A combined detection microfluidic chip for early diagnosis of kidney injury and its preparation method and application

Abstract

The invention relates to a combined detecting micro-fluidic chip for early diagnosis of renal injury. The chip comprises a chip substrate and an upper cover piece covering the chip substrate, whereina sample adding region, an antibody coating region, a micro mixing region, a first detection region, a second detection region, a quality control region and a waste liquor collecting region communicating through capillary micro channels successively are arranged on the chip substrate; a sample adding hole, a first detection window, a second detection window, a quality control window and a vent hole are formed in the upper cover piece, and the sample adding region, the first detection region, the second detection region, the quality control region and the waste liquor collecting region are corresponding in position successively. The invention also provides a preparation method and an application of the combined detecting micro-fluidic chip for early diagnosis. The combined detecting micro-fluidic chip for early diagnosis can achieve combined detection of items of neutrophile granulocyte gelatinase and microalbumin, and has extremely great clinical diagnostic value in early diagnosis ofrenal injury fully and precisely.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis, and in particular relates to a combined detection microfluidic chip for early diagnosis of kidney injury and its preparation method and application. Background technique [0002] Neutrophil gelatinase-associated lipocalin (NGAL) has a molecular weight of about 25kDa, covalently binds to neutrophil gelatinase, and is mainly involved in the occurrence and growth of early kidney epithelium. The expression of NGAL is low in normal tissues including kidney, lung, stomach and colon epithelial tissues. In the process of ischemic and toxic renal injury, NGAL in renal tubular epithelial cells will increase significantly, and within the first two hours, the level of NGAL in blood and urine will increase significantly, so NGAL as a reflection of renal function damage The markers have significant clinical significance. It is suitable for the detection of chronic kidney disease (CKD), diabetic nephropathy,...

AI Technical Summary

Problems solved by technology

[0004] With the continuous deepening of research, the test cards of NGAL and MAU items are gradually on the market. However, the current clinical diagnosis of renal function is mainly based on the detection of a single item, which leads to certain limitations in the diagnosis of diseases.

Or in order to obtain accurate test results, combined testing of kits of different items is used clinically, which requires a large amount of samples, a long detection cycle, a large workload, and low efficiency.

Images