Application of SPAG7 (Sperm Associated Antigen) gene in preparing senile dementia diagnosis kit

A kind of Alzheimer's disease and gene technology, applied in the field of biomedicine, can solve the problems that need to be further verified, leave sequelae, and difficult to obtain the process, and achieve the effect of simplifying the quantitative detection process, strong selectivity, and good repeatability.

Active Publication Date: 2018-05-15
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] Existing methods for diagnosing disease progression include questionnaire-based MMSE scoring tests, neuronal imaging detection of Aβ amyloid, and some invasive methods such as analysis of relevant biomolecules (Aβ molecules, tau Proteins, etc.) to help in the diagnosis of AD. Studies have shown that AD biomarkers in cerebrospinal fluid can be used to diagnose AD with better accuracy, but there are two obvious disadvantages: the cost is too high, compared with peripheral blood-related biological The tissue acquisition process is difficult; the acquisition of cerebrospinal fluid causes great pain to the patient and may leave sequelae
In contrast, peripheral blood is a biological tissue that is relatively easy to obtain, and it is of great practical significance to study AD-related biological changes in peripheral blood. Some molecular markers related to Alzheimer's have been revealed in existing patents. For example, the YAP1 gene revealed by ZL2015104636167, the EAPP gene revealed by ZL2015104635287, and the 10 pathogenic genes related to Alzheimer's revealed by CN2016104739651, however, the above genes need further verification

Method used

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  • Application of SPAG7 (Sperm Associated Antigen) gene in preparing senile dementia diagnosis kit
  • Application of SPAG7 (Sperm Associated Antigen) gene in preparing senile dementia diagnosis kit

Examples

Experimental program
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Embodiment 1

[0029] Example 1 High-throughput sequencing and analysis

[0030] The samples came from Peking Union Medical College Hospital, and the informed consent of the subjects was obtained. Collect peripheral blood samples from 15 cases of senile dementia patients and 9 cases of healthy control peripheral blood samples for RNA extraction. After RNA extraction, agarose gel electrophoresis can be used to preliminarily determine whether the quality of the extracted RNA samples is qualified or not. for further transcriptome analysis. Then, the extraction of RNA samples was detected by NanoDrop1000 spectrophotometer, and the sample requirements for RNA-seq sequencing: OD260 / OD280 was 1.8-2.2.

[0031] The sequencing platform is the HiSeq 2500 high-throughput sequencing platform of Illumina, which performs high-throughput transcriptome deep sequencing. After sequencing, we use Fast-QC (http: / / www.bioinformatics.babraham.ac.uk / projects / fastqc / ) The software evaluates the quality of sequenc...

Embodiment 2

[0032] Example 2 SPAG7 gene expression in peripheral blood of senile dementia patients and healthy people

[0033] 1. Materials and methods

[0034] 1. Materials

[0035] The peripheral blood of 95 cases of senile dementia patients and 31 cases of healthy people were collected, grouped and numbered. 2. Method

[0036] 2.1 Extraction of total RNA from peripheral blood of senile dementia patients and peripheral blood of healthy people

[0037] use Reagent extracts sample RNA, and the experimental operation is carried out according to the product manual. For specific operations, see the manual.

[0038] Judgment criteria for RNA quality: the OD260 / OD280 value of the RNA sample is between 1.7 and 2.2; the electrophoretic pattern of total RNA has clear 28S and 18S bands; difference.

[0039] 2.2 Synthesis of cDNA by reverse transcription

[0040] use III Reverse Transcriptase (invitrogen, Cat. No. 18080-044) was used for cDNA reverse transcription, and the experimental op...

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Abstract

The invention relates to application of an SPAG7 (Sperm Associated Antigen) gene in preparing a senile dementia diagnosis kit, in particular to application of the SPAG7 gene and an expression productthereof in diagnosing and treating senile dementia diseases, and aims to solve the problem that senile dementia molecular markers are scarce at present. According to the application, peripheral bloodsamples of patients suffering from senile dementia and reference peripheral blood samples of healthy people are subjected to high-flux sequencing, the candidate gene SPAG7 is selected, an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) method shows that the SPAG7 gene has very good correlation with senile dementia, and a base is made for clinical gene diagnosis on senile dementia.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of the SPAG7 gene in the preparation of senile dementia diagnostic preparations, and more specifically to the application of the SPAG7 gene and its expression products in the diagnosis and treatment of senile dementia. Background technique [0002] Alzheimer's disease, also known as Alzheimer's (AD), the incidence rate of Alzheimer's disease in my country is 8 people per thousand people per year, and the incidence and prevalence of the disease after the age of 60 increase exponentially with age. Due to the emergence of my country's population aging problem, the increase in the number of elderly people makes this disease one of the important factors affecting the health and life of the elderly population in my country. However, there is still no effective treatment for Alzheimer's disease. Early diagnosis and treatment can help delay Therefore, it is very important to fin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68A61K45/00A61P25/28
CPCA61K45/00C12Q1/6883C12Q2600/158G01N33/6896G01N2800/2821
Inventor 肖枫汪冰怡向常娟
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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