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Immersion Objectives for Microscopy

A microscope and liquid immersion technology, which is applied in the field of liquid immersion objective lenses for microscopes, can solve the problems of increase, high manufacturing cost, frequent maintenance rate, etc.

Active Publication Date: 2022-01-11
LEICA MICROSYSTEMS CMS GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The comparatively low refractive power of the correction element has the disadvantage that the objective must have a large overall length in order to achieve a large correction effect, and that the diameter of the objective increases with the overall length, which is disadvantageous in many microscopic applications of
This construction is characterized by a high degree of mechanical complexity, with consequent high manufacturing costs and frequent repair rates

Method used

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  • Immersion Objectives for Microscopy
  • Immersion Objectives for Microscopy
  • Immersion Objectives for Microscopy

Examples

Experimental program
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Effect test

Embodiment Construction

[0026] figure 1 The immersion objective 10 is shown in a sectional view along the optical axis O as a possible embodiment.

[0027] The immersion objective 10 faces the sample space 44 in which the sample 42 is arranged. An immersion liquid 40 such as water, glycerin or a special immersion oil is located in the sample space 44 .

[0028] The liquid immersion objective lens 10 comprises the first lens group 12 of positive refractive power, the second lens group 14 of positive refractive power, the third lens group 16 of negative refractive power and the fourth lens group 28 of positive refractive power, and these lens groups are obtained from sample 42 from the sample side , Arrange in this order. Only the second lens group 14 is designed to be movable for correcting spherical aberration, that is to say, the first lens group 12 , the third lens group 16 and the fourth lens group 18 are fixed.

[0029] Viewed from the object sample side, the first lens group 12 includes: a f...

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PUM

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Abstract

A liquid immersion objective lens (10) for a microscope (100) is introduced, comprising a first lens group (12) of positive refractive power, a second lens group (14) of positive refractive power, and a third lens group of negative refractive power (16) and the fourth lens group (18) of positive refractive power, these lens groups are arranged in this order from the object sample side, wherein, in order to realize the correction effect for spherical aberration, the second lens group (14) can be along The optical axis (O) moves, so that the distance (V1) between the second lens group (14) and the first lens group (12) and the distance (V1) between the second lens group (14) and the third The sum of the distances (V2) between the lens groups (16) is constant, wherein the correction effect of the second lens group (14) is predetermined so that the spherical aberration of light projection corresponding to the average numerical aperture At a minimum, the mean numerical aperture lies between zero and the nominal aperture of the immersion objective (10).

Description

technical field [0001] The invention relates to a liquid immersion objective lens for a microscope, which comprises a first lens group of positive refractive power, a second lens group of positive refractive power, a third lens group of negative refractive power and a fourth lens group of positive refractive power, These lens groups are arranged in this order from the object side, wherein the second lens group is movable along the optical axis in order to correct the spherical aberration, so that the distance between the second lens group and the first lens group and The sum of the distances between the second lens group and the third lens group is constant. Background technique [0002] Liquid immersion objectives intended for microscopy are known from the prior art, which allow the operator to correct imaging aberrations caused by the varying optical properties of the sample space. The imaging aberrations are, for example, spherical aberrations, which arise due to density...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/33G02B27/00
CPCG02B21/33G02B27/0068G02B21/02G02B27/0025G02B21/025G02B7/10G02B9/34G02B27/0037
Inventor 克里斯蒂安·舒尔茨克里斯蒂安·舒曼
Owner LEICA MICROSYSTEMS CMS GMBH
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