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Method for isolating quercetin rhamnoside from Artemisia oxtail

A technology of rhamnoside and sagebrush, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc.

Inactive Publication Date: 2021-02-05
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no method for separating quercetin 3-O-α-L-rhamnoside from Artemisia annua using HSCCC

Method used

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  • Method for isolating quercetin rhamnoside from Artemisia oxtail
  • Method for isolating quercetin rhamnoside from Artemisia oxtail
  • Method for isolating quercetin rhamnoside from Artemisia oxtail

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Embodiment 1

[0031] instrument

[0032] TBE-300B HSCCC (Shanghai Tongtian Biotechnology Co., Ltd.); DC-0506 low-temperature constant temperature bath (Shanghai Tongtian Biotechnology Co., Ltd.); prime Plus chromatography (with Prime View workstation, General Electric Medical Systems Co.); Waters Acquity H-Class ultra-high performance liquid chromatography (U.S. Waters company); Waters Quattro Premier XE mass spectrometer (U.S. Waters company); Agilent-400MHz nuclear magnetic resonance instrument (U.S. Agilent Technologies); IR200 Fourier transform infrared spectrometer (U.S. Thermo Necolet company ); TU-1950 series UV-Vis spectrophotometer (Beijing General Analysis General Instrument Co., Ltd.); KQ5200E ultrasonic cleaner (40kHz, 250W, Kunshan Ultrasonic Instrument Co., Ltd.); Germany RV 10 rotary evaporator (German IKA company); FD-1-50 vacuum freeze dryer (Beijing Boyikang Experimental Instrument Co., Ltd.).

[0033] Materials and Reagents

[0034] Artemisia oxtail medicinal materi...

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Abstract

The invention provides a method for separating quercetin 3-O-α-L-rhamnoside from Artemisia sativa, the method comprising: taking n-hexane-ethyl acetate-methanol-water=3:10:3: 10v / v mixed fully, after layering, the upper solvent and the lower solvent were used for later use; the lower solvent was removed to dissolve the extraction part of sagebrush ethyl acetate, and the obtained sample solution was separated by high-speed counter-current chromatography (HSCCC) . The invention adopts HSCCC to extract and isolate quercetin 3-O-α-L-rhamnoside with a purity of more than 90% from Artemisia oxtail more conveniently and quickly, and provides a new way for the preparation of the compound.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of natural plant active ingredients. Background technique [0002] Quercetin 3-O-α-L-rhamnoside is a flavonoid glycoside that mostly exists in natural plants and has a strong antioxidant effect. Its structural formula is as follows: [0003] [0004] High-speed countercurrent chromatography is a new type of liquid-liquid partition chromatography technology developed by Dr. Ito of the National Institutes of Health in the 1980s. Separation is an all-liquid chromatographic separation method that does not require solid adsorbents. [0005] So far, there is no method for separating quercetin 3-O-α-L-rhamnoside from Artemisia annua using HSCCC. Contents of the invention [0006] The object of the present invention is to provide a method for isolating quercetin 3-O-α-L-rhamnoside from Artemisia oxtail. [0007] Specifically, it includes the following operations: [0008] Take ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 刘圆杨正明李莹黄艳菲
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES