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Production process of probiotics colony additive for pig breeding feed

A production process and technology of probiotics, applied in animal feed, animal feed, microbial-based methods, etc., can solve problems such as drug resistance, drug residues, and harm to human health, so as to improve pig immunity, stable product quality, Good digestion and absorption

Inactive Publication Date: 2018-05-04
FOSHAN CHUANSUO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To treat animal diseases, chemical therapy is still widely used conventionally. The abuse of antibiotics has caused animal bacteria to develop drug resistance and drug residues, which indirectly endangers human health.

Method used

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  • Production process of probiotics colony additive for pig breeding feed
  • Production process of probiotics colony additive for pig breeding feed
  • Production process of probiotics colony additive for pig breeding feed

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0038] 1) Preparation of the first-level expanded culture medium for strains: put the first-level expanded culture medium for the strains into the fermenter, adjust the pH to 6.0-8.0, perform high-temperature disinfection, and use 0.5%- Add 3% of the mixed culture composed of Cordyceps militaris, Bacillus subtilis and yeast, and anaerobic culture for 24 hours at a temperature of 30°C-37°C and a stirring speed of 100-150 rpm. A primary expansion culture medium;

[0039] 2) Preparation of the secondary expansion culture medium of the strain: put the secondary expansion medium of the strain into the fermenter, adjust the pH to 6.0-8.0, carry out high-temperature disinfection, and press the volume of the secondary expansion medium of the strain to be 6%- 12% is added to the first-level expansion culture solution of the strains prepared in step 1), and the temperature is 30°C-37°C, and the stirring speed is 100-150 rpm, and the anaerobic culture is carried out for 36-48 hours, and ...

Embodiment 1

[0063] A production process for a probiotic colony additive for pig breeding feed, specifically comprising the following steps:

[0064] 1) Preparation of the first-level expansion culture medium of the strain: put the first-level expansion medium of the strain into the fermenter, adjust the pH to 6.0 with sodium hydroxide, sterilize at 121°C for 30 minutes, and cool to 30°C, According to the 0.5% of the volume of the first-level expansion medium of the strain, add a mixed culture composed of Cordyceps militaris, Bacillus subtilis and yeast according to the ratio of 1:1:1, at a temperature of 30 ° C, and a stirring speed of 100 rpm Under the condition of different points, anaerobic culture for 24 hours, the first-level expansion culture solution of the bacteria is formed;

[0065] The strain primary expansion medium is made of the following raw materials: 300ml of red sorghum concentrate, 300ml of rice bran concentrate, 300ml of wheat concentrate, 12L of potato concentrate, 30...

Embodiment 2

[0080] A production process for a probiotic colony additive for pig breeding feed, specifically comprising the following steps:

[0081] 1) Preparation of the first-level expansion culture medium of the strain: put the first-level expansion medium of the strain into the fermenter, and after all the raw materials in the medium are completely dissolved, adjust the pH to 7.0 with sodium hydroxide, and sterilize at 121°C For 30 minutes, when it was cooled to 35°C, add the culture composed of Cordyceps militaris, Bacillus subtilis and yeast according to 1:1:1 according to 2.5% of the volume of the first-level expansion medium of the strain, and at a temperature of 35 ℃, under the condition of stirring speed of 120 rpm, anaerobic culture for 24 hours, the first-level expansion culture solution of the strain;

[0082] The strain primary expansion medium is made of the following raw materials: 300ml of red sorghum concentrate, 300ml of rice bran concentrate, 300ml of wheat concentrate...

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Abstract

The invention discloses a production process of a probiotics colony additive for a pig breeding feed. The process comprises the following steps: 1) preparing a culture primary enlarge cultivation solution; 2) preparing a culture secondary enlarge cultivation solution; and 3) inoculating the culture and carrying out drying and production. The invention also provides an application of the probioticscolony additive in pig feeds. 100-300g of probiotics colony additive is added into every 50kg of the pig feed. The probiotics colony additive can help the establishment of intestinal tracts of the pigs and maintain dominant bacterial communities and promote the pigs to generate various biological active substances such as various digestive enzymes, vitamins, organic acids and somatomedin. The pigs are healthy in intestinal tract and good in digestive absorption naturally, and the weight gain is obvious.

Description

technical field [0001] The invention relates to a production process of a probiotic colony additive used for pig breeding feed, and belongs to the technical field of microbial feed additives. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the intestinal tract and reproductive system of humans or animals, can produce definite health effects, improve the host's micro-ecological balance, and exert beneficial effects. It works primarily by improving the balance of the host's gut microbiota. It has the functions of improving the structure of intestinal flora, inhibiting pathogenic bacteria, generating nutrients, improving the body's immunity, eliminating carcinogenic factors, lowering cholesterol and blood pressure, and improving lactose digestibility. Fuller first defined probiotics in 1989: "Probiotics are live bacterial additives that have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14A23K10/18A23K50/30C12R1/125C12R1/645
CPCA23K10/18A23K50/30C12N1/14C12N1/16C12N1/20
Inventor 梁健丰
Owner FOSHAN CHUANSUO BIOTECHNOLOGY CO LTD
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