Application of Campylobacter brevis abundance in tongue coating as a biomarker for determining the risk of gastritis-carcinoma transformation and related determination system
A biomarker and campylobacter technology, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of patient injury, adverse patient health, and difficulty in long-term adherence to sampling
Active Publication Date: 2021-02-05
TSINGHUA UNIV
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Problems solved by technology
In this long process, it is difficult to persist in sampling for a long time
Secondly, sampling from tissue has certain damage to the patient, and continuous sampling is not conducive to the health of the patient
Method used
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Embodiment
[0044] 1. Experimental design and sample collection.
[0045] Ninety-three gastritis patients collected from the hospital were selected for inclusion. For all these 93 patients, the tongue coating was photographed with a tongue imager, the tongue coating samples were scraped, and the patient's gender, age, pathological results, and TCM phenotype were recorded. At the same time, 50 healthy people were selected as normal controls in Tsinghua University. Tongue coating images were taken, tongue coating samples were collected, and information such as age and sex were recorded.
[0046] Extract microbial DNA from the tongue coating samples of the 93 gastritis patients and 50 healthy individuals obtained above, and determine the content of Campylobacter brevis in each sample. The specific operations are:
[0047] 1. Use the above kit to detect the content of Campylobacter brevis in the tongue coating:
[0048] a. Scraping of tongue coating:
[0049] a1. Prepare three 1.5ml EP tu...
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The present invention provides an application of a reagent for detecting the abundance of a non-destructive biomarker in the preparation of a composition for determining the risk of gastritis-cancer transformation, wherein the biomarker is Campylobacter brevis in the patient's tongue coating. Also provided are a detection kit for determining the risk of gastritis cancer transformation and a system for determining the risk of gastritis cancer transformation. The gastritis cancer transformation risk assay system is used to determine the abundance of a biomarker related to gastritis cancer transformation, including: the kit, used to determine the quantitative detection results of Campylobacter brevis on the tongue coating; the abundance of Campylobacter brevis on the tongue coating The determination part is used to determine the abundance of Campylobacter brevis in the tongue coating according to the quantitative detection result.
Description
technical field [0001] The present invention relates to the application of a reagent for detecting the abundance of a biomarker, wherein the biomarker is Campylobacter brevis in an extract of the patient's tongue coating, in the preparation of a composition for the determination of gastritis cancer transformation risk; the present invention also relates to A detection kit for measuring the risk of gastritis cancer transformation, a system for measuring the risk of gastritis cancer transformation, and a method for measuring the risk index of gastritis cancer transformation. Background technique [0002] Epidemiological data in recent decades and more and more clinical evidences show that "uncontrollable inflammation" is closely related to tumorigenesis [1]. At present, at least 15% of malignant tumors in the world, especially those in the digestive system, respiratory system, and genitourinary system, are caused by uncontrollable inflammation [2]. For example, the occurrence...
Claims
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IPC IPC(8): C12Q1/6886C12Q1/06C12R1/01
CPCC12Q1/6851C12Q1/689C12Q2521/107
Inventor 李梢张学工崔佳星崔鸿飞杨明冉
Owner TSINGHUA UNIV