LAMP primer set and detection method for duck-derived components

Abstract

The invention relates to a LAMP primer set and a detection method for duck-derived components, wherein the LAMP primer set comprises external primers F3 and B3, and internal primers FIP and BIP. According to the present invention, the gene fragment targeted by the LAMP primer sequence set has no crossover with other species such as cattle, pigs, chickens and sheep, and has high specificity in thedetection of duck-derived components; and the LAMP rapid detection method for the duck-derived components has characteristics of convenience, rapidness, short reaction time, low requirement on instrument and equipment, high specificity and high sensitivity, can judge the results by naked eyes, can achieve the visualization of on-site test results, and is suitable for on-site rapid diagnosis.

Description

technical field [0001] The invention relates to the detection field of meat products, in particular to a LAMP primer set and a detection method of duck-derived components. Background technique [0002] Food safety is related to the health and life safety of ordinary people, and to the stability of society. Meat products are the main source of animal protein in daily life, including: fresh meat (cut meat, meat rolls), cured meat (bacon, sausage, ham, etc.), in addition to sauced meat, bacon, jerky, meat Preserved meat, meatballs, kebabs, etc. [0003] Since meat products cannot have the specificity of the overall shape of the animal, once processed, it is even more difficult for consumers to distinguish between genuine and fake. In the face of the current confusion in the beef and mutton market, the traditional method of identifying meat based on its color, smell, texture, fat and thinness can no longer meet the needs of functional department supervision. [0004] In order...

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Problems solved by technology

Zhang Chi et al. designed duck-specific primers and TaqMan probes based on the cytochrome b gene, and the detection sensitivity reached 1.78pg (Fluorescence quantitative PCR detection of duck-derived components in meat products, Food Science, 2013, 34); Li Yingying et al. Methods The relatively species-specific peptides in 5 common edible meats were studied, and the peptides characteristic of the mixed species of mutton and duck were verified and quantified by liquid chromatography and mass spectrometry, and the detection limit could reach 0.5%. (Identification of duck adulteration in mutton by liquid chromatography-tandem mass spectrometry, Food Science, 2016, 37), however, these detection methods must also rely on laboratory instruments, and it is difficult to achieve on-site detection without the laboratory

[0007] Whether it is PCR method, spectrometry or mass spectrometry, it is inseparable from experimental instruments, and the detection needs to be completed in the laboratory. There are many experimental steps, long time and high cost. In the process of meat product quality supervision, it is very necessary to establish A Rapid Detection Technology Realizes Field Detection

[0008] Isothermal Amplification Technology (Isothermal Amplification Technology) is a derivative technology of PCR technology. The reaction process is always maintained at a constant temperature. The purpose of rapid nucleic acid amplification is achieved by adding enzymes with different activities and their respective specific primers. Loop-mediated isothermal amplification (LAMP) is one of them, and it is used in the detection of diseases caused by various viruses, bacteria, parasites, food and cosmetic safety inspections, and rapid diagnosis of import and export. However, the existing The detection method of duck-derived LAMP has many experimental steps, takes a long time and costs a lot

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