Efficient chicken manure fermentation composite bacterium preparation

A technology of combining bacteria and preparations, applied in the field of waste recycling, to achieve the effect of ensuring safe use, low cost and good feeding performance

Active Publication Date: 2018-09-14
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the feed produced by fermentation of chicken manure only pays attention to the changes of crude protein and flavor before and after treatment, and rarely pays attention to the influence of chicken manure fermentation on the content of true protein

Method used

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  • Efficient chicken manure fermentation composite bacterium preparation
  • Efficient chicken manure fermentation composite bacterium preparation
  • Efficient chicken manure fermentation composite bacterium preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: the screening of yeast

[0051] The crude protein content in chicken manure is 25% to 30%, and the crude protein contains more non-protein nitrogen, and the non-protein nitrogen is mainly ammonia and uric acid. Therefore, whether the carbon nitrogen ammonium compounds in chicken manure can be efficiently used for rapid growth and reproduction, and whether non-protein nitrogen can be efficiently converted into true protein is the key to the selection of yeast. Therefore, this embodiment uses the mixed nitrogen, ammonia and uric acid in chicken manure as the only nitrogen source to screen yeast.

[0052] 1. Preparation of medium with different nitrogen sources:

[0053] (1) Minor mineral and trace element solution (mg / L): CaCl 2 2H 2 0 17930;Na 2 HP 4 ·H 2 O 6408; KH 2 PO 4 4272;MgSO 4 ·7H 2 O 4920; FeCl 3 ·6H 2 O 290 ; CuSO 4 ·5H 2 O 20 ; MnSO 4 ·7H 2 O 110.

[0054] (2) chicken manure extract medium:

[0055] Chicken manure leaching liqu...

Embodiment 2

[0091] Embodiment 2: the screening of lactic acid bacteria

[0092] (1) Using the method of fermenting chicken manure in treatment group 5 of Example 1, the chicken manure was aerobically fermented at 30° C., and the fermentation time was 36 hours. Among them, the heat treatment conditions of chicken manure are: 60min at 85°C; 70kg of chicken manure, 15kg of bran, and 15kg of corn flour; after fermentation, it is divided into 5 equal parts.

[0093] (2) Activate three kinds of lactic acid bacteria: Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus sausis, respectively.

[0094] Under sterile conditions, take 100mg of wet weight and inoculate it into a 500ml Erlenmeyer flask for liquid culture, 200rpm, 48h, and cultivate until: the number of live bacteria counted by each lactic acid bacteria reaches 1.0×10 10 Individuals / mL or more.

[0095] (3) According to different proportions of inoculum, each treatment group is as follows:

[0096] Treatment 1: Lactob...

Embodiment 3

[0104] Example 3: Chicken manure fermentation effect display

[0105] 1. Prepare bacterial agent A according to the treatment group 5 of the above-mentioned embodiment 1, and prepare the bacterial agent B according to the treatment group 4 of the embodiment 2. Wherein, according to the liquid volume ratio, Saccharomyces anomalius: Candida tropicalis: Candida utilis=1:2:2; Lactobacillus acidophilus: Lactobacillus plantarum: Lactobacillus sausis=1:1:1.

[0106] 2. Pretreatment of fermentation bottom material: collect fresh chicken manure discharged from healthy chickens for 1-2 days, pick out large pieces of chicken feathers and other foreign matter, divide chicken manure into 4 groups of equal amount for heat treatment: chicken manure at 100 ℃ high temperature condition Under heat treatment for 2 hours; dry heat treatment of bran and corn flour at 80°C for 6 hours. Among them, three groups were used as treatment groups, and the fourth group was used as anaerobic control group....

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Abstract

The invention belongs to the field of waste resource utilization, and particularly relates to an efficient chicken manure fermentation composite bacterium preparation. According to the invention, thepreparation is characterized in that the preparation is prepared from a yeast composition preparation with the total inoculum concentration being 5 percent and a lactobacillus composite preparation with the total inoculum concentration being 5 percent; the yeast composition preparation is prepared from Wickerhamomyces anomalus, candida tropicalis and candida utili according to the inoculum concentration ratio of 1:(2 to 3):(2 to 3), and is used for the aerobic fermentation of the chicken manure; the lactobacillus composite preparation is prepared from lactobacillus acidophilus, lactobacillus plantarum and lactobacillus farciminis according to the inoculum concentration ratio of 1:1:1, and is used for the anaerobic fermentation of the chicken manure. The chicken manure fermentation composite bacterium preparation provided by the invention comprises an aerobic fermentation preparation and an anaerobic fermentation preparation, so that the conventional whole-process anaerobic fermentationidea is broken; the real protein content in the chicken manure is effectively improved; the feeding value of the fermentation chicken manure is increased. The preparation can be directly used in practical production and has the important guidance significance on the subsequent further study.

Description

technical field [0001] The invention belongs to the field of waste recycling, and in particular relates to a combined bacterial preparation for efficiently fermenting chicken manure. Background technique [0002] With the rapid development of the breeding industry in recent years, the scale of laying hens and broiler chickens has continued to expand, and the amount of chicken manure has continued to increase. If it is not treated in time or handled improperly, it will cause pollution to the environment of the farm and its surroundings. The digestive tract of the chicken is short, the feed stays in it for a short time, the feed is not fully digested and utilized, a large amount of nutrients are excreted with the feces, and a considerable part of the nutrients in the feces are still not digested and utilized by the body. Only used as compost, the rich nutrients in it are not fully utilized. [0003] In broiler chicken manure, the crude protein content is 25% to 30%, and the t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/20A23K10/12A23K10/26C12R1/74C12R1/72C12R1/645C12R1/23C12R1/225
CPCA23K10/12A23K10/26C12N1/16C12N1/20
Inventor 李东宋玉慧刘晓风廖银章
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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