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A kind of lactobacillus plantarum and its application, screening test method

A technology of Lactobacillus plantarum and microorganisms, which is applied in the field of microorganisms, can solve the problems of single species of lactic acid bacteria and cannot meet market demand, and achieve the effects of improving quality, reducing the production of ammoniacal nitrogen content, and reducing the content of acidic detergent fiber

Active Publication Date: 2019-01-29
CHENGDU URBAN MODERN AGRI IND TECH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Lactic acid bacteria is a silage microbial additive that is being promoted in recent years. Its main function is to adjust the composition of microorganisms in silage materials, quickly become the dominant flora, compete to inhibit the growth of harmful microorganisms and convert limited sugar, reduce the pH value of silage, and improve the quality of silage. Different silage materials have different fermentation characteristics, and the current lactic acid bacteria suitable for silage are single, which cannot meet market demand

Method used

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  • A kind of lactobacillus plantarum and its application, screening test method
  • A kind of lactobacillus plantarum and its application, screening test method
  • A kind of lactobacillus plantarum and its application, screening test method

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Embodiment 2

[0048] Physiological and biochemical assays: the physiological and biochemical characteristics of Lactobacillus plantarum in Example 1 were analyzed, and the results are shown in Table 2.

[0049] Different temperature test: the Lactobacillus plantarum in Example 1 was inoculated into the MRS liquid medium with an inoculum size of 3%, cultivated under the temperature conditions in Table 2, and its growth status was observed. The results are shown in Table 2.

[0050] Salt tolerance test: the plantarum lactobacillus in embodiment 1 is in the MRS liquid medium containing NaCl concentration 3.0% (w / v) and 6.5% (w / v), after cultivating at 30 ℃ for 2 days, measure its salt tolerance The results are shown in Table 2.

[0051] Acid-resistant test: after the Lactobacillus plantarum table in Example 1 was cultured at 30° C. for 7 days in the MRS liquid medium with the pH value in Table 2, its acid resistance was measured, and the results are shown in Table 2.

[0052] Sugar fermentati...

Embodiment 3

[0065] Plant Lactobacillus of the present invention is used as pasture silage additive

[0066] Forage grass with a height of 1.5-2m was collected from the Chongzhou base of Sichuan Agricultural University, cut into a length of about 20mm, and fresh samples were obtained. For each treatment, 300g of fresh samples were taken and put into plastic bags (25×35cm; Aodeju, China). Lactobacillus plantarum 1.0 * 10 of the present invention in example 1 8 Add 1ml of CFU / g fresh material to each bag of silage, then vacuum seal it, place it at room temperature to ferment, each treatment group has 3 repetitions, and open the bag after 60 days of silage to obtain silage samples.

[0067] 1. Sensory evaluation:

[0068] 1) Silage samples According to the sensory silage scoring standard and grade evaluation compiled by the German Farming Association (DLG) in 1899, sensory scores were performed on the smell, structure and color of the silage.

[0069] Table 4 Sensory Evaluation Criteria for...

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Abstract

The invention discloses a plant Lactobacillus and its application, screening and testing method. The plant Lactobacillus (Lactobacillus plantarum) is preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No. 15073; the plant milk The growth rate of the bacillus is fast and the acid production efficiency is high; the plantarum lactobacillus is used as a forage silage additive to improve the quality of the forage silage, and has wide application prospects.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum and its application and screening assay method. Background technique [0002] Silage is a storage method that converts the fermentation substrate in raw materials into acidic substances such as lactic acid through the proliferation of lactic acid bacteria, and maintains an acidic anaerobic environment to facilitate the long-term preservation of silage crops. Silage is yellow-green in color, sour in smell, soft and juicy, and has good palatability. It is widely used in animal husbandry production, especially in ruminant breeding, and has become an important source of high-quality roughage. [0003] Lactic acid bacteria is a silage microbial additive that is being promoted in recent years. Its main function is to adjust the composition of microorganisms in silage materials, quickly become the dominant flora, compete to inhibit the growth of harmful ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C12Q1/689C12Q1/04A23K30/18C12R1/25
CPCA23K30/18C12N1/02C12N1/20C12Q1/689C12R2001/25C12N1/205A23V2400/169
Inventor 张新全关皓闫艳红帅杨李小铃李小梅黄琳凯聂刚汪霞黄婷
Owner CHENGDU URBAN MODERN AGRI IND TECH RES INST CO LTD