Immobilized composite bacterial agent and application thereof to prevention and treatment on water hyacinth in water

A technology of compound bacterial agent and immobilized carrier, which is applied in the field of microorganisms, can solve serious and environmental pollution problems, and achieve the effects of good effect, environmental protection, and convenient preparation

Inactive Publication Date: 2018-09-28
杨敏杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of chemical herbicides is prone to a new round of environmental pollution, and the consequences are becoming more and more serious

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] An immobilized composite bacterial agent comprises a composite microbial bacterial agent and an immobilized carrier.

[0024] The composite microbial bacterial agent is composed of heterotrophic nitrifying bacteria-Acinetobacter, Candida and Bacillus subtilis.

[0025] The preparation method of the composite microbial agent is as follows: respectively culturing Acinetobacter, Candida, and Bacillus subtilis to a concentration of 1×10 7 bacterium solution / ml, and then mixed according to the volume ratio of 3:1:2, to obtain final product;

[0026] The Acinetobacter SP. is ATCC NO.17978;

[0027] The Candida utilis (Candida utilis) ATCC NO.22023;

[0028] The Bacillus subtilis is (Bacillus subtilis) ATCC NO.6633

[0029] The immobilized carrier is composed of ceramsite and zeolite in a weight ratio of 1:1;

[0030] The immobilized carrier and the composite microbial agent were mixed at a volume ratio of 1:6, and cultured for 48 hours with aeration (stirring speed: 120rm...

Embodiment 2

[0032] Example 2 An immobilized water body purifier, which includes a composite microbial agent and an immobilized carrier.

[0033] The composite microbial bacterial agent is composed of heterotrophic nitrifying bacteria-Acinetobacter, Candida and Bacillus subtilis.

[0034] The preparation method of the composite microbial agent is as follows: respectively culturing Acinetobacter, Candida, and Bacillus subtilis to a concentration of 1×10 7 bacterium solution / ml, then mix according to the volume ratio of 4:2:3, to obtain final product;

[0035] The Acinetobacter SP. is ATCC NO.17978;

[0036] The Candida utilis (Candida utilis) ATCC NO.22023;

[0037] The Bacillus subtilis is (Bacillus subtilis) ATCC NO.6633

[0038] The immobilized carrier is composed of ceramsite and zeolite in a weight ratio of 1:1;

[0039]The immobilized carrier and the composite microbial agent were mixed at a volume ratio of 1:7, and cultured for 48 hours with aeration (stirring speed: 120rmp / Min) ...

Embodiment 3

[0041] Embodiment 3 Composite microbial bacterial agent of the present invention is to water hyacinth pathogenicity test:

[0042] (1) Preparation of compound microbial agent:

[0043] Experimental group: Acinetobacter, Candida, and Bacillus subtilis were cultured according to conventional culture methods to a concentration of 1×10 7 bacterium solution per ml, and then mixed according to the volume ratio of 3:1:2; the Acinetobacter SP. is ATCC NO.17978; the Candida utilis is ATCC NO.22023 ; The Bacillus subtilis is (Bacillus subtilis) ATCC NO.6633;

[0044] Control experiment group 1: Acinetobacter and Candida were cultured according to conventional culture methods to a concentration of 1×10 7 Each / ml of the bacterial liquid, then mixed according to the volume ratio of 3:1; the Acinetobacter is ATCC NO.17978; the Candida is ATCC NO.22023;

[0045] Control experiment group 2: Candida and Bacillus subtilis were cultured according to conventional culture methods to a concentra...

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Abstract

The invention belongs to the technical field of microorganisms, and discloses an immobilized composite bacterial agent. The immobilized composite bacterial agent comprises a composite microorganism bacterial agent and an immobilization carrier; the composite microorganism bacterial agent consists of acinetobacter, candida mycoderma bacteria and bacillus subtilis; the immobilization carrier and thecomposite microorganism bacterial agent are mixed according to a volume ratio of 1 to (6 to 7), and under the condition of 28 to 30 DEG C, aeration culture is carried out to complete microorganism immobilization. The composite bacterial agent disclosed by the invention can obviously reduce a COD (chemical oxygen demand) value of eutrophic water and inhibit mass growth of water hyacinth, has popularization value and is wide in application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to an immobilized composite bacterial agent and its application in preventing and controlling water hyacinth in water bodies. Background technique [0002] Water hyacinth (Eichhornia crassipes Solms) is a perennial aquatic herb of the genus Eichhornia crassipes, also known as water hyacinth, false narcissus, and foreign narcissus. It is native to South America and was introduced to my country in the 1930s. Promoted as livestock and poultry feed. Water hyacinth begins to reproduce at a temperature of 13°C, and can still reproduce at a temperature of 39°C. It has both asexual and sexual reproduction functions, especially asexual reproduction, and its population recovery and diffusion capabilities are extremely strong. Water hyacinth likes to be born in warm, sunny and still water rich in organic matter. Under suitable conditions, a new plant can be reproduced every 5 days...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14C02F3/34C12R1/38C12R1/01C12R1/125
CPCC12N1/20C02F3/34C12N11/14
Inventor 杨敏杰卢珍晶沈华平李震宇
Owner 杨敏杰
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