Identification method of relevant SSR (Simple Sequence Repeats) mark sites for early-mature molecular breeding of upland cotton

Abstract

The invention discloses an identification method of relevant SSR (Simple Sequence Repeats) mark sites for early-mature molecular breeding of upland cotton. The identification method comprises the following steps: by using a middle-late mature cotton variety cotton Lumianyan No.37 as a female parent and using an early-mature cotton variety Lumianyan No.19 as a male parent, hybridizing and selfing afirst generation to obtain F2; selecting 1 / 8 of early-mature plant lines and 1 / 8 of late-mature plant lines in an F2 group; planting F3 and selfing to obtain F4; performing identification at a firsttime on early-mature relevant SSR mark sites by utilizing the F2 group; finally performing chi-squared analysis and preliminarily determining characters of seedling and bud stages to select effectiverelevant SSR mark sites for early-mature molecular breeding of the upland cotton; performing identification for a second time on the early-mature relevant SSR mark sites by utilizing an F4 group, finally performing chi-squared analysis and finally determining the characters of the seedling and bud stages to select effective relevant SSR mark sites for early-mature molecular breeding of the uplandcotton. The method has the beneficial effect that the support of the reliable molecular mark sites is provided for the early-mature molecular breeding of the upland cotton.

Description

technical field [0001] The invention belongs to the technical field of crop breeding, and relates to a method for identifying SSR marker sites related to early-maturing molecular breeding of upland cotton. Background technique [0002] Cotton precociousness is an excellent comprehensive trait, mainly including traits such as full growth period, seedling stage, bud stage, flowering and boll stage, first fruiting branch position and pre-frost flowering rate, but these traits are quantitative traits and are affected by multiple quantitative traits. The control of genetic loci for traits has complex genetic mechanisms and is easily affected by environmental factors; traditional breeding methods mainly rely on the experience of breeders, and it is difficult to accurately grasp the traits related to precocity, so traditional breeding methods lack predictability and low efficiency. [0003] Cotton precociousness is a quantitative trait. Traditional genetic analysis methods are diff...

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Problems solved by technology

[0002] Cotton precociousness is an excellent comprehensive trait, mainly including traits such as full growth period, seedling stage, bud stage, flowering and boll stage, first fruiting branch position and pre-frost flowering rate, but these traits are quantitative traits and are affected by multiple quantitative traits. The control of trait gene loci, the genetic mechanism is complex and easily affected by environmental factors; traditional breeding methods mainly rely on the experience of breeders, and it is difficult to accurately grasp the traits related to precocity, so traditional breeding methods lack predictability and low efficiency

[0003] Cotton precociousness is a quantitative trait. Traditional genetic analysis methods are difficult to accurately track and locate the flow direction of the target gene in the hybrid offspring. The development of molecular marker technology provides an effective tool for the study of quantitative traits.

Linkage analysis and association analysis are the main methods to study the genotypes of plant quantitative traits at present. Using linkage analysis, the growth stage, seedling stage, bud stage, flowering and boll stage, first fruiting branch position, first fruiting branch height and pre-frost flowering have been located. QTL (Li C, Wang X, Na D, et al. QTL analysis for early-maturing traits in cotton using two upland cotton (Gossypium hirsutum L.) crosses[J]. Breeding Science, 2013, 63(2):154-163.), but the QTL mapping parent is not a production variety with a large promotion area in production, and the QTL mapping analysis cannot detect the alleles that exist but have no difference in the mapping population parents , and the located QTL has a strong "population specificity", so the research results of QTL mapping analysis based on linkage analysis are difficult to be directly applied to the genetic improvement of early maturity traits in cotton varieties

Linkage analysis has been used to obtain three SSR loci that are significantly associated with early maturity-related traits of upland cotton, among which CER0098-400 is significantly associated with the whole growth period, DPL0375-250 and HAU2414-147 are significantly associated with the initial node position of fruiting branches (Liang Bing et al. , Association Analysis of Upland Cotton Agronomic Traits and SSR (Simple Sequence Repeats) Markers, Acta Cotton Sinica, 26(5): 387-395), however, no molecular markers significantly associated with cotton precociousness-related traits obtained by using The loci guide detailed reports on cotton early-maturity breeding, because the early-maturity-related molecular marker loci of upland cotton obtained by association analysis have not been verified whether they are closely linked to cotton early-maturity target traits, and the target traits associated with these molecular markers are in early-maturity Breeding aspects not conducive to guiding assisted selection

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