Anti-aging herba dendrobii stem cell extract composition and cosmetics containing composition
A composition and anti-aging technology, applied in the direction of medical preparations, cosmetics, cosmetic preparations containing active ingredients, etc., to enhance the ability to resist oxygen free radicals, improve skin quality, and increase the concentration of SOD
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[0032]The preparation method of the dendrobium stem cell extract of the present invention is as follows: 1) select high-quality dendrobium leaves, clean and disinfect, and cut into powder to make callus; 2) inoculate the callus on a solid medium for induction Cultivate, cut the regenerated part of the callus for subculture, transfer the cultured product to a liquid medium for expanded culture, and then transfer to a bioreactor for large-scale culture until a saturated dendrobium stem cell suspension is obtained; 3) Filter the dendrobium stem cell suspension obtained in 2) with a 1 μm filter, remove the culture medium, and harvest the retained cells; put the cells in ultra-low temperature freezing at -80°C for 2 hours, then remove and thaw for 30 minutes, and repeat the above operation 5 times, after the last thawing at room temperature, break the cells with an ultrasonic breaker, filter through a filter, collect the filtrate, and concentrate it in a rotary vacuum evaporator to ...
Embodiment 1
[0048] An anti-aging composition, comprising stem cell extract of Dendrobium, Atractylodes Rhizoma Atractylodes Extract, honeysuckle juice, turmeric extract; the mass ratio of the stem cell extract of Dendrobium, extract of Rhizoma Atractylodes Rhizome, honeysuckle juice and turmeric extract is 1:15:15:8.
[0049] The composition is prepared as described above.
[0050] The anti-aging composition is used in cosmetics, and the cosmetics include the following ingredients in mass percentage: anti-aging composition 5%, carbomer 0.1%, hydrolyzed sodium hyaluronate 1%, glycerin 10%, trehalose 2%, glycerin hard 3% fatty acid ester, 0.3% potassium sorbate, 1.5% jojoba seed oil, and the balance is deionized water. The preparation method of the cosmetic is as described above.
Embodiment 2
[0052] An anti-aging composition, comprising stem cell extract of Dendrobium, Atractylodes Rhizoma Atractylodes Extract, honeysuckle juice, turmeric extract; the mass ratio of the stem cell extract of Dendrobium, extract of Rhizoma Atractylodes Rhizome, honeysuckle juice and turmeric extract is 1.5:17:10:5.
[0053] The anti-aging composition is used in cosmetics, and the cosmetics include the following ingredients in mass percentage: anti-aging composition 6%, xanthan gum 0.2%, trehalose 15%, cetyl potassium phosphate 1%, phenoxyethanol 0.3% , Shea butter 2%, the balance is deionized water.
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