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A urine microalbumin/urine creatinine detection kit

A urine microalbumin and detection kit technology, which is applied in the direction of chemiluminescence/bioluminescence, and analysis by making materials undergo chemical reactions, can solve the problems of cumbersome ACR measurement and inaccurate measurement results, and achieve linear width and measurement Precise, measured results

Active Publication Date: 2018-12-11
GUANGZHOU JINDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a urine microalbumin / urine creatinine detection kit to solve the problems of cumbersome ACR measurement and inaccurate measurement results

Method used

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  • A urine microalbumin/urine creatinine detection kit
  • A urine microalbumin/urine creatinine detection kit
  • A urine microalbumin/urine creatinine detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] As an embodiment of the urine microalbumin / urine creatinine detection kit of the present invention, the urine microalbumin / urine creatinine detection kit of this embodiment is:

[0054] Immobilized rabbit anti-human serum albumin polyclonal antibody: rabbit anti-human serum albumin polyclonal antibody coated on the luminescent plate;

[0055] Reagent R1 includes components at the following concentrations: HEPES buffer 0.02mol / L (pH 7.0), creatinase 20KU / L, sarcosine oxidase 10KU / L, trehalose 2wt%, Tween 20 0.003wt% , catalase 1.0KU / L, bilirubin oxidase 2.0KU / L, vitamin C oxidase 10KU / mL, DMSO0.5wt% and Proclin300 0.045wt%;

[0056] Reagent R2 includes the following concentrations of components: TAPS buffer 0.05mol / L (pH 8.0), creatinase 30KU / L, NaN 3 0.006wt%, Tween 80 0.008wt%, trehalose 1.0wt% and Proclin3000.045wt%;

[0057] Luminescence substrate 1: 1,2-dioxetane boronic acid;

[0058] Reagent R3 comprises the following concentration components: PBS buffer 0.01m...

Embodiment 2

[0062] As an embodiment of the urine creatinine / urine microalbumin detection kit of the present invention, the urine microalbumin / urine creatinine detection kit of this embodiment is:

[0063] Immobilized rabbit anti-human serum albumin polyclonal antibody: rabbit anti-human serum albumin polyclonal antibody coated on the luminescent plate;

[0064] Reagent R1 includes the following concentrations of components: Tris-HCl buffer 0.01mol / L (pH 6.0), creatinase 10KU / L, sarcosine oxidase 5KU / L, trehalose 2wt%, dodecyl -β-D maltoside 0.005wt%, catalase 0.1KU / L, bilirubin oxidase 3KU / L, vitamin C oxidase 5KU / mL, DMSO 0.5wt% and Proclin3000.2wt%;

[0065] Reagent R2 comprises the following concentration components: TAPS buffer 0.05mol / L (pH is 8.0), creatinase 10KU / L, dodecyl-β-D maltoside 0.005wt%, trehalose 2wt% and Proclin300 0.2 wt%;

[0066] Luminescence substrate 1: 1,2-dioxetane boronic acid;

[0067] Reagent R3 comprises the following concentration components: PBS buffer s...

Embodiment 3

[0071] As an embodiment of the urine microalbumin / urine creatinine detection kit of the present invention, the urine microalbumin / urine creatinine detection kit of this embodiment is:

[0072] Immobilized rabbit anti-human serum albumin polyclonal antibody: rabbit anti-human serum albumin polyclonal antibody coated on the luminescent plate;

[0073] Reagent R1 includes the following concentrations of components: PBS buffer 0.1mol / L (pH 7.5), creatinase 20KU / L, sarcosine oxidase 8KU / L, trehalose 0.5wt%, dodecyl- β-D maltoside 0.005wt%, bilirubin oxidase 1KU / L, vitamin C oxidase 5KU / mL, DMSO 2wt% and Proclin300 0.045wt%;

[0074] Reagent R2 comprises the components of the following concentrations: PBS buffer 0.1mol / L (pH is 7.5), creatinase 30KU / L, dodecyl-β-D maltoside 0.005wt%, trehalose 0.1wt% and Proclin300 0.045wt%;

[0075] Luminescence substrate 1: 1,2-dioxetane boronic acid;

[0076] Reagent R3 comprises the following concentration components: phosphate buffer 0.01mol...

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Abstract

The invention discloses a urine microalbumin / urine creatinine detection kit. The kit comprises a urine creatinine detection reagent and a urine microalbumin detection reagent, wherein the urine creatinine detection reagent comprises a reagent R1 used for eliminating endogenous creatine and sarcosine, a reagent R2 used for converting creatinine into creatine and a luminescent substrate 1 used for detecting hydrogen peroxide; the urine microalbumin detection reagent comprises an immobilized rabbit-anti-human serum albumin polyclonal antibody, a reagent R3 used for binding urine microalbumin andthe immobilized rabbit-anti-human serum albumin polyclonal antibody, an alkaline phosphatase labelled reagent R4 used for binding with urine microalbumin and a luminescent substrate 2 used for detecting alkaline phosphatase. Urine microalbumin and urine creatinine are detected with a glow-type chemiluminiscence method; the detection method can detect two indexes on a chemiluminescence instrument and enables measurement to be more accurate, more convenient and rapider.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a urine microalbumin / urine creatinine detection kit. Background technique [0002] ACR (urine microalbumin / urine creatinine ratio) plays an important role in judging the early diagnosis of diabetic nephropathy. There are two main methods to detect this indicator: one is through the urine analysis test card of urinary microalbumin / urine creatinine To measure the ACR value, the second is to measure the values ​​of urine microalbumin and urine creatinine respectively, and then obtain their values ​​by calculation. Urinary microalbumin measurement methods include enzyme-linked immunoassay, radioimmunoassay, immunoturbidimetry, colloidal gold method, transmission turbidimetry, and immunoturbidimetry; creatinine detection methods mainly include dry chemical and enzymatic methods. [0003] The shortcomings of existing technologies and measurement methods for the de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 齐宗献李民友丘传添林伟荣罗宁
Owner GUANGZHOU JINDE BIOTECH
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