A preparation method of a hydrogel chip with a biconical circular lumen structure

A hydrogel and biconical technology, applied in the field of tissue engineering and biomedicine, can solve the problems of single material, cell toxicity, and need to be improved, and achieve the effect of promoting cell growth, facilitating cell attachment, and less cell damage

Active Publication Date: 2020-04-03
ENERGY RES INST OF SHANDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The template method usually uses a circular tube of uniform diameter as a template, such as Chinese patent CN201110215024.5, which is prepared by cross-linking and solidifying the hydrogel solution by ultraviolet irradiation in the presence of a photoinitiator polymer, wherein the photoinitiator Polymers are easy to cause toxicity to cells; and this patent only uses polymethyl methacrylate material mold, which is a single material; in addition, the microchannel template used in this patent is a stainless steel capillary or fiber, which is a circular tube with a uniform diameter. Modification, only normal blood vessel models with uniform diameter can be produced, and pathological blood vessel shapes such as arterial thrombus cannot be simulated
[0005] The known preparation methods of blood vessel models, the cell culture and differentiation process are relatively complicated, the permeability and precision of blood vessels need to be improved, and the tubular structure with uniform diameter is usually prepared, which is difficult to effectively simulate the human pathological blood vessel structure

Method used

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  • A preparation method of a hydrogel chip with a biconical circular lumen structure
  • A preparation method of a hydrogel chip with a biconical circular lumen structure
  • A preparation method of a hydrogel chip with a biconical circular lumen structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Use a glass capillary with a diameter of 300 μm to stretch the template to form a biconical channel template with a maximum diameter of 300 μm and a minimum diameter of 100 μm; use a polystyrene square box frame to fix the template; use gelatin, collagen and phosphoric acid The mixture of buffer solutions is poured, wherein the mass percentage concentration of gelatin is 12.5%, and the mass percentage concentration of collagen is 0.1%. After the gelatin is solidified, the glass capillary template is removed to form a hydrogel chip with a biconical circular lumen structure, and the inlet and outlet can be connected with a silicone tube through a connecting needle. Human umbilical vein endothelial cells were digested and diluted to a concentration of 5×10 6 Cells / ml cell suspension is poured into the chip through a syringe through a silicone tube, and placed in a cell culture incubator for standing to adhere to the wall. After adhering to the wall, turn the hydrogel chip...

Embodiment 2

[0077] According to the method of Example 1, the difference is that a glass capillary with a diameter of 500 μm is stretched to make a template to form a biconical channel template with a maximum diameter of 500 μm and a minimum diameter of 122 μm.

Embodiment 3

[0079] According to the method of Example 1, the difference is that a glass capillary with a diameter of 700 μm is stretched to make a template to form a biconical channel template with a maximum diameter of 700 μm and a minimum diameter of 259 μm.

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Abstract

The invention relates to a hydrogel chip with a double cone circular tube cavity structure, a preparation method thereof and a method for preparing a pathological blood vessel model by using the hydrogel chip. Hydrogel is prepared by using a hydrogel solution and the like as raw materials through a template method, and the chip can truly simulate a human pathological vascular environment, and provide a novel pathological vascular model in vitro for basic researches and drug researches.

Description

Technical field: [0001] The invention belongs to the fields of tissue engineering and biomedicine, and relates to a hydrogel chip with a biconical circular lumen structure and a preparation method thereof, and a method for preparing a pathological blood vessel model by using the hydrogel chip. Background technique: [0002] Blood vessels are important organs in the human body, spreading all over the body, with diameters ranging from capillaries of 8 μm to large arteries of 1 cm. The main function of blood vessels is to transport various nutrients and metabolites in the body. In vitro vascular models provide new tools for basic research and translational medicine. These models can be used to study the effects of biochemical stimuli and physical changes such as flow rate and pressure on the structure and function of endothelial cells, mechanisms of angiogenesis and angiogenesis, Study drug transport, absorption and therapeutic effects, etc. [0003] At present, the constructi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/06
CPCA61F2/06
Inventor 万玮王立秋李艳陈成敏王雪莹
Owner ENERGY RES INST OF SHANDONG ACAD OF SCI
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