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A kind of SNP marker related to chicken carcass traits and its application, detection primer, detection kit

A carcass trait and marking technology, applied in the field of biological breeding, can solve the problems of difficult 12-week-old body weight, carcass-causing mutation, and large confidence interval, and achieve the effect of accurate and reliable detection results and strong operability.

Active Publication Date: 2021-09-07
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the confidence intervals of these quantitative trait loci in most reports are relatively large, and it is difficult to identify the causative mutations affecting 12-week-old body weight and carcass traits through the map

Method used

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  • A kind of SNP marker related to chicken carcass traits and its application, detection primer, detection kit
  • A kind of SNP marker related to chicken carcass traits and its application, detection primer, detection kit
  • A kind of SNP marker related to chicken carcass traits and its application, detection primer, detection kit

Examples

Experimental program
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Effect test

Embodiment 1

[0027] The SNP markers related to chicken carcass traits in this example are shown in SEQ ID NO.1, the 5' end of the sequence is G or A at position 427, and this SNP site corresponds to the chicken reference genome Gallus_gallus-5.0 version published by NCBI Sequence information Deoxynucleotide at position 350006 on the sense strand of chromosome 16.

[0028] In the comparison of the transcriptome data of chicken liver tissue at 20 weeks and 30 weeks, it was found that NONGGAT003016 was significantly differentially expressed, and it was speculated that this gene may be related to the growth and development of chickens. Analysis of the gene sequence found that there is a SNP site, that is, as shown in SEQ ID NO.1, the 427th position from the 5' end of the sequence is G or A, so the correlation analysis is used to study the effect of this site on chicken growth and development. effects on traits.

Embodiment 2

[0063] The detection primers used to detect chicken carcass traits in the present embodiment are as follows:

[0064] SNP-F: 5'-AGGGAGCCTTGTTCAGTTGG-3';

[0065] SNP-R: 5'-AGCAAGGAAATCCACAGCGA-3'.

Embodiment 3

[0067] The detection kit used for detecting chicken carcass traits in this example includes the primers shown in Example 2, and also includes dNTPs, PCR reaction buffer, DNA polymerase and SauI endonuclease.

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Abstract

The invention relates to a SNP marker related to chicken carcass traits and its application, a detection primer and a detection kit, belonging to the technical field of biological breeding. The present invention discovers for the first time the G>A mutation site at position 427 at the 5' end of the sequence shown in SEQ ID NO.1, and the SNP site corresponds to Chromosome 16 of the Chicken Reference Genome Gallus_gallus‑5.0 version sequence information published by NCBI The 350006th deoxynucleotide of the sense strand. The body weight and meat production performance of AA homozygous chicken individuals in this SNP marker are significantly higher than those of GG and GA genotype individuals, which can be used for the selection of chicken carcass traits, and can be used for early selection of chicken carcass traits , to speed up the chicken breeding process and save production costs.

Description

technical field [0001] The invention relates to a SNP marker related to chicken carcass traits and its application, a detection primer and a detection kit, belonging to the technical field of biological breeding. Background technique [0002] In poultry production, chicken carcass traits are important economic traits, which can reflect chicken growth rate and meat production performance. Therefore, research on chicken carcass traits has important economic value. [0003] Under the same feeding environment conditions, the main reason for the differences in body weight and carcass traits at 12 weeks of age among individual chickens was genetic factors. At present, a total of 41 quantitative trait loci distributed on 18 chromosomes were found to be associated with 12-week-old body weight, and 67 quantitative trait loci distributed on 22 chromosomes were associated with carcass weight. However, the confidence intervals of these quantitative trait loci in most reports are relati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李红刘小军顾真真王彦彬田亚东李国喜韩瑞丽闫峰宾李转见蒋瑞瑞孙桂荣康相涛
Owner HENAN AGRICULTURAL UNIVERSITY