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Method for evaluating activity of bacteria after transplanting bacteria into digestive tract

A method for evaluating the activity of bacteria

Active Publication Date: 2018-11-06
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Claims
  • Application Information

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Problems solved by technology

Among them, the culture counting method cannot analyze bacteria that are difficult to culture; nucleic acid-based detection methods cannot distinguish dead bacteria from live bacteria, so the activity of implanted bacteria cannot be accurately evaluated

Method used

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  • Method for evaluating activity of bacteria after transplanting bacteria into digestive tract
  • Method for evaluating activity of bacteria after transplanting bacteria into digestive tract
  • Method for evaluating activity of bacteria after transplanting bacteria into digestive tract

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Embodiment Construction

[0017] With reference to the following detailed description and exemplary embodiments, it should be understood that the application is not limited to the details or methodology set forth in the specification or shown in the drawings.

[0018] refer to figure 1 Shown, in the activity evaluation method of the bacterium that the present invention relates to transplanting into the alimentary canal, in the cultivation process (such as in vivo or in vitro cultivation) before implantation bacterium implantation, add A group marker (or be referred to as the first group marker) so that the cultured and active implanted bacteria are marked with the A group; then the implanted bacteria are transplanted into the digestive tract of the host (such as by gavage), and after a period of colonization (such as 6-12h) , using a marker with a B group (or called a second group marker) to treat the intestinal flora of the recipient after receiving the implanted bacteria, for example, by gavage the r...

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Abstract

The invention relates to a method for evaluating the activity of bacteria after transplanting the bacteria into a digestive tract. The method comprises the following steps: enabling implantation bacteria before transplanting to carry A-group markers; marking and treating an intestinal flora of a receptor receiving the implantation bacteria by utilizing in-vivo metabolism of B-group markers after transplanting the implantation bacteria into the digestive tract for a certain time, thus detecting bacteria which simultaneously carry the A-group markers and the B-group markers and bacteria which just carry one of the A-group markers and the B-group markers of the flora.

Description

technical field [0001] The invention relates to a method for evaluating the activity of bacteria transplanted into the digestive tract, in particular to a method for evaluating the activity of the bacteria transplanted into the digestive tract by using double labeling. Background technique [0002] For the tracking of exogenous bacteria after they are implanted into the host’s intestines, it is possible to carry out non-natural sugar metabolism labeling, couple fluorescent groups and use methods when cultivating specific bacteria in vitro, and then implant them into the host by gavage, etc. In vivo, use its fluorescent group for tracking, such as the literature Nature Medicine.21, 1091-1100 (2015). In addition, it has also been reported that the intestinal flora in the host can be metabolically marked by gavage of the host with unnatural sugar or unnatural D-amino acid (alanine), such as the literature Biochemistry.56,3889 -3893 (2017) and Nat. Microbiol. 2, 17099 (2017). ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/04
Inventor 杨朝勇王炜
Owner RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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