Primary cell culture of gliomas, and construction of patient-derived xenograft (PDX) model

Abstract

The invention discloses primary cell culture of gliomas, and construction of a PDX model. The PDX model has high fidelity and reserves the histopathological and genetic characteristics and tumor heterogeneity of primary gliomas and is a tumor model with the closest relevance to clinical research at present. The PDX model that simulates the specificity of human gliomas has important transformationsignificance to preclinical evaluation, treatment and prognosis of tumors and is expected to bring new breakthroughs in individualized treatment of patients with gliomas.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a glioma primary cell culture and a glioma PDX standardized model library. Background technique [0002] The traditional in vivo drug susceptibility testing method is to screen mouse or human cells in vitro, subculture to establish a stable cell line, and then transplant into mice to establish a tumor model. The application of this model in tumor research has been established. There have been half a century, but this model has certain defects, because the tumor cell lines that have been continuously passaged have adapted to the environment of the external culture dish and lost tumor heterogeneity, so they cannot objectively reflect the situation of the primary tumor. PDX model is a kind of transplantation of patient's fresh tumor cells or tissues directly into immunodeficient mice through orthotopic or heterotopic (mostly subcutaneous or renal capsule), and relying on the environment p...

AI Technical Summary

Problems solved by technology

The PDX models that have been studied more in the early stage are breast cancer, gastric cancer, colon cancer, etc., but there are few studies on the primary culture of tumor tissue and PDX models of glioma

Images