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Multiplex pcr detection method for genes related to skin traits

A trait and gene technology, applied in the field of genetic testing, can solve the problems of high cost and high sequence dependence when the number of loci is small

Active Publication Date: 2021-05-25
美因健康科技(北京)有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the cost is high when the number of sites is small, and the dependence on the sequence near the site is high

Method used

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  • Multiplex pcr detection method for genes related to skin traits
  • Multiplex pcr detection method for genes related to skin traits
  • Multiplex pcr detection method for genes related to skin traits

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Embodiment Construction

[0059] The present invention is explained in detail below.

[0060] Multiplex PCR Primer Design

[0061] Through the comparison of various factors, within an acceptable cost range, multiplex PCR is more suitable for the detection of genes related to skin characteristics at nearly a hundred sites. The design of primers for multiplex PCR is the key and difficult point, which is directly related to the cost and effect of detection. A set of primers with better effect was designed after several experiments, and the primer sequences are shown in Table 1. The selection principle of the primer combinations in Table 1 is designed based on the experience of previous experiments, and then the primers with poor effects are redesigned according to the experimental results. Under this condition, each SNP can reach the specified sequencing depth. If it is a common primer combination, under the specified average sequencing depth, some SNP sites cannot be detected or the sequencing depth i...

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Abstract

The invention provides a multiplex PCR detection method for genes related to skin traits, which comprises the following steps: (1) designing a primer combination; (2) purifying the product by magnetic beads after the first PCR amplification; (3) combining step (2) The purified product is subjected to the second PCR amplification, and the amplified product is subjected to magnetic bead purification; (4) The product purified in step (3) is subjected to next-generation sequencing. The method of the invention solves the problem of the amplification effect of these sites related to skin characteristics detected by multiplex PCR technology, and can significantly reduce the detection cost and increase the detection effect.

Description

technical field [0001] The invention relates to gene detection, in particular to a multiplex PCR detection method for genes related to skin traits. Background technique [0002] With the improvement of people's living standards, the demand for skin care products is increasing. In addition to the influence of external factors such as living environment and care level, genes have an important impact on the characteristics of human skin, and even directly determine the characteristics of skin. There are single base mutations (SNP) in some important positions of genes. Different genotypes will be formed after a base mutation at a certain site, and different genotypes often determine different skin characteristics. By detecting your own specific genotype, you can more effectively choose skin care products according to your own characteristics, so that you can perform skin care in a more targeted manner. [0003] There are currently a variety of techniques for the detection of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/686C12Q1/6869
CPCC12Q1/686C12Q1/6869C12Q1/6888C12Q2600/156C12Q2537/143C12Q2535/122
Inventor 戚珠云肖哲焦少灼禹黎尹朝华
Owner 美因健康科技(北京)有限公司