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Application of a kind of angelica protein in the preparation of auxiliary tumor therapy drugs

A technology of tumor treatment and angelica, applied in the application field of angelica protein in the preparation of adjuvant tumor therapy drugs, to achieve a strong overall protective effect, enhance the activity of GST and GSH-Px, and reduce damage

Active Publication Date: 2021-06-01
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the course of clinical use, amifostine has gradually been found to have greater toxicity, causing most patients to experience adverse reactions such as dizziness, nausea, and vomiting.

Method used

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  • Application of a kind of angelica protein in the preparation of auxiliary tumor therapy drugs
  • Application of a kind of angelica protein in the preparation of auxiliary tumor therapy drugs
  • Application of a kind of angelica protein in the preparation of auxiliary tumor therapy drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 Purification of Angelica ASPR protein

[0036] Soak the Angelica sinensis cut into small pieces in 10 times the volume of 0.05 mol / L Tris-HCl buffer solution (pH 8.0), let it stand overnight at 4 °C for about 15 h, filter the residue with 4 layers of gauze the next day, and filter the filtrate at 12000 rpm, 4 Centrifuge at ℃ for 10 min, and the obtained supernatant is the crude extract of angelica protein; the crude protein solution is subjected to one-step precipitation with ammonium sulfate (0-80%), precipitated overnight and collects the precipitated protein, which is dissolved in 2 times the volume of 0.05 mol / L Tris -HCl buffer solution (pH 8.0), the solution of ammonium sulfate precipitation was loaded on the Sephadex G-50 chromatography column fully equilibrated by the equilibrium solution (0.05 mol / L, Tris-HCl buffer solution of pH 8.0), Elute with equilibrium solution and collect the second elution peak, and check the purity by SDS-PAGE electrophor...

Embodiment 2

[0039] Example 2: Protective effect of Angelica ASPR protein on cisplatin-injured mice

[0040] 1. Experimental animals and grouping

[0041] Male Kunming mice, purchased from Wu's animals, weighing 20 ± 2 g. Normal feeding for three days before the experiment. The mice were randomly divided into 5 groups, 5 mice in each group, each group and its treatment were as follows: the normal group (CON) and the cisplatin-damaged group (DDP) were injected with 0.3 mL of normal saline for 3 days, and 2 hours after the last administration, The CON group was injected with normal saline 0.3 mL, the DDP group was injected with 0.5 mg / mL cisplatin solution 0.3 mL (7.5 mg / kg); )+DDP group and ASPR3 (2.0 mg / mL)+DDP group, mice in the three groups were injected with 0.5 mL of protein solution for 3 days, and then injected with 0.3 mL of cisplatin solution 2 hours after the last administration.

[0042] 2. Detection index---spleen index

[0043] All mice were sacrificed 2 days after modeling...

Embodiment 3

[0048] Example 3: Preventive effect on radioprotection of mice with one-shot whole body irradiation

[0049] 1. Experimental animals

[0050] Male Kunming mice, purchased from Wu's animals, weighing 20 ± 2 g. Drinking water and eating freely, the feed is special irradiated material, and the air is exhausted regularly. There was no abnormality in feeding for three days before the experiment.

[0051] 2. Irradiation conditions and experimental grouping

[0052] The experimental Kunming mice were randomly divided into 5 groups: normal group CON (no administration and no radiation), radiation control group XRT (only radiation without administration), amifostine group (AMFT+XRT) (200 mg / kg , injected 30 minutes before radiation), Angelica ASPR protein group (ASPR+XRT) (1.25 mg / mL), the pre-radiation administration group received continuous intraperitoneal injection of Angelica ASPR protein for 3 days, and the radiation was performed 2 hours after the last injection. The radiati...

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Abstract

The present invention relates to the field of natural traditional Chinese medicine protein, and more specifically relates to the application of an angelica protein in the preparation of an auxiliary tumor treatment drug; the N-terminal sequence of the angelica protein is GIQKTEVEAPSTVSA. The angelica protein is used to prepare auxiliary tumor therapy drugs, and the administration of the angelica protein before chemotherapy can reduce the damage of the chemotherapy drug cisplatin to the spleen of mice; the administration of the angelica protein before radiation therapy can increase the number of peripheral blood leukocytes, thymus Index, spleen index, number of splenic nodules (CFU‑S) and spleen CAT activity were significantly increased by 135%, 103%, 23%, 219% and 180%, respectively, and also increased GST and GSH‑Px in the spleen of irradiated mice Vitality, improve spleen white pulp injury, its overall protective effect is stronger than amifostine, can significantly reduce the weight loss rate of irradiated mice by 94%, thereby improving their quality of life.

Description

technical field [0001] The invention relates to the field of natural traditional Chinese medicine protein, and more specifically relates to the application of an angelica protein in the preparation of adjuvant tumor treatment medicine. Background technique [0002] The chemotherapeutic drug cisplatin (DDP) is one of the platinum-based broad-spectrum anti-cancer specific drugs with clinical activity, but because of its low selectivity, it not only has a strong killing effect on tumor cells, but also inhibits spleen-based Represents the immune system. [0003] Radiation therapy is an effective means of killing tumors, and it undertakes the treatment of 2 / 3 tumor patients. However, the side effects of radiation damage caused by free radicals generated by radiotherapy are large, which seriously weakens the body's local and overall resistance, and patients are often forced to interrupt radiotherapy because of the occurrence of these side effects. Therefore, the prevention and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/10A61P35/00A61P1/14A61K33/243
CPCA61K33/24A61K38/10A61P1/14A61P35/00A61K2300/00
Inventor 潘剑茹王香玲
Owner FUZHOU UNIV
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