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A kind of dyeing method and application of filamentous microorganism

A technology of filamentous microorganisms and dyeing methods, applied in the acquisition/recognition of microscopic objects, preparation of test samples, fluorescence/phosphorescence, etc., can solve the problems of high production cost, long time required, and impossibility of real application

Active Publication Date: 2021-06-22
LAIR LIQUIDE SA POUR LETUDE & LEXPLOITATION DES PROCEDES GEORGES CLAUDE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, these existing methods still have many deficiencies, such as traditional proliferation culture, biochemical test, serological test, although the accuracy is good, the required time is long
Although gene chips and protein chips using molecular biology technology are accurate, have a large throughput, and can be quantified, their production costs are too high to be truly applied to large-scale industrial sewage treatment.

Method used

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  • A kind of dyeing method and application of filamentous microorganism
  • A kind of dyeing method and application of filamentous microorganism
  • A kind of dyeing method and application of filamentous microorganism

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0196] 1.1 Preparation of sample staining solution

[0197] Staining solution A (Syto 9) and B (PI staining solution) were taken and prepared respectively with ultrapure water so that the final concentrations reached 3.34mM and 20mM. Store away from light, ready to use now.

[0198] Under the condition of 20x objective lens, the corresponding area of ​​field of view is 630μm×630μm, 396900μm 2 , adjust the field of view area on this basis, and select the appropriate objective lens and field of view according to the characteristics of the sample, that is, the samples in the target area can be clearly identified, there is almost no interference between the target samples, and the relative number is greater than 3 and clearly visible.

[0199] 1.2 Sample pretreatment

[0200] 1.2.1 Pre-judgment and preliminary determination of sample usage

[0201] After the target sample is collected or acquired, it is necessary to pre-judge the usage amount each time. The steps are as follows...

Embodiment 1

[0271] On January 1, 2016, 100mL of sewage from the outlet and inlet of the Haibohe Sewage Treatment Plant (Qingdao Haibohe Sewage Treatment Plant, No. 8, Hangzhou Branch Road, Shibei District, Qingdao) was collected in sterilized glass bottles, and the Back to the lab for testing.

[0272] 1. Mortality detection

[0273] After sampling, the samples were placed in a 4°C sampling box and immediately sent to the laboratory for processing. After the target sample is collected or obtained, it is necessary to pre-judge the usage amount each time. The steps are as follows:

[0274] After the sample is obtained, first shake it well, then take 10 μL of the sample in a sterile environment to make a glass slide, and observe with an ordinary light microscope to determine whether the target object is evenly dispersed and whether the concentration is appropriate. If there is no obvious overlapping image in the field of view, it is judged that the concentration is appropriate and no dilut...

Embodiment 2

[0289] On January 2, 2016, 100mL of sewage from the outlet and inlet of the Haibohe sewage treatment plant was taken in a sterilized glass bottle, and brought back to the laboratory for detection according to the same steps as in Example 1.

[0290] Confocal laser observation was performed on 10 μL of the stained sample. 60 fields of view were randomly selected, photographed, and used for mortality software analysis. The exit mortality rate was 35.82%, and the entrance mortality rate was 9.15%. The specific results are as follows:

[0291]

[0292]

[0293] 2. Length detection of filamentous microorganisms

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Abstract

The invention relates to a method for staining and identifying microorganisms. The invention provides a dyeing solution for filamentous microorganisms, a dyeing method, a fluorescence identification method, and a rapid evaluation method for the abundance of filamentous microorganisms. The present invention also provides a method for identifying the killing effect of filamentous microorganisms, and its use in testing the operation capacity and efficacy of water treatment equipment.

Description

technical field [0001] The present invention relates to methods for staining and identifying microorganisms, as well as staining solutions used therein, image processing methods, and computer-readable media. Background technique [0002] With the rapid development of urbanization and industry, the global sewage volume has also increased rapidly, causing the current sewage treatment equipment to seriously exceed the load. In the current sewage treatment, especially in the process of urban sewage treatment, the killing effect of microorganisms that can degrade organic matter, transform nitrogen, phosphorus, etc., especially the killing effect of bacteria is very important. However, how to effectively evaluate the operating capacity and efficacy of existing water treatment equipment, especially the killing effect of microorganisms, there is no unified method. At present, the analysis of microbial flora in sewage is mainly through the determination of chemical indicators (rapid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30G01N21/64G06K9/00
CPCG01N1/30G01N21/64G01N2001/302G06V20/69
Inventor 明磊强张大海杨宗霖史雅琦
Owner LAIR LIQUIDE SA POUR LETUDE & LEXPLOITATION DES PROCEDES GEORGES CLAUDE
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