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Method for identifying Jinhua pig and landrace pig on basis of SNP (single nucleotide polymorphism) sites

A Landrace pig, site technology, applied in the field of animal molecular biotechnology and animal genetic breeding

Inactive Publication Date: 2018-06-08
ZHANGZHOU AONONG MODERN AGRI DEV CO LTD +3
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there are few SNP marker combinations for variety identification and individual identification

Method used

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  • Method for identifying Jinhua pig and landrace pig on basis of SNP (single nucleotide polymorphism) sites
  • Method for identifying Jinhua pig and landrace pig on basis of SNP (single nucleotide polymorphism) sites
  • Method for identifying Jinhua pig and landrace pig on basis of SNP (single nucleotide polymorphism) sites

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Embodiment Construction

[0023] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0024] The present invention uses Jinhua pigs and Landrace pigs as research materials, uses Illumina 60K SNP chips to carry out genome-wide genotyping of these pig individuals, analyzes and screens out as few SNP marker combinations as possible that can be used for identification of Jinhua pigs and Landrace pigs.

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Abstract

The invention relates to a method for identifying Jinhua pig and landrace pig on the basis of SNP (single nucleotide polymorphism) sites. The differences of the SNP sites on Jinhua pig and landrace pig SSC(Sus scrofa chromosome)1 are used for identifying the Jinhua pig and the landrace pig; the SNP names are respectively MARC0039661, ASGA0001510, INRA0001043, DRGA0000202, MARC0041365, DRGA0000334,INRA0003745, INRA0003746, INRA0004022, CASI0011475, INRA0004282, DRGA0001542, DRGA0001542, INRA0004305, INRA0004312, ALGA0006239, INRA0004460, MARC0046211, MARC0036323 and M1GA0001181. The total number of the SNP sites is 20. Compared with the prior art, the method has the advantages that through the selection of 20 SNP sites, the Jinhua pig and the landrace pig can be cheaply, fast and conveniently identified through the testing on the SNP sites.

Description

technical field [0001] The invention relates to the fields of animal molecular biotechnology and animal genetic breeding, in particular to a method for identifying Jinhua pigs and Landrace pigs based on SNP sites. Background technique [0002] China is one of the earliest countries in the world to raise pigs, with a history of thousands of years. Pigs are known as the first of the six animals in China. Due to the influence of natural environment, economic conditions and folk culture, China's local pig breeds are rich in resources and have their own characteristics, which are valuable resources in the world's pig breed gene pool. But so far, there are few SNP marker combinations for variety identification and individual identification. [0003] With the application of high-density SNP chips in pigs, the popularization and development of whole genome sequencing technology, and the continuous decline in genotyping and sequencing costs, it is possible to establish individual tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/156
Inventor 龙毅黄立林王书影万文峰郭永平吴有林
Owner ZHANGZHOU AONONG MODERN AGRI DEV CO LTD
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