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Method for detecting anthocyanidin content of blueberries and application

A technology for anthocyanin content and blueberry anthocyanin, which is applied in measuring devices, chemical method analysis, chemical analysis by titration, etc., can solve the problem of different processing methods, no effective analysis method for the determination of blueberry anthocyanin content, Can not solve the problem of determining the total flavonoid content of blueberry anthocyanins and other problems

Inactive Publication Date: 2018-11-02
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] Blueberry anthocyanins are derivatives of flavonoids and flavanones, composed of various flavonoids, and have a purple color. Currently, the pure product content determination methods mainly include ultraviolet absorption at 530-535nm and 192nm The liquid phase analysis method and the determination methods such as n-butanol-hydrochloric acid method, vanillic acid-hydrochloric acid method, etc., but these methods are only for the determination of blueberry anthocyanin content with a single content, and cannot solve the problem of the blueberry anthocyanins extracted due to different growth environments. The problem of determining the content of total flavonoids of blueberry anthocyanins, and the processing methods before the determination are different, and the analysis results are also different. At the same time, the color of blueberry anthocyanins limits the use of these methods
[0004] PVP (polyvinylpyrrolidone) PVP compounds are commonly used gel excipients, the used model is K30 to K90, and the molecular weight distribution is 379-600,000. The PVP compound is easy to swell in water to form a gel property, and the blueberry anthocyanin is made into a transparent gel to increase the blueberry anthocyanin. However, there is no effective analysis method for the determination of blueberry anthocyanin content in the gel

Method used

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  • Method for detecting anthocyanidin content of blueberries and application
  • Method for detecting anthocyanidin content of blueberries and application
  • Method for detecting anthocyanidin content of blueberries and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The establishment of blueberry anthocyanin oxidation curve model, the steps are as follows:

[0047] (1) Precisely weigh 0.1 g of blueberry anthocyanin sample into a 100 mL volumetric flask, add deionized water to dissolve, and prepare three solutions with concentration gradients of 200, 400, 600, 800, and 1000 μg / mL for use.

[0048] (2) Precisely measure 10 mL of blueberry anthocyanin samples with different concentration gradients prepared above and place them in an iodine bottle, accurately add 10 mL of sodium periodate acidification solution and mix, heat at 37°C for 30 minutes, and let cool to room temperature after the reaction is completed;

[0049] (3) Add 5 mL of potassium iodide test solution to (2), seal it tightly, and let it stand for 5 minutes;

[0050] (4) Titrate the solution in (3) with sodium thiosulfate titration solution until the potential changes suddenly, and the titration result is corrected with blank test solution;

[0051] (5) Calculate the c...

Embodiment 2

[0068] Establishment of commercial blueberry anthocyanin oxidation curve model and content detection

[0069] Sample: Commercially available blueberry anthocyanins (batch numbers JH171205, JH180123, JH180307)

[0070] in,

[0071] Blueberry anthocyanin JH171205 was purchased from Xi'an Ruiying Biotechnology Co., Ltd.;

[0072] Blueberry anthocyanin JH180123 was purchased from Shaanxi Senfu Natural Products Co., Ltd.;

[0073] Blueberry anthocyanin JH180307 was purchased from Xi'an Jinheng Chemical Co., Ltd.

[0074] Instrument used: Titralab1000 automatic potentiometric titrator

[0075] Determination of commercial blueberry anthocyanin content:

[0076] Weigh commercially available blueberry anthocyanins (batch numbers JH171205, JH180123, JH180307) in three portions of 1.000 g each, and dilute them into solutions with concentrations of 200, 300, 400, 500, 600, 700, and 800 μg / mL, each with a concentration of 10 mL, Take three sample solutions, put them in iodine measurin...

Embodiment 3

[0081] Establishment of oxidation curve model and content detection of blueberry anthocyanin gel preparation

[0082] (1) Determination of the content of blueberry anthocyanins after mixing with PVP auxiliary materials (taking blueberry anthocyanins with batch number JH171205 as an example):

[0083] Take the prescribed amount of blueberry anthocyanin (batch number JH171205) and mix them with quantitative gel materials (polyvinylpyrrolidone PVPK30, PVPK90), dilute with deionized water, let it stand, take the supernatant, take 10mL, and dilute the blueberry anthocyanin respectively The theoretical concentration is 200, 300, 400, 500, 600, 700, 800 μg / mL for use. Carry out potentiometric titration according to the blueberry anthocyanin potentiometric titration steps in Example 1, and correct the titration result with a blank test solution until near the end point, record the volume of sodium thiosulfate consumed, and calculate the consumption according to the volume of sodium th...

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Abstract

The invention discloses a method for detecting anthocyanidin content of blueberries and application and belongs to the technical field of detection and analysis on effective ingredients. The method for measuring the anthocyanidin content, disclosed by the invention, comprises the steps of adding a sodium periodate acidifying solution into an anthocyanidin sample to be measured by adopting a redoxpotentiometry titration method, performing a reaction on the sodium periodate acidifying solution and the anthocyanidin sample to be measured, measuring the consumption of sodium periodate after the reaction is completed, and introducing the consumption of sodium periodate into an anthocyanidin oxidation curve model, thereby obtaining the anthocyanidin content. The method disclosed by the invention has the characteristics that analysis steps are simple, instruments are convenient to use, and the method is free of influence caused by colors and temperatures and is free of influence caused by gel preparation auxiliary material PVP; the method can be applied to content analysis in industrialized production of blueberry anthocyanidin preparations, has obvious social and economic significance,meanwhile, can be extended to the detecting the content of other polyhydroxyl-contained total anthocyanidins.

Description

technical field [0001] The invention belongs to the technical field of detection and analysis of active ingredients; in particular, it relates to a method and application for detecting blueberry anthocyanin content. Background technique [0002] Anthocyanins are a class of water-soluble pigments that widely exist in plants and belong to flavonoids. In recent years, anthocyanin has attracted attention as a natural pigment extracted by modern biotechnology and has been proven to be the most effective antioxidant discovered by humans. Studies have found that its antioxidant performance is fifty times higher than vitamin E and two times higher than vitamin C. It is 100 times more biologically effective to the human body. It is called "oral skin cosmetics" by Europe and other regions. It can nourish the skin, enhance skin immunity, and deal with various allergic symptoms. [0003] Blueberry anthocyanins are derivatives of flavonoids and flavanones, composed of various flavonoids...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/16
CPCG01N31/164
Inventor 金青王瑞玉范蕊郑洋林欢张丽萍谭心舜
Owner QINGDAO UNIV OF SCI & TECH
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