Unlock instant, AI-driven research and patent intelligence for your innovation.

SNP (Single Nucleotide Polymorphism) marker related to chicken carcass traits, application of SNP marker, detection primer and detection kit

A technology for carcass traits and detection primers, which is applied in the determination/inspection of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., can solve problems such as large confidence intervals, difficult 12-week-old weight and carcass mutations, etc. Achieve strong operability, accurate and reliable test results

Active Publication Date: 2018-07-06
HENAN AGRICULTURAL UNIVERSITY
View PDF4 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the confidence intervals of these quantitative trait loci in most reports are relatively large, and it is difficult to identify the causative mutations affecting 12-week-old body weight and carcass traits through the map

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SNP (Single Nucleotide Polymorphism) marker related to chicken carcass traits, application of SNP marker, detection primer and detection kit
  • SNP (Single Nucleotide Polymorphism) marker related to chicken carcass traits, application of SNP marker, detection primer and detection kit
  • SNP (Single Nucleotide Polymorphism) marker related to chicken carcass traits, application of SNP marker, detection primer and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The SNP markers related to chicken carcass traits in this example are shown in SEQ ID NO.1, the 5' end of the sequence is G or A at position 427, and this SNP site corresponds to the chicken reference genome Gallus_gallus-5.0 version published by NCBI Sequence information Deoxynucleotide at position 350006 on the sense strand of chromosome 16.

[0028] In the comparison of the transcriptome data of chicken liver tissue at 20 weeks and 30 weeks, it was found that NONGGAT003016 was significantly differentially expressed, and it was speculated that this gene may be related to the growth and development of chickens. Analysis of the gene sequence found that there is a SNP site, that is, as shown in SEQ ID NO.1, the 427th position from the 5' end of the sequence is G or A, so the correlation analysis is used to study the effect of this site on chicken growth and development. effects on traits.

Embodiment 2

[0063] The detection primers used to detect chicken carcass traits in the present embodiment are as follows:

[0064] SNP-F: 5'-AGGGAGCCTTGTTCAGTTGG-3';

[0065] SNP-R: 5'-AGCAAGGAAATCCACAGCGA-3'.

Embodiment 3

[0067] The detection kit used for detecting chicken carcass traits in this example includes the primers shown in Example 2, and also includes dNTPs, PCR reaction buffer, DNA polymerase and SauI endonuclease.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an SNP (Single Nucleotide Polymorphism) marker related to chicken carcass traits, application of the SNP marker, a detection primer and a detection kit, and belongs to the technical field of biological breeding. A G larger than A mutation site in a 427th site starting from a 5' terminal in a sequence as shown in SEQ ID NO. 1 is firstly discovered, and the SNP site corresponds to 350,006th deoxynucleotide of a positive-sense chain of a chromosome XVI in serial information published in a chicken reference genome Gallus_gallus-5.0 version by NCBI (National Center of Biotechnology Information). In the SNP marker disclosed by the invention, the weight and the meat performance of AA homozygous type individual chicken in a late growth stage are remarkably higher than theweight and the meat performance of GG and GA genotype individuals, the SNP marker is used for breeding the chicken carcass traits, the chicken carcass traits can be selected in an early stage, a breeding process of chicken is quickened, and the production cost is reduced.

Description

technical field [0001] The invention relates to a SNP marker related to chicken carcass traits and its application, a detection primer and a detection kit, belonging to the technical field of biological breeding. Background technique [0002] In poultry production, chicken carcass traits are important economic traits, which can reflect chicken growth rate and meat production performance. Therefore, research on chicken carcass traits has important economic value. [0003] Under the same feeding environment conditions, the main reason for the differences in body weight and carcass traits at 12 weeks of age among individual chickens was genetic factors. At present, a total of 41 quantitative trait loci distributed on 18 chromosomes were found to be associated with 12-week-old body weight, and 67 quantitative trait loci distributed on 22 chromosomes were associated with carcass weight. However, the confidence intervals of these quantitative trait loci in most reports are relati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李红刘小军顾真真王彦彬田亚东李国喜韩瑞丽闫峰宾李转见蒋瑞瑞孙桂荣康相涛
Owner HENAN AGRICULTURAL UNIVERSITY