scientific research and clinical practice. The key to its application is the acquisition of a large number of donor cells and the overcoming of immune rejection. The acquisition of a large number of donor cells is mainly solved by in vitro cell culture and preservation. [0003] At present, there are still some problems in cell preservation, such as inconvenient transportation, inconvenient temporary storage, rapid decline in cell activity during transportation, and easy accumulation of cells into clumps. After the cells are transported for a long time, the viability of the cells is very low, and a large number of cells die, which directly affec...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 鄢和新杨秋蕊翟志敏吴红平唐为忠翟博
Owner SHANGHAI CRYOWISE MEDICAL TECH CO LTD
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