A Novel Human Antimicrobial Peptide 11-37 Derivative and Its Application

A human antimicrobial peptide, LL-37 technology, applied in the field of molecular biology, can solve the problems of inability to realize large-scale production, complex process, expensive cost, etc., to solve the problem of antibiotic abuse, good antibacterial effect, and stability improvement Effect

Active Publication Date: 2021-07-30
ZHUHAI INST OF ADVANCED TECH
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the problems of low yield, long time-consuming, complicated process and high cost in extracting natural antimicrobial peptides from animals, it is impossible to achieve large-scale production, which has become the biggest obstacle restricting the clinical application of antimicrobial peptides.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A Novel Human Antimicrobial Peptide 11-37 Derivative and Its Application
  • A Novel Human Antimicrobial Peptide 11-37 Derivative and Its Application
  • A Novel Human Antimicrobial Peptide 11-37 Derivative and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1 of the present invention is: a novel human antimicrobial peptide LL-37 derivative, the amino acid sequence of which is Leu-Leu-Asp-Phe-Lys-Glu-Arg-Lys-Glu-Lys-Gln-Phe-Lys -Arg-Ile-Val-Gln-Arg-Ile-Lys-Asp-Phe-Leu-Arg-Gln-Asn, molecular weight 3177.6D. As shown in Table 1, the novel human antimicrobial peptide LL-37 derivatives include the antibacterial activity structure of the antimicrobial peptide LL-37 Phe-Lys-Arg-Ile-Val-Gln-Arg-Ile-Lys-Asp-Phe- Leu-Arg.

[0016] Peptide name amino acid sequence Antimicrobial peptide LL-37 LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES Antimicrobial Peptide LL-37 Derivatives LLDFKERKEKQFKRIVQRIKDFLRQN

[0017] The antimicrobial peptide LL-37 derivative modified by the scheme of the present invention is rich in glutamine and cysteine, which enhances the stability of the antimicrobial peptide. Adding asparagine and lysine can attract negative charges on the surface of bacteria and enhance antibacteria...

Embodiment 2

[0019] Example 2 of the present invention is: preparation of a novel human antimicrobial peptide LL-37 derivative sodium chloride injection: this embodiment is the preparation of antibacterial drugs for the novel human LL-37 antimicrobial peptide derivative of the present invention The amino acid sequence of the antimicrobial peptide derivative is Leu-Leu-Asp-Phe-Lys-Glu-Arg-Lys-Glu-Lys-Gln-Phe-Lys-Arg-Ile-Val-Gln-Arg-Ile -Lys-Asp-Phe-Leu-Arg-Gln-Asn with a molecular weight of 3177.6D. This antimicrobial peptide derivative is made into polypeptide bacteriocin sodium chloride injection, and every 100ml injection is made up of following components: main agent is the antimicrobial peptide 0.5g of SEQ ID NO:1 sequence, and its percentage is 0.5%, chlorination Sodium 0.9g, the balance is water for injection.

[0020] Weigh the water for injection of 60% of the total volume of the prescribed amount, then add the prescribed amount of SEQ ID NO: 1 sequence antimicrobial peptide deriv...

Embodiment 3

[0021] The third embodiment of the present invention is: the preparation of a novel human-derived antimicrobial peptide LL-37 derivative gel: this embodiment is the application of the novel human-derived LL-37 antimicrobial peptide derivative in the preparation of antibacterial drugs. The amino acid sequence of the antimicrobial peptide derivative is Leu-Leu-Asp-Phe-Lys-Glu-Arg-Lys-Glu-Lys-Gln-Phe-Lys-Arg-Ile-Val-Gln-Arg-Ile-Lys-Asp- Phe-Leu-Arg-Gln-Asn, the molecular weight is 3177.6D. The antimicrobial peptide derivative is made into a polypeptide gel, and every 1000g of the gel is composed of the following components: the antimicrobial peptide 10g of the main drug SEQ ID NO:1 sequence, the auxiliary material Carbomer-940 10g, lauryl azepine Ketone 50ml, glycerin 200ml, Span-85 50ml, triethanolamine 20ml, and the balance is water for injection.

[0022] Weigh 10 g of the excipient Carbomer-940 powder, add an appropriate amount of water for injection and mix well, then add 5...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a novel human antimicrobial peptide LL-37 derivative and its application. The amino acid sequence of the human antimicrobial peptide LL-37 derivative is shown in SEQ ID NO:1. The present invention also includes the application of the above-mentioned novel human antimicrobial peptide LL-37 derivative in the preparation of antibacterial drugs. The antibacterial drug is an antibacterial drug or an antifungal drug, and the bacteria or fungi are at least cryptococcus, histoplasma capsulata, Candida albicans, Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli and Salmonella A sort of. Compared with the prior art, the human antimicrobial peptide LL-37 derivative of the sequence of the present invention has the advantages of strong stability and good antibacterial effect, and has broad application prospects in the field of antibacterial drug preparation.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a novel human antimicrobial peptide LL-37 derivative and its application. Background technique [0002] Antimicrobial peptides are small molecular polypeptides with broad-spectrum antibacterial activity induced by organisms, usually composed of 12-60 amino acids, which are an inherent part of the body's non-specific defense system, and widely exist in bacteria, fungi, and amphibians , insects, higher plants, mammals and even humans. In recent years, due to the abuse of antibiotics leading to bacterial resistance, allergic reactions and environmental pollution, people began to urgently look for new drugs to solve the negative effects of antibiotics. Antimicrobial peptides have the advantages of good thermal stability, broad antibacterial spectrum, no pollution, and no drug resistance. They have become a research and development hotspot at home and abroad, gradually replacing anti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47A61K38/17A61P31/04A61P31/10
CPCA61K38/00A61P31/04A61P31/10C07K14/4723Y02A50/30
Inventor 徐畅李军华谢苍桑张溪之张利鹏姜长安
Owner ZHUHAI INST OF ADVANCED TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap