Capillary electrophoresis-based multi-dye-set nucleic acid analysis method and application thereof

Abstract

The invention relates to the field of medical diagnosis, in particular to a capillary electrophoresis-based multi-dye-set nucleic acid analysis method and application thereof. The method comprises thesteps of before electrophoresis is carried out, performing spectral correction respectively on a plurality of fluorescent dye sets to be used so as to obtain spectrum correction matrixes of all the dye sets, and setting a binning method of an image acquisition device according to the fluorescent dye sets adopted by all channel products; segmenting nucleic acid to be detected into fragment compositions containing a plurality of nucleic acid fragments, and labeling the fragment compositions with the plurality of fluorescent dye sets simultaneously or separately, and using a multi-channel capillary array electrophoresis apparatus to perform multi-channel electrophoresis detection on the labeled fragment compositions; obtaining the electrophoresis data of all channels by means of the image acquisition device, and carrying out spectrum unfolding on the electrophoresis data collected by all the channels by using the spectral correction matrixes. The capillary electrophoresis-based multi-dye-set nucleic acid analysis method can enable gene fragment analysis experiments to be more flexible, and reduces the time and cost of the gene fragment experiments.

Description

technical field [0001] The invention relates to the field of medical diagnosis, in particular to a capillary electrophoresis-based multi-dye set nucleic acid analysis method and its application. Background technique [0002] Capillary electrophoresis (capillary electrophoresis, CE) is a high-voltage electric field as the driving force for ions or charged particles, using capillary as the separation channel, and achieving efficient and rapid separation according to the difference in mobility or partition coefficient between components in the sample. A new type of electrophoresis technology. The instrument device includes a high-voltage power supply, a capillary tube, a detector, and two liquid storage bottles that are inserted into the two ends of the capillary tube and connected with the power supply. The separation process of capillary electrophoresis is a typical differential motion process. During the migration process of the mixture, each sample molecule will gradually...

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Problems solved by technology

[0004] However, in the prior art, in the DNA fragment analysis method, usually only one dye set can be used to mark the sample for each test. If there are many sites to be detected, it often requires multiple experiments, which affects the detection. aging

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