Preparation method of a novel magnetic self-assembled three-dimensional paper chip
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A self-assembly, paper chip technology, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc., to achieve the effect of simplifying preparation steps, reducing production costs, and reducing chip packaging steps
Inactive Publication Date: 2020-11-27
DALIAN UNIV
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This process is often the technical bottleneck to realize the mass production of chips
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Embodiment 1
[0032] This embodiment provides a method for preparing a novel magnetic self-assembled three-dimensional paper chip, comprising the following steps:
[0033] S101, using hydrophobic magnetic ink, and obtaining the channel layer, pretreatment reagent layer 3 and detection layer 5 of the paper chip respectively by screen printing;
[0034] Mix the hydrophobic ink and the magnetic powder material evenly to obtain the hydrophobic magnetic ink, use a scraper to screen-print the hydrophobic magnetic ink on the paper through the patterned printing ink, penetrate into the paper, and form a hydrophobic area, and the unprinted part is a hydrophilic area, respectively Obtain the channel layer, pretreatment reagent layer and detection layer of the paper chip.
[0035] Specifically, a 120-mesh patterned nylon printing ink screen was selected with a screen tension of 15 N / cm, and the designed pattern was printed on filter paper with hydrophobic magnetic ink using the screen, and dried in th...
Embodiment 2
[0045] This embodiment provides a method for preparing a novel magnetic self-assembled three-dimensional paper chip, comprising the following steps:
[0046] S201, using hydrophobic magnetic ink, and obtaining the channel layer of the paper chip, the pretreatment reagent layer 3 and the detection layer 5 respectively by screen printing;
[0047] Select a 100-mesh patterned ink-printed nylon screen, and print the magnetic hydrophobic printing solution on the nitrocellulose membrane. The screen tension during printing is 18N / cm, the composition of the hydrophobic ink is composed of screen printing ink and ethyl acetate with a volume ratio of 3:1, the magnetic powder is 150-250 mesh ferric oxide magnetic powder, and the ratio of hydrophobic ink to magnetic powder is The weight ratio is 1:0.8. The screen distance between the printing ink screen and the nitrocellulose membrane is 1.0mm during printing, the angle of the scraper and the printing ink screen is 50°, the printing speed...
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Abstract
The invention relates to a preparation method of a three-dimensional paper chip, in particular to a preparation method of a novel magnetic self-assembled three-dimensional paper chip. The preparationmethod comprises the following steps: with hydrophobic magnetic ink, carrying out screen printing to obtain a channel layer, a pretreatment reagent layer and a detection layer of the paper chip separately; printing a pretreatment agent on the pretreatment reagent layer with a squeegee through patterned reagent printing silk screen; printing a detection agent in a detection area on the detection layer with a squeegee through the patterned reagent printing silk screen; and stacking the channel layer, the pretreatment reagent layer and the detection layer in sequence on a pad made of a magnetic material, so as to obtain the three-dimensional paper chip. The preparation method of the novel magnetic self-assembled three-dimensional paper chip provided by the invention adopts a screen printing technology, an additional bonding layer or a glue spraying or printing step is avoided by improving a printing material, and a magnetic material and a hydrophobic material are both used for printing tosimplify the preparation steps and eliminate the risk that glue permeates to block hydrophilic channels.
Description
technical field [0001] The invention relates to a preparation method of a three-dimensional paper chip, in particular to a preparation method of a novel magnetic self-assembled three-dimensional paper chip. Background technique [0002] Microfluidic paper chip is a new type of analytical device that processes a fluid channel network on a paper substrate or paper-based material through methods such as water transfer or hydrophilic modification, and can be used for biological and chemical detection and analysis, also known as on-paper Micro-total analysis laboratory. It not only has the advantages of small volume, less reagent consumption, and easy portability of traditional microfluidic chips, but also has the advantages of simple preparation, low cost, and good biocompatibility. It is a new type of micro-analysis technology platform that has emerged in recent years. , has good application prospects in the fields of disease diagnosis, pathogen detection, food safety and envi...
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