Method for high-density culture of D-pantolactone hydrolase producing fungi and application thereof

A pantolactone and high-density culture technology is applied in the field of high-density culture of D-pantolactone hydrolase-producing bacteria, and can solve the problem of low conversion rate of D-pantoate enzyme and conversion of D-pantoate acid. The problems of low fermentation rate, low fermentation density and low enzyme activity can achieve the effect of improving enzyme activity energy, high biomass and enzyme activity, and improving efficiency.

Active Publication Date: 2018-10-09
成都本则生科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for efficient growth of highly concentrated (>10 million cells/ml) pantothenamide producing Bacillus sp.) Lacton B01-19 from anaerobically grown microbial cell lines without losing their ability to produce ambergris during cultivating or growing them under specific conditions such as temperature and oxygen levels. By adjusting certain factors like nutrients concentration, media type, and other variables involved in these processes, this new technique enables more optimal performance compared to previous methods while also increasing the yield of desired products.

Problems solved by technology

This patents describes various ways to improve the production efficiency of α-amino acids such as lanthanide niobiodesulfonic acid(NANS). However, current techniques have limitations including slow decomposition rates due to strong binding between metal cations and other compounds present during processing, difficulty separating complex molecules containing these substances from each others, and limited ability to efficiently convert certain types of amberophiles into their corresponding carboxylic acids through microbe engineering technology.

Method used

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  • Method for high-density culture of D-pantolactone hydrolase producing fungi and application thereof
  • Method for high-density culture of D-pantolactone hydrolase producing fungi and application thereof
  • Method for high-density culture of D-pantolactone hydrolase producing fungi and application thereof

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Embodiment 15

[0026] Embodiment 1 5L fermentation tank fermentation

[0027] The method for high-density cultivation of D-pantolactone hydrolase-producing bacteria described in this embodiment comprises the following steps:

[0028] (1) Inoculate one loop of Fusarium moniliforme through the LB plate culture and activation culture into the seed medium for seed activation, control the culture temperature to 30°C, control the shaker speed to 140rpm, and culture time to 10h , to obtain the seed solution;

[0029] The seed culture medium comprises: glycerin 5g / L, water chestnut pulp 30g / L, peptone 6g / L, asparagine 8g / L, ammonium sulfate 2g / L, adjust pH 7.0-8.0; Cleanly peeled water chestnuts are used as raw materials, which are obtained by adding an equal amount of water through pulping;

[0030] (2) Inoculate the activated seed liquid into a 5L fermenter with 2.5L fermentation medium according to the inoculum amount of 10% and carry out fermentation culture. The control culture temperature is...

Embodiment 25

[0033] Embodiment 2 5L fermentation tank fermentation

[0034] The method for high-density cultivation of D-pantolactone hydrolase-producing bacteria described in this embodiment comprises the following steps:

[0035] (1) Use an inoculation loop to inoculate one loop of Fusarium moniliforme cultured on the LB plate and activated culture into the seed medium for seed activation, control the culture temperature to 25°C, control the shaker speed to 160rpm, and culture time to 10h , to obtain the seed solution;

[0036] The seed culture medium comprises: glycerin 10g / L, water chestnut pulp 20g / L, peptone 10g / L, asparagine 3g / L, ammonium sulfate 4g / L, adjust pH 7.0-8.0; Cleanly peeled water chestnuts are used as raw materials, which are obtained by adding an equal amount of water through pulping;

[0037](2) Inoculate the activated seed liquid into a 5L fermenter with 2.5L fermentation medium according to the inoculation amount of 10%, and carry out fermentation culture. The con...

Embodiment 35

[0040] Example 3 Fermentation in a 5L fermenter

[0041] The method for high-density cultivation of D-pantolactone hydrolase-producing bacteria described in this embodiment comprises the following steps:

[0042] (1) Inoculate one loop of Fusarium moniliforme through the LB plate culture and activation culture into the seed medium for seed activation, control the culture temperature to 28°C, control the shaker speed to 150rpm, and culture time to 10h , to obtain the seed solution;

[0043] The seed culture medium comprises: glycerol 8g / L, water chestnut pulp 25g / L, peptone 8g / L, asparagine 5g / L, ammonium sulfate 3g / L, adjust pH 7.0-8.0; Cleanly peeled water chestnuts are used as raw materials, which are obtained by adding an equal amount of water through pulping;

[0044] (2) Inoculate the activated seed liquid into a 5L fermenter equipped with 2.5L fermentation medium according to 10% inoculum size and carry out fermentation culture. The control culture temperature is 28°C,...

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Abstract

The invention belongs to the technical field of microbial fermentation and enzymatic conversion, particularly relates to a method for high-density culture of D-pantolactone hydrolase producing fungi,and further discloses a method for preparing D-pantoic acid by enzymatic conversion carried out with D-pantolactone hydrolase producing fungi. The method for high-density culture of D-pantolactone hydrolase producing fungi utilizes only conventional fusarium moniliforme in the prior art as an enzyme producing strain, effectively increases the biomass of the enzyme producing strain by optimizing aseed medium and a fermentation medium, and also effectively increases the enzyme of a crude enzyme solution and improves the relative enzyme activity of fungi quantity per unit.

Description

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Claims

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Application Information

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Owner 成都本则生科技有限公司
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