Method and device for freezing optical tweezers cells and particles in liquid nitrogen
A cell and optical tweezers technology, applied in the field of optical tweezers research, can solve the problems of inability to fix cells and tissues, change the physiological properties of cells, damage to cells and tissues, etc., and achieve the effects of reasonable structure, improved accuracy, and avoidance of fixation.
Inactive Publication Date: 2018-10-26
苏州美丽澄电子技术有限公司
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Problems solved by technology
[0002] Optical tweezers is an optical technology that uses light radiation pressure and a single-beam gradient force optical trap to act on the surface of cells and bacteria to generate force. Generally, people use optical tweezers to tweezer cells, bacteria and biological macromolecules. However, current studies have shown that freezing cells with liquid nitrogen can change the physiological characteristics of cells, while ordinary cell clips cannot fix a single cell tissue stably, and are easy to cause damage to cell tissue. The tweezers can fix the experimental cell tissue with optical tweezers, and allow the experimental cells to receive liquid nitrogen freezing in a suspended state in the operation box
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[0039] As a preferred embodiment of the present invention, a support base 8 is provided at the bottom of the condensing and refracting cylinder 2 .
[0040] As a preferred embodiment of the present invention, the electric sealing door 4 is provided with a sealing gas valve, and the inside of the operation box 3 is provided with a refracting curved mirror 15 .
[0041] As a preferred embodiment of the present invention, the number of the light pressure generator 12 and the electric deflection plate 13 are both two.
[0042] As a preferred embodiment of the present invention, the inside of the condensing and refracting tube 2 is provided with a condensing cavity, and the light pressure cavity inside the light radiation press 1 and the condensing cavity inside the condensing and refracting tube 2 are both Seal the vacuum chamber.
[0043] As a preferred embodiment of the present invention, the liquid nitrogen machine 16 is provided with a high-pressure pipe 18 , and the high-pre...
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The invention provides a method and device for freezing optical tweezers cells and particles in liquid nitrogen. The method comprises the following steps of selecting cell tissues, carrying out optical tweezers extraction of cells, suspending the cells, freezing the cells and observing and recording the cells; the device comprises an optical radiation pressure machine, an electronic microscope eyepiece and a liquid nitrogen machine; a bearing sealing plate is arranged at one side of the optical radiation pressure machine; a transmission plate is arranged at the other side of the optical radiation pressure machine; a data line pipe is arranged between the transmission plate and the bearing sealing plate; the method and device for freezing the optical tweezers cells and the particles in theliquid nitrogen are novel in design and reasonable in structure, and can be used for carrying out liquid nitrogen freezing experiments on the cells in a high-accuracy manner.
Description
technical field [0001] The invention relates to a method and a device for freezing optical tweezers cells and particles into liquid nitrogen, and belongs to the technical field of optical tweezers research. Background technique [0002] Optical tweezers is an optical technology that uses light radiation pressure and a single-beam gradient force optical trap to act on the surface of cells and bacteria to generate force. Generally, people use optical tweezers to tweezer cells, bacteria and biological macromolecules. However, current studies have shown that freezing cells with liquid nitrogen can change the physiological characteristics of cells, while ordinary cell clips cannot fix a single cell tissue stably, and are easy to cause damage to cell tissue. The tweezers can fix the experimental cell tissue with optical tweezers, and allow the experimental cells to receive the effect of liquid nitrogen freezing in a suspended state in the operation box. [0003] Therefore, the pr...
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IPC IPC(8): A01N1/02A01N3/00
CPCA01N1/0252A01N3/00
Inventor 林建铃
Owner 苏州美丽澄电子技术有限公司