Plant-disease-free insect-pest-free synchronous germination treatment method for Codonopsis pilosula seeds
A treatment method and technology of pests and diseases, applied in the direction of seed and rhizome treatment, seed immunity, application, etc., to achieve the effect of improving germination rate, simple operation, and improving seedling uniformity
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Embodiment 1
[0013] 1) Codonopsis pilosula seeds were irradiated with gamma rays, the radiation dose was 0.8 kGy, and the irradiation time was 15 minutes;
[0014] 2) Soak the irradiated Codonopsis pilosula seeds in 30°C water for 5 minutes;
[0015] 3) Soak the above-treated seeds in a calcium chloride solution with a concentration of 3 mM for 12 hours;
[0016] 4) Put the Codonopsis pilosula seeds treated above on the water-soaked gauze, put them in the incubator, and germinate under the light at 28°C, the light intensity is 2000 lux, and the photoperiod is 8 hours. During light germination, the gauze was supplemented with water to keep it moist. Germination began on the 3rd day after treatment, the germination rate reached 93.9% on the 7th day, and the disease incidence rate was 0.23%.
Embodiment 2
[0018] 1) Use gamma rays to irradiate Codonopsis pilosula seeds, the radiation dose is 1.5kGy, and the irradiation time is 10 minutes;
[0019] 2) Soak the irradiated Codonopsis pilosula seeds in 40°C water for 10 minutes;
[0020] 3) Soak the Codonopsis pilosula seeds treated above with 5 mM calcium chloride solution for 24 hours;
[0021] 4) Put the Codonopsis pilosula seeds treated above on the water-soaked gauze, place them in an incubator, and germinate under light at 28°C with a light intensity of 3000 lux and a photoperiod of 12 hours. During light germination, the gauze was supplemented with water to keep it moist. Germination began on the 3rd day of treatment, and the germination rate reached 95.6% on the 7th day. The incidence rate is 0.20%.
Embodiment 3
[0023] 1) Codonopsis pilosula seeds were irradiated with gamma rays, the radiation dose was 2.0kGy, and the irradiation time was 8 minutes;
[0024] 2) Soak the irradiated Codonopsis pilosula seeds in 45°C water for 15 minutes;
[0025] 3) Soak the above-treated Codonopsis pilosula seeds in a calcium chloride solution with a concentration of 10 mM for 36 hours;
[0026] 4) Put the Codonopsis pilosula seeds treated above on the water-soaked gauze, put them in the incubator, and germinate under the light at 28°C, the light intensity is 4000 lux, and the photoperiod is 10 hours. It began to germinate on the third day after treatment, and the germination rate reached 97.3% on the seventh day, and the disease incidence rate was 0.09%.
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