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A kind of cell culture plate with polymer fiber and its preparation method

A cell culture and polymer technology, which is applied in the field of cell engineering, can solve the problem of unstable cell culture plate orientation arrangement pattern, etc., and achieve the effect of effectively guiding cell orientation arrangement and good orientation arrangement pattern.

Inactive Publication Date: 2020-06-09
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing a cell culture plate with polymer fibers, which solves the problem of unstable alignment patterns on the cell culture plate

Method used

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  • A kind of cell culture plate with polymer fiber and its preparation method
  • A kind of cell culture plate with polymer fiber and its preparation method
  • A kind of cell culture plate with polymer fiber and its preparation method

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Embodiment Construction

[0033] In the in vitro cell culture technology, in order to obtain aligned cells, many methods have been invented, such as mechanical load stimulation, surface chemical treatment and patterning.

[0034] Mechanical load stimulation can simulate the mechanical factors of the in vivo environment, stretch, compress, and shear cells, and can effectively guide the orientation and arrangement of cells, but it is difficult to be directly applied in vitro.

[0035] Surface chemical treatment, use traditional photolithography to draw patterns, and then fix integrin proteins on the patterns. Cells are affected by integrin proteins and patterns, and finally achieve orientation alignment, but this method lacks flexibility and reliability depends on Adhesion of integrin proteins to motifs therefore limits the application of this method.

[0036] Patterning can provide a special three-dimensional structure, comparable to the structure of natural extracellular matrix in vivo, and thus has gr...

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Abstract

The invention discloses a method for preparing a cell culture plate with polymer fibers. The method comprises the following steps: heating a polymer to a molten state; extruding the polymer in a molten state from a nozzle; forming polymer fibers on a conductive collecting plate at a distance of 1.5mm to 5.0mm from the nozzle; controlling the conductive collecting plate to move according to a preset trajectory, and forming a plurality of mutually parallel polymer fibers on the conductive collecting plate, thereby forming a cell culture substrate with the polymer fibers on the conductive collecting plate. The cell culture plate prepared by the method provided by the invention has a very good alignment pattern and has a good alignment effect on cells, thereby realizing the effective guidanceof cell alignment in in vitro cell culture. The invention also provides a cell culture plate with polymer fibers, which has the beneficial effects.

Description

technical field [0001] The invention relates to the technical field of cell engineering, in particular to a preparation method of a cell culture plate with polymer fibers and a cell culture plate with polymer fibers. Background technique [0002] Cell orientation exists in many tissues and organs in the human body, such as ligaments, tendons, striated muscles, blood vessels, nerve tissues, bone tissues, etc. Cell lines with oriented tissues and their surrounding extracellular matrix can exert special biological functions. Therefore, aligned cells play an indispensable role in the field of in vitro cell culture. [0003] In order to obtain aligned cells, many methods have been invented, such as mechanical loading stimulation, surface chemical treatment and patterning. However, the obtained aligned cells have various problems. At present, electrospinning can produce continuous nanofibers simply and quickly, so it can effectively prepare alignment patterns and effectively gu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D04H1/728D04H1/435D01D5/00C12M3/00
CPCC12M25/06D01D5/003D01D5/0092D04H1/435D04H1/728
Inventor 梁烽汤亚东蓝兴梓周颖曾俊杜志云王晗陈新度
Owner GUANGDONG UNIV OF TECH
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