A novel paenibacillus strain, antifungal compounds, and methods for their use

A technology of Bacillus and strains, applied in the field of Paenibacillus strains with broad-spectrum antifungal activity, can solve the problems of difficult development of active ingredients and high development costs

Active Publication Date: 2018-05-04
BAYER CROPSCI LP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, active ingredients with new mechanism

Method used

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  • A novel paenibacillus strain, antifungal compounds, and methods for their use
  • A novel paenibacillus strain, antifungal compounds, and methods for their use
  • A novel paenibacillus strain, antifungal compounds, and methods for their use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0172] Example 1. Selection of Paenibacillus sp. NRRL B-50972

[0173]The genomes of several Paenibacillus species strains were sequenced. Genomic data were analyzed to identify strains with the fusaridin biosynthesis gene cluster but lacking the polymyxin synthase gene cluster. Like the polymyxin synthase gene cluster, the gene cluster responsible for fusaridin biosynthesis (fusA) has been previously identified and characterized. See, e.g., Li et al., "Nonnbosomal Biosynthesis of Fusaricidins by Paenibacillus polymyxa PKB 1 Involves Direct Activation of a D-Amino Acid," Chemistry & Biology, 15:118-127 (2008); Li et al., "Promoter Analysis and Transcription Regulation of fus GeneCluster Responsible for Fusaricidin Synthesis of Paenibacillus polymyxa SQR-21,"Applied Microbiol Biotechnol,97:9479-9489 (2013); and Choi et al, "Identification of a Polymyxin Synthetase Gene Cluster of Paenibacillus polymyxa and Heterolibus in the Baceroloous Expression of "Journal of Bacteriology,...

Embodiment 2

[0175] Example 2. In-Plant Antifungal Activity of Paenibacillus Species Strain Whole Culture Solution

[0176] Selected Paenibacillus sp. strains, including Paenibacillus sp. strain NRRL B-50972, were grown in soybean-based media to produce whole broth cultures. Distilled water was added to each whole culture to make a final dilution of 10%.

[0177] Diluted whole broths were applied to leaves of young plants, which were subsequently exposed to fungal inoculums of tomato late blight (PHYTIN), botrytis cinerea (BOTRCI) or wheat leaf rust (PUCCRT). An untreated control was included in each assay for comparison. Several days after exposure to the fungal inoculum, each plant was scored for percent pathogen control relative to untreated control plants. Each treatment was evaluated three times, and the average control percentage of the whole culture solution of each Paenibacillus strain was as follows: figure 1 shown.

[0178] Of the 23 strains tested for antifungal activity aga...

Embodiment 3

[0179] Example 3. In Vitro Bioefficacy of Paenibacillus sp. Strain NRRL B-50972 Fusacidin Extract

[0180] Whole broth cultures of several Paenibacillus sp. strains, including Paenibacillus sp. strain NRRL B-50972, were prepared using soybean-based media. A lipophilic fraction containing fusaricidin was extracted from whole culture broth. Three separate fractions (i.e., Fraction 1, Fraction 2, Fraction 3) containing various Fusaridin and antifungal metabolites were prepared from whole broth extracts from Group 1 Bacillus sp. strains . Extracts from Paenibacillus sp. strain NRRL B-50972 were not further isolated.

[0181] Fusaricidin-containing fractions from each strain were tested against the following 12 fungal pathogens: Alternaria alternata (ALTEAL), Botrytis cinerea (BOTRCI), Fusarium spp. Phytophthora graminis (SEPPTR), Phytophthora graminis (PHYTCR), Phytophthora infestans (PHYTIN), Pythium ultima (PYTHUL), Magnaporthe grisea (PYRIOR), Rice sheath blight (RHIZSO ), ...

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Abstract

The present invention relates to a composition comprising a biologically pure culture of a fungicidal Paenibacillus sp. strain comprising a variant fusaricidin synthetase lacking a functional adenylation domain in the third module. The present invention also provides a composition comprising a biologically pure culture of a fungicidal Paenibacillus sp. strain or a cell-free extract thereof comprising at least one Paeniserine and at least one Paeniprolixin. Also provided are isolated compounds and methods of treating a plant to control a plant disease with the disclosed compositions and compounds.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Patent Application No. 62 / 138,765, filed March 26, 2015, and U.S. Provisional Patent Application No. 62 / 232,205, filed September 24, 2015, the contents of which are It is incorporated herein by reference in its entirety. [0003] References to Sequence Listings Submitted Electronically [0004] An official copy of the Sequence Listing is submitted electronically via EFS-Web as a Sequence Listing in ASCII format with the file name "BCS 159002WO_ST25.txt", created on March 21, 2016, 68 kilobytes in size, the Sequence Listing An official copy of the document shall be submitted together with the specification. The Sequence Listing contained in this file in ASCII format is part of the specification and is hereby incorporated by reference in its entirety. technical field [0005] The present invention relates to the field of bacterial strains and their ability to control ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01N47/44A01P3/00A01P1/00C07K7/06C12Q1/10C12R1/01
CPCA01N47/44C07K7/06C12N1/20C12N9/93C12Q1/10G01N2333/24A01N63/25C07K11/02C07K11/00C12Q1/02C07K7/54C12R2001/01C07K7/50C12N1/205C07K14/195C12N9/90
Inventor J·博D·M·约P·施温特克C·S·泰勒B·A·塔格
Owner BAYER CROPSCI LP
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