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Extraction method of Luo Han Guo prebiotics and method for simultaneously extracting mogroside V

A technology of mogroside and fruit prebiotics, which is applied in the direction of chemical instruments and methods, glycoside steroids, oligosaccharides, etc., can solve the problem of insufficient utilization of mogrosan fruit resources, and achieve low cost, strong operability, and zero pollution Effect

Active Publication Date: 2020-10-16
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] At present, for the application of Luo Han Guo, most of them still stay in the medicinal or extraction of natural sweetener-Mogroside. The rich prebiotics in Luo Han Guo have been ignored, and the limited resources of Luo Han Guo have not been fully utilized. It is urgent to find a kind of Luo Han Guo Method for extracting prebiotics and method for simultaneously extracting mogroside V

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] (1) Ice-water extraction: crush 100kg of Luo Han Guo fresh fruit, stir and extract with 400kg of ice water at 5°C for 60 minutes, and coarsely filter to obtain the ice-water extract;

[0058] (2) Alkalinization: use a saturated aqueous solution of calcium hydroxide to adjust the pH value of the ice-water extract obtained in step (1) to 9, and filter to obtain an alkalized filtrate;

[0059] (3) macroporous adsorption resin chromatography: put the alkalized filtrate obtained in step (2) at a flow rate of 0.5BV / h on the LX-28 type macroporous adsorption resin chromatography column (the consumption of LX-28 type resin is 5kg, The height-to-diameter ratio of the chromatographic column is 2:1), and the effluent is collected;

[0060] (4) Nanofiltration, decolorization, concentration, and drying: filter the effluent obtained in step (3) with a nanofiltration membrane with a molecular weight cut-off of 200Da, and filter at a pressure of 3.0 MPa. h, put on the D945 type decolo...

Embodiment 2

[0066] (1) Ice-water extraction: crush 150kg of Luo Han Guo fresh fruit, stir and extract with 750kg of ice water at 10°C for 40 minutes, and coarsely filter to obtain the ice-water extract;

[0067] (2) Alkalinization: adjust the pH value of the ice-water extract obtained in step (1) to 10 with a saturated aqueous solution of sodium hydroxide, and filter to obtain an alkalized filtrate;

[0068] (3) Macroporous adsorption resin chromatography: put the alkalized filtrate obtained in step (2) at a flow rate of 1BV / h on a DM-130 type macroporous adsorption resin chromatography column (the amount of DM-130 type resin is 12kg, layer The height-to-diameter ratio of the analysis column is 4:1), and the effluent is collected;

[0069] (4) Nanofiltration, decolorization, concentration, and drying: filter the effluent obtained in step (3) with a nanofiltration membrane with a molecular weight cut-off of 300Da, and filter at a pressure of 2.5MPa. / h, put on the D941 type decolorization...

Embodiment 3

[0075] (1) Ice-water extraction: crush 50kg of Luo Han Guo fresh fruit, stir and extract with 150kg of ice water at 6°C for 60 minutes, and coarsely filter to obtain the ice-water extract;

[0076] (2) Alkalinization: adjust the pH value of the ice-water extract obtained in step (1) to 8 with a saturated aqueous solution of potassium hydroxide, and filter to obtain an alkalized filtrate;

[0077] (3) macroporous adsorption resin chromatography: the alkalized filtrate obtained in step (2) is put on the AB-8 type macroporous adsorption resin chromatography column with a flow rate of 1.5BV / h (the consumption of AB-8 type resin is 2kg, The height-to-diameter ratio of the chromatographic column is 4:1), and the effluent is collected;

[0078](4) Nanofiltration, decolorization, concentration, and drying: filter the effluent obtained in step (3) with a nanofiltration membrane with a molecular weight cut-off of 250Da, and filter at a pressure of 3.0MPa. Collect the retained liquid of ...

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Abstract

The invention relates to a method for extracting momordica grosvenori prebiotics and simultaneously extracting mogrosides V, wherein the momordica grosvenori prebiotics extraction method comprises:(1)grinding momordica grosvenori, extracting with ice water, and filtering; (2) adjusting the pH value to be alkaline and filtering; (3) loading on a large-hole adsorbing resin column, and collecting the effluent liquid ; and (4) filtering by the nanofiltration membrane, and collecting the nanofiltration membrane interception liquid, placing on a decolorizing resin column, collecting the effluent, concentrating and drying to obtain the momordica grosvenori prebiotics. The mogrosides V extraction method comprises the following steps of: washing large-hole adsorbing resin column after loading withwater to neutral, eluting and concentrating; placing on tth reverse phase silica gel column, eluting, collecting target eluate, concentrating, and drying to obtain mogrosides V. The method has the advantages that the content of the obtained momordica grosvenori prebiotics is high to 97.3%, and the yield is high to 89.67%; the content of the obtained mogrosides V is up to 99.5%, and the yield is high to 97.20%; the method has the advantages of simple method, low cost and zero pollution, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting prebiotics and a method for simultaneously extracting sweeteners, in particular to a method for extracting prebiotics from Luo Han Guo and a method for simultaneously extracting mogroside V. Background technique [0002] Luo Han Guo is produced in Guangxi, my country. It is a traditional medicinal health food for clearing heat and detoxifying, reducing phlegm and relieving cough, clearing lung and moistening intestines, promoting body fluid and quenching thirst. It is one of the first batch of medicinal and edible materials approved by the country. Luo Han Guo has outstanding health care effects, and can actively improve and regulate the immune system, liver, and blood sugar of modern people, meeting the health requirements of consumers. [0003] Prebiotics are non-digestible food ingredients that have a beneficial effect on the host by selectively stimulating the growth and activity of bacteria in one o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H3/06C07H3/04C07H3/02C07J17/00C08B37/00
CPCC07H3/02C07H3/04C07H3/06C07J17/005C08B37/0003
Inventor 李伟龙伟岸黄华学
Owner HUNAN HUACHENG BIOTECH
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