Hydrolytically degradable poss-peg hybrid hydrogel and its preparation method and use

A hydrogel, PEG-SH technology, applied in biochemical equipment and methods, pharmaceutical formulations, cell encapsulation, etc., can solve the problems of reducing the activity of biological cells, limiting the application of POSS hybrid hydrogels, etc., to improve survival ability, non-toxic hydrolysis, good biocompatibility

Active Publication Date: 2021-04-16
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the hydrophobicity of POSS, the POSS hybrid hydrogels currently studied are in organic solvents (such as toluene, tetrahydrofuran (THF), dimethyl sulfoxide (DMSO), etc.) or mixed solvents (such as acetone and water Mixed solvents) for gel formation, and organic solvents will reduce the activity of biological cells embedded in 3D hydrogels, which greatly limits the application of POSS hybrid hydrogels in biomaterials

Method used

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  • Hydrolytically degradable poss-peg hybrid hydrogel and its preparation method and use
  • Hydrolytically degradable poss-peg hybrid hydrogel and its preparation method and use
  • Hydrolytically degradable poss-peg hybrid hydrogel and its preparation method and use

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Experimental program
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Effect test

Embodiment 1

[0043] Preparation of POSS-PEG hybrid hydrogel

[0044]Maleimide-based cross-linked hydrolyzable POSS-PEG hybrid hydrogels were prepared by the following method: 4-arm-PEG-MAL (Mn = 20KDa, Beijing Jiankai Technology Co., Ltd.) and POSS-SH (provided by Sigma-Adrich Company) was dissolved in 3 mL of tetrahydrofuran, stirred at room temperature for 3.5 h, and then the solvent was evaporated to obtain a POSS-PEG prepolymer, which was stored at -20°C for future use. Then the POSS-PEG prepolymer and PEG-SH (Mn=4KDa) were uniformly mixed in different concentrations of triethanolamine buffer (TEA, pH=7.4) to obtain a pregel solution. Then the above pregel solution was transferred to five 600 μl molds, and reacted in a 37° C. biochemical incubator to prepare a hydrogel. The amounts of 4-arm-PEG-MAL, POSS-SH and hydrolyzable cross-linking agent, the molecular weight of 4-arm-PEG-MAL, the concentration of triethanolamine and the gelation time during the preparation process are shown in ...

Embodiment 2

[0055] Mechanical performance testing experiment

[0056] A set of hydrogels consisted of three test samples, which were swelled to equilibrium with ultrapure water, and the surface moisture was wiped dry with filter paper. The mechanical properties of the hydrogel were analyzed by rheometer, and the average value of the three test samples was taken. Figure 5 It is the oscillation stress scanning spectrum of the storage modulus (G') and the dynamic frequency scanning spectrum of the storage modulus (G') and loss modulus (G") of the hydrogel material. By Figure 5 It can be seen that when the hydrogel material prepared by the method of the present invention is subjected to an increase from 0.1Pa to 10Pa, the storage modulus (G') does not change significantly with the change of stress, and when the scanning frequency range is 0Hz to 10Hz There was also no significant change in the storage modulus (G'), indicating that the hydrogel material was well cross-linked. Compared with ...

Embodiment 3

[0058] Swelling rate test experiment

[0059] A set of hydrogels consisted of 3 test samples, which were freeze-dried and weighed accurately. The dry hydrogel was immersed in 0.9% sodium chloride injection (10 mL), and placed in a constant temperature incubator at 37°C. At a predetermined time, take out the swollen hydrogel, dry the surface moisture with filter paper and weigh until the weight of the hydrogel remains constant. Swelling percentage of hydrogel (%)=(hydrogel mass after swelling−hydrogel mass before swelling) / hydrogel mass before swelling×100%, average value of 3 test samples. Image 6 is the swelling curve. Depend on Image 6 It can be seen that the hydrogel material prepared by the method of the present invention has reached a higher swelling ratio in the first 24 hours, and reached swelling equilibrium after 100 hours. Although the equilibrium swelling ratio of POSS-PEG hybrid hydrogels decreased with the increase of POSS content. However, the equilibrium ...

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Abstract

The invention belongs to the field of hydrogel polymer materials, and in particular relates to a hydrolytically degradable POSS-PEG hybrid hydrogel and a preparation method and application thereof. The method comprises steps: 1) 4‑arm‑PEG‑MAL and POSS‑SH obtain POSS‑PEG prepolymer through Michael addition reaction in a solvent; 2) dissolve POSS‑PEG prepolymer and PEG‑SH in weak A pregel solution is obtained in an alkaline buffer, and the pregel solution is polymerized by Michael addition crosslinking at a reaction temperature to obtain a hydrolytically degradable POSS‑PEG hybrid hydrogel. The hydrogel obtained by the invention has fast gelation speed, good mechanical strength, good biocompatibility, non-toxicity, and can be hydrolyzed and degraded, and is a tissue engineering scaffold material with development potential.

Description

technical field [0001] The invention belongs to the field of hydrogel polymer materials, and in particular relates to a hydrolytically degradable POSS-PEG hybrid hydrogel and a preparation method and application thereof. Background technique [0002] Polyethylene glycol (PEG) hydrogel is a type of polymer that can absorb and retain a large amount of water. PEG has the characteristics of good biocompatibility, non-toxicity, and low immunogenicity. It can be excreted through the kidneys. Approved by the FDA, it is a very attractive potential tissue engineering scaffold and drug delivery material. Most of the PEG hydrogels reported so far are obtained by Michael addition polymerization or free radical polymerization. The main disadvantage of free radical-initiated polymerization is that free-radical crosslinking can greatly reduce the viability of encapsulated cells in vivo, and in situ crosslinked hydrogels are also inconvenient for transport in vivo. In contrast, Michael ad...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G81/00A61L27/18A61L27/52A61L27/58C12N5/00C12N5/071
CPCA61L27/18A61L27/52A61L27/58A61L2400/12C08G81/00C12N5/0012C12N5/069C12N2533/30C08L71/02
Inventor 卢翠芬李鹤玲沈金瑞陈祖兴杨桂春聂俊琦王飞翼
Owner HUBEI UNIV
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