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Nucleic acid transfection method based on mofs pore confinement effect

A nucleic acid and effect technology, applied in the field of biomolecular carriers, can solve the problems of low transfection efficiency of immune cells, inability to realize regulation and improvement, difficult and efficient transfection of immune cells, etc., and achieve the effect of good universality and operability.

Active Publication Date: 2022-08-05
WUHAN UNIV
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Problems solved by technology

In terms of transfection, immune cells have always been relatively difficult to achieve high-efficiency transfection. Generally, non-viral vectors have low transfection efficiency for immune cells, and precise regulation and improvement cannot be achieved.

Method used

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  • Nucleic acid transfection method based on mofs pore confinement effect
  • Nucleic acid transfection method based on mofs pore confinement effect
  • Nucleic acid transfection method based on mofs pore confinement effect

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Embodiment Construction

[0033] In order to make the present invention easier to understand, specific embodiments of the present invention will be further described below.

[0034] Preparation of MOFs:

[0035] The 2,5-dihydroxyterephthalic acid was extended by Suzuki coupling reaction to synthesize ligands with 2, 3, 4, and 5 benzene rings, respectively. The ligands and nickel nitrate hexahydrate were added to N,N- In dimethylformamide, ethanol, water and triethylamine were added, and after stirring, solvent exchange, drying, and activation, Ni-IRMOF-74 series samples (referred to as Ni-IRMOF-74-II, Ni-IRMOF-74-II, Ni-IRMOF -74-III, Ni-IRMOF-74-IV, Ni-IRMOF-74-V).

[0036] Loading of nucleic acid molecules:

[0037] The obtained MOFs material is added into the fluorescently labeled nucleic acid solution, and after shaking, centrifugation and washing, the MOFs material loaded with nucleic acid molecules is obtained.

[0038] The nucleic acid molecules can be further expanded into biological macromo...

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Abstract

The present invention provides a nucleic acid transfection method based on the confinement effect of MOFs pores. By selecting a one-dimensional pore MOFs system as a transfection carrier for immune cells that are difficult to achieve efficient transfection, nucleic acid molecules can be successfully loaded through the confinement effect of the MOFs material. , and successfully carried out transfection experiments on a variety of immune cells. Compared with traditional transfection reagents, it has higher efficiency, lower cost and less toxicity, and has a good protective effect on the nucleic acid molecules loaded therein. It will not be decomposed in serum, which provides a basis for the design of universally transfected MOFs materials.

Description

technical field [0001] The invention belongs to the field of biomolecule carriers, in particular to a method for efficiently transfecting immune cells that are difficult to transfect with conventional transfection reagents after using MOFs to load nucleic acid molecules. Background technique [0002] Metal-organic frameworks [Metal-organic Frameworks, MOFs, also known as metal-organic frameworks, metal-organic frameworks, metal-organic complexes or coordination polymers] are composed of inorganic metal ions or metal clusters and organic ligands. The connected crystalline porous material has both the common characteristics of organic materials and inorganic materials. A series of IRMOF-74 with mesoporous structure have been reported by Yaghi in 2012. (H. Deng, S. Grunder, K.E. Cordova, C. Valente, H. Furukawa, M. Hmadeh, F. Gandara, A. C. Whalley, Z. Liu, S. Asahina, H. Kazumori, M. O'Keffe, O . Terasaki, J. F. Stoddart, O. M. Yaghi, Science. 2012, 336, 1018) its pore siz...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/87
CPCC12N15/87
Inventor 邓鹤翔周翔彭双别秉霖
Owner WUHAN UNIV
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