Piezoelectric ultrasonic microinjection method based on elastic modulus of oocyte

Abstract

The invention discloses a piezoelectric ultrasonic microinjection method based on the elastic modulus of an oocyte. The microinjection method comprises the following steps: S1, measuring the elastic modulus of the oocyte by using a capillary effect balanced pressure model; S2, calculating the piezoelectric microinjection parameter according to the elastic modulus; and S3, controlling the piezoelectric microinjection parameter of a piezoelectric ultrasonic microinjection needle to perform the microinjection on the oocyte under the auxiliary support of a targeted auxiliary sorption needle. By research on the relationship between the elastic modulus parameter of the oocyte and the piezoelectric ultrasonic microinjection parameter of the oocyte, the piezoelectric ultrasonic microinjection parameter is set in real time by using the elastic modulus of the oocyte measured in real time, and the personalized parameter microinjection of the oocyte is really realized.

Description

technical field [0001] The invention relates to the technical field of biological micromanipulation, in particular to a piezoelectric ultrasonic microinjection method based on oocyte elastic modulus. Background technique [0002] Oocyte is the most important cell in the process of life breeding, and the micromanipulation technology of oocyte has always occupied the most important position in life science research and related medical technology. Different oocyte micromanipulation techniques, such as transgene injection technology and somatic cell nuclear transfer technology, have emerged one after another for different developmental stages of oocytes. With the continuous deepening of biomedical technology research, biological micromanipulation technology processes are becoming more and more complex and precise, and the operating objects are becoming more and more high-end, which also puts forward higher and higher requirements for today's micromanipulation technology. [000...

AI Technical Summary

Problems solved by technology

The basic principle is to apply a certain micro force to the cells and obtain the corresponding deformation of the cells; the mechanical parameters of the cells are estimated through the relationship between the force and the deformation, but the cells measured by most of the cell characteristic parameter measurement techniques will lose their activity

However, the current cell micromanipulation technology still simply regards the same kind of cells as "targets" with the same characteristics. Using the same operating parameters to manipulate cells with different characteristics will inevitably result in unsatisfactory results. Due to the particularity of cell micromanipulation, any slight difference in operation may lead to cell death and eventually lead to the failure of the experiment

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