Method and device for synthesizing stalactite and stalagmite through microorganisms

A microbial synthesis and stalactite technology, applied in the direction of microorganism-based methods, enzymology/microbiology devices, biochemical cleaning devices, etc., to achieve the effect of not easy to damage, durable and high efficiency

Pending Publication Date: 2018-07-24
HOHAI UNIV
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Problems solved by technology

[0004] In order to overcome the difficult problem of producing artificial stalactites in the prior art, the present invention provides a method and device for microbially synthesizing stalactites and stalagmites. The artificial stalactites and stalagmites are produced through the combination process of microbial hydrolyzed urea and calcium salts, without environmental pollution. Compared with the direct use of chemical methods in the existing technology, it has stronger controllability

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  • Method and device for synthesizing stalactite and stalagmite through microorganisms
  • Method and device for synthesizing stalactite and stalagmite through microorganisms
  • Method and device for synthesizing stalactite and stalagmite through microorganisms

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Embodiment 1

[0045] like Figure 1-8 As shown, a method for microbial synthesis of stalactites and stalagmites, using aerobic microorganisms to hydrolyze urea and the combination process of calcium salts, including microbial enrichment culture, microbial hydrolysis of urea to induce calcium carbonate to produce precipitation, two parts to synthesize stalactites and stalagmites 2, specifically including The following steps:

[0046] Step 1. Microbial enrichment culture

[0047] Step 1.1, prepare bacterial liquid

[0048] Aerobic microorganisms were inoculated into the medium for proliferation and culture to 10 7 ~10 9 cells / L, centrifuge the microorganisms that have grown to the exponential phase at 2-5°C for 10 minutes, then pour off the supernatant that accounts for 0.1-0.5 times the total volume and add a sodium chloride solution with a concentration of 9g / L to make the microbial concentration reach 10 8 ~10 12 a / L.

[0049] In step 1.1, the microorganisms enriched and cultivated ...

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Abstract

The invention discloses a method and device for synthesizing stalactite and stalagmite through microorganisms. A combination process of aerobic microorganism hydrolyzed urea and calcium salt comprisestwo parts including microorganism enrichment culture and a process of hydrolyzing the urea by the microorganisms to induce calcium carbonate to generate sediment to synthesize the stalactite and thestalagmite with solid structures. According to the method and device for synthesizing the stalactite and the stalagmite through the microorganisms, provided by the invention, the needed stalactite andstalagmite are synthesized by utilizing the combination process of the aerobic microorganism hydrolyzed urea and the calcium salt and pollution to the environment is not caused; the speed of synthesizing the artificial stalactite and stalagmite and generating the calcium carbonate in a microorganism reaction process is manually interfered; compared with a manner of manually synthesizing by directly utilizing a chemical method in the prior art, the method has better controllability and the synthesized artificial stalactite and stalagmite can influence crystal form of calcium carbonate sedimentand sizes of the synthesized artificial stalactite and stalagmite through controlling microorganism reaction activity, the dosage of reactants and the like.

Description

technical field [0001] The invention relates to the field of environmental rock and soil, in particular to a method and a device for synthesizing stalactites and stalagmites by microorganisms. Background technique [0002] Stalactite, also known as stalactite, refers to the general term for stalactites, stalagmites, stone pillars and other different forms of calcium carbonate precipitates formed in caves in carbonate rock areas during the long geological history and under specific geological conditions. Its formation often requires Tens of thousands or hundreds of thousands of years. Due to the long time of formation, stalactites have important research value for ancient geological investigations. In limestone, water containing carbon dioxide seeps into the cracks of limestone and dissolves the calcium carbonate in it. When the water dissolved in calcium carbonate drips from the top of the cave, Due to the evaporation of water, the escape of carbon dioxide, etc., the dissol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12M1/04C12M1/36C12M1/38C12R1/07C12R1/01
CPCC12P3/00C12M29/06C12M41/12C12M41/26C12M41/48
Inventor 何稼杨丰高玉峰高云起
Owner HOHAI UNIV
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