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Method and device for detecting sodium gluconate content in fermentation broth

A technology of sodium gluconate and a detection method is applied in the field of detection of sodium gluconate content in fermentation broth, can solve problems such as the detection of sodium gluconate yield that cannot be used, achieves good repeatability and stability, widens linear range, and improves operation. Simple process effect

Pending Publication Date: 2018-11-02
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the non-aqueous titration method is a glacial acetic acid reaction system, which can only measure the content of sodium gluconate in solid powder, but cannot directly measure the content of sodium gluconate in aqueous solution, so it cannot be applied to the detection of sodium gluconate production in the fermentation production process

Method used

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  • Method and device for detecting sodium gluconate content in fermentation broth
  • Method and device for detecting sodium gluconate content in fermentation broth
  • Method and device for detecting sodium gluconate content in fermentation broth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Comparative analysis of determination methods of sodium gluconate

[0082] (1) Non-aqueous titration method

[0083] Accurately prepare sodium gluconate-glacial acetic acid standard solutions with concentrations of 0.3g / L, 0.6g / L, 0.9g / L, 1.2g / L, 1.5g / L and 1.8g / L, add 0.05mL mass concentration to 0.1 % quinaldine red-methanol solution was titrated with perchloric acid-glacial acetic acid solution with a concentration of 0.1mol / L until it became colorless, which was the titration end point. Take the sodium gluconate concentration as the abscissa and the titrated volume of the perchloric acid-glacial acetic acid solution as the ordinate to draw a standard curve, see figure 2 .

[0084] The determination principle of the non-aqueous titration method is that sodium gluconate is a weakly alkaline compound, and the glacial acetic acid system can enhance its alkalinity, and an obvious titration jump can be observed when titrated with perchloric acid.

[0085] Such as fi...

Embodiment 2

[0100] (1) Mix sodium gluconate with water, and prepare concentrations of 0mol / L, 0.1mol / L, 0.2mol / L, 0.3mol / L, 0.4mol / L, 0.5mol / L, 0.6mol / L, 0.7 mol / L, 0.8mol / L, 0.9mol / L, 1.0mol / L, 1.1mol / L, 1.2mol / L, 1.3mol / L, 1.4mol / L, 1.5mol / L, 1.6mol / L, 1.7 mol / L, 1.8mol / L, 1.9mol / L, 2.0mol / L, 2.1mol / L, 2.2mol / L, 2.3mol / L and 2.4mol / L sodium gluconate standard solution, take 0.1mL different Add 0.05 mL of quinaldine red-methanol solution with a mass concentration of 0.1% to 10 mL of glacial acetic acid, and titrate with 0.1 mol / L perchloric acid-glacial acetic acid solution until colorless. is the end point of the titration. Take the sodium gluconate concentration as the abscissa and the titrated volume of the perchloric acid-glacial acetic acid solution as the ordinate to draw a standard curve, see Figure 6 .

[0101] Depend on Figure 6 As can be seen, the regression equation y=1.0903x+0.0532 of sodium gluconate content, R 2 =0.9997, the linear relationship is ideal.

[0102] (2...

Embodiment 3

[0136] (1) Mix sodium gluconate with water, the preparation concentration is 0mol / L, 0.2mol / L, 0.4mol / L, 0.6mol / L, 0.8mol / L, 1mol / L, 1.2mol / L, 1.4mol / L, 1.6mol / L, 1.8mol / L, 2mol / L, 2.2mol / L and the sodium gluconate standard solution of 2.4mol / L, the sodium gluconate content in the fermented liquid is carried out by adopting detection device provided by the present invention Determination, take the sodium gluconate standard solution concentration as the abscissa, and take the detection device measurement result as the ordinate to draw a linear relationship curve, see Figure 7 .

[0137] Depend on Figure 7 It can be seen that the detection device measurement result is consistent with the sodium gluconate standard solution concentration, and the regression equation is y=0.9968x+0.0012, R 2 = 0.9999.

[0138] (2) Precision experiment

[0139] The fermentation broth was centrifuged (10000r / min, 10min), and the supernatant was taken as the sample to be tested. The measurement...

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Abstract

The invention provides a method for detecting the sodium gluconate content in fermentation broth, which can accurately determine the sodium gluconate content in the fermentation broth. A methodology experiment shows that the method provided by the invention has a wide linear range, and good precision, repeatability, stability and accuracy. Other components such as glucose in the fermentation brothhave no influence on a determination result, and the method provided by the invention has a simple determination process and is a rapid and accurate method for determining the sodium gluconate content in the fermentation process of aspergillus niger. In addition, the invention provides a device for detecting the sodium gluconate content in the fermentation broth. The detection device provided bythe invention can accurately determine the sodium gluconate content in the fermentation broth, can complete the sample determination within 3 min, has the advantages of simple operation, simple structure and no use of expensive components, and can realize the monitoring of the process of fermenting aspergillus niger and producing sodium gluconate.

Description

technical field [0001] The invention relates to the technical field of compound detection, in particular to a method and device for detecting sodium gluconate content in fermentation broth. Background technique [0002] Sodium Gluconate (C 6 h 11 o 7 Na) is a sodium polyhydroxycarboxylate, its appearance is white or light yellow crystalline powder. Sodium gluconate is easily soluble in water, mild and non-toxic, has a salty taste, and has excellent chelating properties, so it is widely used in food, construction, chemical, pharmaceutical and other fields. In the food industry, sodium gluconate is an important food additive, which can be used as a pH regulator stabilizer, edible salt substitute, and taste improver. In the construction industry, sodium gluconate is used as a water reducer and retarder, which can enhance the strength of concrete and prolong the setting time. In the chemical industry, adding sodium gluconate to the cleaning agent will achieve a very ideal c...

Claims

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Application Information

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IPC IPC(8): G01N31/16G01N21/78G01N1/28G01N1/38
CPCG01N1/28G01N1/38G01N21/78G01N31/16G01N2001/386
Inventor 郑岚马耀宏孟庆军杨艳杨俊慧王丙莲
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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