Molecular genetic breeding method for California weever
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A genetic breeding and molecular technology, applied in fish farming, climate change adaptation, animal feed, etc., can solve problems such as inbreeding decline, and achieve the effect of large gene pool, clear genetic background, and solving inbreeding decline.
Inactive Publication Date: 2018-09-21
NANJING SHUAIFENG FEED
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[0004] The purpose of the present invention is: in order to overcome the deficiencies in the prior art, obtain a kind of parent with clear and highly pure genetic background, solve the problem of inbreeding depression, the present invention provides a kind of California perch molecular genetic breeding method
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Embodiment 1
[0027] A method for molecular genetic breeding of California perch, said method comprising the following steps:
[0028] Step 1. Basic broodstock selection
[0029] Individuals with strong physique, good color, and good gonad development were selected as broodstock from the original species of California perch and Youzhu No. 1 population for three consecutive generations of population breeding, and the traits were separated after population selection;
[0030] Step 2. Establish a family line
[0031] Select the parent fish with good disease resistance and strong physique after the first step of breeding as the basic breeding group, use molecular marker technology to detect the parental DNA, statistically analyze and calculate the similarity and genetic distance between the parental individuals, and According to the genetic distance between individuals to guide the 1-to-1 pairing breeding of parents, 40 families were constructed by combining genetic distance and morphological ...
Embodiment 2
[0048] A method for molecular genetic breeding of California perch, said method comprising the following steps:
[0049] Step 1. Basic broodstock selection
[0050] Individuals with strong physique, good color, and good gonad development were selected as broodstock from the original species of California perch and Youzhu No. 1 population for three consecutive generations of population breeding, and the traits were separated after population selection;
[0051] Step 2. Establish a family line
[0052] Select the parent fish with good disease resistance and strong physique after the first step of breeding as the basic breeding group, use molecular marker technology to detect the parental DNA, statistically analyze and calculate the similarity and genetic distance between the parental individuals, and According to the genetic distance between individuals to guide the 1-to-1 pairing breeding of parents, 40 families were constructed by combining genetic distance and morphological ...
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Abstract
The invention discloses a molecular genetic breeding method for California weever. The method comprises the steps of selecting basis parent fishes, establishing families, carrying out group breeding selection based on production cells, selecting and breeding parent fishes in the second generation families for hybridization, and repeating the above steps to obtain the third generation fishes. (1) The breeding method can efficiently breed a group with better characters, clear genetic backgrounds, larger gene pool and stable inheritance; (2) the breeding method can quickly screen parents with better characters to solve the problem of inbreeding depression; (3) the breeding method can directly breed fishes and select breeding in a large water body and directly carry out breeding selection under the environmental condition of production breeding, in this way, the result of breeding selection is more representative, the influence of environmental genes on fish growth can be further exerted so that genes of the breeding selection can make complete expression, in addition, the purpose that scientific research is combined with production is achieved, and when the breeding selection is carried out, production can be optimized, thereby acquiring a win-win effect.
Description
technical field [0001] The invention belongs to the technical field of aquaculture and relates to molecular marker-assisted breeding, in particular to a molecular genetic breeding method for California perch. Background technique [0002] California bass, formerly known as largemouth bass, belongs to Perciformes, sunfish family, rich in thiamine, protein, riboflavin, niacin, dietary fiber, vitamin C, vitamin E, vitamin A, cholesterol, carotene, retinoids Alcohol, magnesium, copper, selenium and other nutrients needed by the human body can nourish the liver, kidney, spleen and stomach, and also have curative effects on colds and coughs; in addition, sea bass can also cure fetal movement and less breast milk. Sea bass can not only replenish the body, but will not cause obesity due to overnutrition. It is a good product for body building, blood, spleen and qi, and body health. [0003] Group selection is a commonly used method for breeding in production units. It has the advan...
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