Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for constructing engineering strain for producing flavin mononucleotide, and applications of engineering strain

A single nucleotide and engineering strain technology, applied in the field of bioengineering, can solve problems such as difficult to realize microbial fermentation production of flavin mononucleotide, achieve the effect of realizing fermentation output value, eliminating by-products, and reducing production costs

Inactive Publication Date: 2018-07-27
BEIHANG UNIV
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the conventional genetic engineering idea of ​​"excessive accumulation and secretion in cells" is difficult to realize the microbial fermentation production of flavin mononucleotide

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for constructing engineering strain for producing flavin mononucleotide, and applications of engineering strain
  • Method for constructing engineering strain for producing flavin mononucleotide, and applications of engineering strain
  • Method for constructing engineering strain for producing flavin mononucleotide, and applications of engineering strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Construction of plasmids for riboflavin synthesis pathway:

[0035] The codon-optimized riboflavin synthesis proteins RibA, RibD, RibE and RibH of Bacillus subtilis were introduced into Escherichia coli to construct recombinant Escherichia coli that over-produced riboflavin.

[0036] like figure 2 As shown in middle a, each exogenous gene is designed and synthesized into the form of biological bricks. EcoRI, XbaI restriction site and ribosome binding site (ribosomebinding site, RBS for short) are sequentially designed upstream of the coding sequence of each gene. Wherein, the RBS sequence is: AAAGAGGA GAAA. The ribosome binding site is 6 bp away from the ATG site of the coding sequence. SpeI and PstI restriction sites are designed downstream of the coding sequence of each gene. Order and synthesize the genes ribA, ribD, ribE, and ribH (sequences shown in SEQ ID No.1-4) in the form of bio-bricks from a gene synthesis service company, and separately link them into...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for constructing an engineering strain for producing flavin mononucleotide, and applications of the engineering strain. According to the method, by using a synthetic biology technology, an intracellular flavinpool component and endometrial secretion are regulated in newly-constructed or existing riboflavin fermenting bacteria, and an inside-cell-membrane-interstitium enzyme catalytic step is novelly added, such that the high-purity flavin mononucleotide is produced through fermentation with microorganisms; and the method specifically comprises: (1) introducinga bifunctional enzyme riboflavin kinase / flavin adenine dinucleotide producing enzyme into riboflavin fermentation bacteria to convert the intracellular flavinpool component from riboflavin to flavin adenine dinucleotide, (2) introducing a flavin adenine dinucleotide internal membrane transporter, and transporting the intracellular flavin adenine dinucleotide into cell membrane interstitium, and (3) introducing 5'-nucleotidase to lyse and convert the flavin adenine dinucleotide in the cell membrane interstitium into the flavin mononucleotide. According to the present invention, under the shaking fermentation condition, the fermentation purity of the flavin mononucleotide (accounting for the total flavin compounds) can be up to 92.4%.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for constructing an engineering bacterium producing flavin mononucleotide and its application. Background technique [0002] Flavin mononucleotide (FMN for short) is an expensive compound with a wide range of uses. In organisms, flavoprotein mononucleotide, as a coenzyme of flavoprotein, participates in the redox reaction of cells and plays an important role in basic metabolism. Many microorganisms and plants can synthesize flavin compounds by themselves, while higher animals can only ingest them from food. When they are deficient, they can lead to hair loss, skin inflammation, vision impairment and other diseases. Therefore, as a water-soluble vitamin, flavin mononucleotide is often used as clinical medicine, food and feed additive, food coloring, etc. [0003] In addition, flavin mononucleotide has a redox active center and is an important electron transfer ca...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/63C12P19/32
CPCC12N15/63C12N2800/101C12N2800/22C12P19/32
Inventor 王华杨昀郭林
Owner BEIHANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com