Marker combination, primer group and kit for detecting microsatellite sequence stability

A technology of markers and primer sets, applied in the fields of biology and medicine, can solve the problem of PD-1 treatment not showing curative effect, and achieve accurate and high-sensitivity effects of medication guidance and prognosis prediction

Inactive Publication Date: 2018-07-24
北京信诺佰世医学检验所有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The latest clinical research data show that MSI-H patients with metastatic colorectal cancer (mCRC) show a high response rate to PD-1 immunotherapy, while non-MSI-H mCRC patients show no response to PD-1 therapy

Method used

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  • Marker combination, primer group and kit for detecting microsatellite sequence stability
  • Marker combination, primer group and kit for detecting microsatellite sequence stability
  • Marker combination, primer group and kit for detecting microsatellite sequence stability

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Effect test

Embodiment 1

[0107] According to the above-mentioned gDNA extraction method, the DNA of patients A, B and C (referred to as A sample, B sample and C sample) were extracted respectively, wherein B and C have been diagnosed as tumor patients. Capillary electrophoresis detection was performed after amplification according to the PCR process.

[0108] From electrophoresis results Figure 1-3 It can be seen that the detection result of sample A is MSS. From electrophoresis results Figure 4-9 It can be seen that sample B is MSI-H and sample C is MSI-H. The test results are consistent with the real situation of the three patients, and the medication adjustments are made according to the test results of B and C.

Embodiment 2

[0110] According to the above-mentioned gDNA extraction method, the DNA of tumor cells and normal cells of patient D were respectively extracted, amplified according to the PCR process, and then detected by capillary electrophoresis.

[0111] electrophoresis Figure 10-12 For the MSI detection results of the patient's tumor cells, electrophoresis Figure 13-15 It is the MSI detection result of the patient's normal cells, so it can be concluded that the patient is MSI-H. The test result is consistent with the real situation of the patient, and the medication adjustment is also made according to the MSI test result.

[0112] Compared with the 1997 NCI panel, this method uses the detection of monomorphic nucleotide repeat sites, which is in line with the 2002 version of NCI recommended detection standards. At the same time, during electrophoresis, the monomorphic nucleotide repeat sites are grouped for electrophoresis, which makes it easier to interpret the peak diagram.

[01...

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Abstract

The invention belongs to the fields of biology and medical treatment, and provides a marker combination, a primer group and a kit for detecting microsatellite sequence stability. The marker combination consists of markers BAT25, BAT26, NR21, NR24, NR27 and MONO27. The primer group is used for amplifying the markers. The marker combination disclosed by the invention has higher sensitivity, an effect is direct and clear, and more accurate analysis and suggestion can be provided for medication guiding and prognostic prediction of a patient.

Description

technical field [0001] The invention belongs to the fields of biology and medicine, and relates to auxiliary detection of colorectal cancer. Background technique [0002] Colorectal cancer (CRC) is one of the tumors with high mortality and morbidity worldwide, and about 25% of CRCs are genetically related. Hereditary nonpolyposis colorectal cancer (HNPCC), the most dominant type of hereditary colorectal cancer, is a dominant genetic disease caused by DNA mismatch repair gene (MMR) mutations, accounting for about 3% of CRC ~5%. [0003] HNPCC has an early onset (~45 years old) and rapid disease progression, but its prognosis is better than other types of colorectal cancer. The most important feature of HNPCC is that patients carry MMR gene mutations. People with MMR gene mutations have a lifetime risk of CRC as high as 80%. They are also high-risk groups for endometrial cancer, ovarian cancer, and gastric cancer. Therefore, early diagnosis of HNPCC is very important for pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6886C12Q2600/156C12Q2525/151
Inventor 王明旭王冬石旺
Owner 北京信诺佰世医学检验所有限公司
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