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A kind of preparation method of the oligosaccharide that promotes the proliferation of intestinal probiotics

A technology of intestinal probiotics and oligosaccharides, which is applied in the preparation of oligosaccharides and sugar derivatives, chemical instruments and methods, etc.

Active Publication Date: 2021-09-14
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the preparation and functional activity of isogalacto-oligosaccharides (HeOS) at home and abroad.

Method used

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  • A kind of preparation method of the oligosaccharide that promotes the proliferation of intestinal probiotics
  • A kind of preparation method of the oligosaccharide that promotes the proliferation of intestinal probiotics
  • A kind of preparation method of the oligosaccharide that promotes the proliferation of intestinal probiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Put lactose and melibiose receptors in a screw bottle at a ratio of 1:2, add 50mmol / L phosphate buffer solution with pH 6.5 to adjust the total sugar concentration to 40%, and process under high pressure (121°C, 103Kpa, 20min) , let it completely dissolve, cool naturally to the reaction temperature of 40°C, and keep warm in a water bath for 30 minutes; weigh an appropriate amount of β-galactosidase and add it to the above reaction system to make it reach a final concentration of (10U / mL) and place it on a constant temperature water bath shaker ( 150r / min) for the reaction; after 14 hours of reaction, the boiling water bath was used to inactivate the enzyme for 5 minutes to terminate the reaction; the above reaction product was classified and purified by activated carbon-diatomaceous earth adsorption chromatography to remove monosaccharides and disaccharides, and the purified product was diluted After freeze-drying, a novel functional oligosaccharide is obtained.

[0044...

Embodiment 2

[0048] Put lactose and cellobiose receptors in a screw bottle at a ratio of 1:3, add a 50mmol / L phosphate buffer solution with pH 7.0 to adjust the total sugar concentration to 50%, and process under high pressure (121°C, 103Kpa, 20min) , to make it completely dissolved, then cool naturally to the reaction temperature of 44°C, and keep it warm in a water bath for 30 minutes; weigh an appropriate amount of β-galactosidase and add it to the above reaction system to make it reach a final concentration of (15U / mL) and place it in a constant temperature water bath shaker ( 200r / min) for the reaction; after 12 hours of reaction, the boiling water bath was used to inactivate the enzyme for 5 minutes to terminate the reaction; the above reaction product was classified and purified by activated carbon-diatomaceous earth adsorption chromatography to remove monosaccharides and disaccharides, and the purified product was diluted After freeze-drying, a novel functional oligosaccharide is ob...

Embodiment 3

[0053] Put the lactose and trehalose acceptors in a screw bottle at a ratio of 1:4, add 50mmol / L phosphate buffer solution with pH 7.5 to adjust the total sugar concentration to 60%, after high pressure treatment (121°C, 103Kpa, 20min), Let it completely dissolve and cool naturally to the reaction temperature of 42°C, and keep it warm in a water bath for 30 minutes; weigh an appropriate amount of β-galactosidase and add it to the above reaction system to make it reach a final concentration of (20U / mL) and place it in a constant temperature water bath shaker (250r After 10 hours of reaction, the boiling water bath was used to kill the enzyme for 5 minutes to terminate the reaction; the above reaction products were classified and purified by activated carbon-diatomaceous earth adsorption chromatography to remove monosaccharides and disaccharides, and the purified products were diluted Freeze-dry to obtain novel functional oligosaccharides.

[0054] The yield of oligosaccharides ...

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Abstract

The invention discloses a method for preparing oligosaccharides that promote the proliferation of intestinal probiotics. According to the selection specificity of enzymes for different substrates, common low-lying sugars such as lactose, cellobiose, melibiose, and trehalose are used. Polydisaccharides were used as mixed substrates, and the β-galactosidase from Lactobacillus plantarum 70810 was used to catalyze the transglycosylation reaction. The obtained products were classified and purified by activated carbon β-diatomaceous earth adsorption chromatography to obtain various hetero-oligomeric Galactose mixture. The preparation method is simple in process and low in cost, and the content of oligosaccharides with more than three molecules in the oligosaccharides prepared by the method is more than 95%, and shows significant proliferation function activity on intestinal probiotics, and inhibits harmful bacteria at the same time Growth, almost no monosaccharides and disaccharides in the product, expanding the user population and application fields, high safety, and can be directly applied to various food additives.

Description

technical field [0001] The present invention relates to the field of production of functional oligosaccharides, more specifically, to a method for preparing oligosaccharides that promote the proliferation of intestinal probiotics. Background technique [0002] A variety of bacteria inhabiting the intestines of humans and animals constitute the intestinal bacterial system. Bifidobacteria and lactic acid bacteria are the few beneficial microorganisms in the human intestinal flora that do not produce endotoxins and exotoxins and are non-pathogenic. A large number of experimental results show that the proliferation of bifidobacteria and lactic acid bacteria in the intestinal tract can increase the body's antibody level, activate the phagocytic activity of macrophages, and improve the ability to resist infection. They are the most beneficial bacteria in the intestinal tract that can promote human health. Since the human gastrointestinal tract lacks an enzyme system to hydrolyze f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/18C07H3/06C07H1/06
CPCC07H1/06C07H3/06C12P19/18
Inventor 张宏志周剑忠王英刘小莉夏秀东李亚辉魏照辉
Owner JIANGSU ACAD OF AGRI SCI
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