Blastocyst culture liquid and preparation method thereof

A technology of culture medium and blastocyst, which is applied in the direction of biochemical equipment and methods, culture process, tissue culture, etc., can solve the problems of inability to effectively ensure the formation of embryo blastocyst and poor blastocyst development, and achieve the goal of reducing toxicity, The effect of maintaining vitality and nutrition and promoting blastocyst formation

Inactive Publication Date: 2018-11-06
瑞柏生物(中国)股份有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At present, the conventional blastocyst culture medium used clinically is the modified human tubal saline solution (HTF) with sodium bicarbonate as

Method used

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Embodiment Construction

[0024] The present invention will be further described below in combination with specific implementation methods.

[0025] Blastocyst culture fluid of the present invention is that alanine, aspartic acid, glutamic acid, glycine, proline, taurine, serine, threonine, tyrosine, methionine, Lipoic acid, arginine, cystine, histidine, isoleucine, leucine, lysine, phenylalanine, threonine, valine, folic acid, inositol, vitamin B12, vitamin B6, vitamin B5, vitamin B2, vitamin B1, thiamine, hypoxanthine, pantothenic acid and chelating agent are dissolved in a saline solution containing antibiotics and indicators.

[0026] In the blastocyst culture medium, alanine, aspartic acid, glutamic acid, glycine, proline, taurine, serine, threonine, tyrosine, methionine, lipoic acid, The concentrations of amino acid, cystine, histidine, isoleucine, leucine, lysine, phenylalanine, threonine, and valine are all 0.02-0.50mmol / L; folic acid, muscle The concentrations of alcohol, vitamin B12, vitami...

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PUM

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Abstract

The invention relates to a blastocyst culture liquid and a preparation method thereof, and belongs to the field of human-assisted reproduction technique. The blastocyst culture liquid is prepared by adding alanine, aspartic acid, glutamic acid, glycine, proline, taurine, serine, threonine, tyrosine, methionine, lipoic acid, arginine, cystine, histidine, isoleucine, leucine, lysine, phenylalanine,threonine, valine, folic acid, inositol, vitamin B12, vitamin B6, vitamin B5, vitamin B2, vitamin B1, vitamin B2, thiamine, hypoxanthine, pantothenic acid and chelating agent into a salt solution. Theblastocyst culture liquid has the advantages that the energy and nutrition of embryo are effectively maintained; several unnecessary heavy metal ions can be chelated by the chelating agent, so that the toxicity of the culture liquid is decreased; the blastocyst culture liquid is suitable for promoting the development of embryo and the formation of blastocyst in the early phase, improving the success rate of assisted reproduction technique, and providing powerful guarantee for the safety of assisted reproduction technique.

Description

technical field [0001] The invention relates to a blastocyst culture solution and a preparation method thereof, belonging to the technical field of human assisted reproduction. Background technique [0002] In human assisted reproductive technology, blastocyst culture medium is the post-cleavage culture medium in the sequential culture medium system, which is mainly used in the human embryo and embryo transfer process from the 3rd day to the 5th / 6th day of culture. At present, the conventional blastocyst culture medium used clinically is the modified human tubal saline solution (HTF) with sodium bicarbonate as the buffer, which cannot effectively ensure the blastocyst formation of embryos, especially for the blastocyst development of early embryos. good. Contents of the invention [0003] The purpose of the present invention is to provide a blastocyst culture solution with various quality control standards, which can not only effectively maintain the vitality of embryos, ...

Claims

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Application Information

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IPC IPC(8): C12N5/073
CPCC12N5/0604C12N2500/30C12N2500/32C12N2500/35C12N2500/38C12N2500/40
Inventor 冯怀亮
Owner 瑞柏生物(中国)股份有限公司
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