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Preparation of a superoxide dismutase modified vanadium nitride paste electrode sensor

An electrode sensor and superoxide technology, applied in the field of electrochemical analysis, can solve the problems of low capacity and ineffective use of area, and achieve the effects of wide electrochemical window, low cost and simple preparation method.

Inactive Publication Date: 2019-12-31
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The capacity of single-layer graphene can reach 21μF / cm 2 , but in many cases graphene is stacked with multiple layers, the area between layers is not effectively utilized, and its actual capacity is lower than that of single-layer graphene. Combining graphene with other nanostructures also inhibits the development of graphene layer overlap

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of nano-VN / graphene composite paste electrode: In an agate mortar, add nano-VN: 43g, graphene oxide: 22g, N-ethyl-3-methylpyridine hexafluorophosphate: 15g , liquid paraffin: 10g, petroleum ether: 15.5mL, grind evenly to get nano-VN / graphene / N-ethyl-3-picoline hexafluorophosphate mixture carbon paste; then put the carbon paste into the The inner diameter of the wire is Φ3mm in a glass tube, compacted, dried, polished with polishing powder, polished, and washed with deionized water to obtain a nano-VN / graphene composite paste electrode;

[0029] (2) Preparation of aminopropyltriethoxysilane modified electrode: In the reactor, add absolute ethanol: 96 mL, γ-aminopropyltriethoxysilane: 24g, mix well, and mix nano VN / Put the graphene composite paste electrode in, react at room temperature for 5 hours, raise the temperature to 50±2°C for 6 hours, take out the electrode, wash it with absolute ethanol, dry it, and put it in a glutaraldehyde solution with a mas...

Embodiment 2

[0033] (1) Preparation of nano-VN / graphene composite paste electrode: In an agate mortar, add nano-VN: 45g, graphene oxide: 20g, N-ethyl-3-methylpyridine hexafluorophosphate: 18g , liquid paraffin: 12g, petroleum ether: 9.5mL, grind evenly to get nano-VN / graphene / N-ethyl-3-picoline hexafluorophosphate mixture carbon paste; then put the carbon paste into the The inner diameter of the wire is Φ3mm in a glass tube, compacted, dried, polished with polishing powder, polished, and washed with deionized water to obtain a nano-VN / graphene composite paste electrode;

[0034] (2) Preparation of aminopropyltriethoxysilane modified electrode: In the reactor, add absolute ethanol: 94 mL, γ-aminopropyltriethoxysilane: 26g, mix well, and mix nano VN / Put the graphene composite paste electrode in, react at room temperature for 4 hours, and then raise the temperature to 50±2°C for 6 hours at a constant temperature. Take out the electrode, wash it with absolute ethanol, dry it, and put it in a ...

Embodiment 3

[0038] (1) Preparation of nano-VN / graphene composite paste electrode: In an agate mortar, add nano-VN: 42g, graphene oxide: 25g, N-ethyl-3-methylpyridine hexafluorophosphate: 14g , liquid paraffin: 11g, petroleum ether: 12.5mL, grind evenly to get nano-VN / graphene / N-ethyl-3-picoline hexafluorophosphate mixture carbon paste; then put the carbon paste into the The inner diameter of the wire is Φ3mm in a glass tube, compacted, dried, polished with polishing powder, polished, and washed with deionized water to obtain a nano-VN / graphene composite paste electrode;

[0039] (2) Preparation of aminopropyltriethoxysilane modified electrode: In the reactor, add absolute ethanol: 99 mL, γ-aminopropyltriethoxysilane: 22g, mix well, and mix nano VN / Put the graphene composite paste electrode in, react at room temperature for 6 hours, raise the temperature to 50±2°C for 6 hours, take out the electrode, wash it with absolute ethanol, dry it, and put it in a glutaraldehyde solution with a mas...

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Abstract

The invention discloses a preparation method of a superoxide dismutase modified vanadium nitride paste electrode sensor, characterized by comprising the following steps: firstly preparing a nano VN / graphene compound paste electrode by adopting nano VN, graphene oxide and N-ethyl-3-picoline hexafluorophosphate; then modifying the nano VN / graphene compound paste electrode by adopting gamma-aminopropyl triethoxysilane, so as to obtain an aminopropyl triethoxysilane modified electrode; finally, dissolving bovine serum albumin and superoxide dismutase by adopting a phosphate buffer solution to prepare a superoxide dismutase immobilizing liquid; and then dripping the superoxide dismutase stationary liquid onto the aminopropyl triethoxysilane modified electrode, so as to prepare the superoxidedismutase modified vanadium nitride paste electrode sensor. The electrode has the advantages of being improved in conductivity in comparison with common carbon paste electrodes, wide in electrochemical window, simple in preparation method, low in cost, easy to update in surface, and small in residual current. A detection superoxide radical is high in sensitivity and good in selectivity.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical sensor, in particular to a preparation method and application of a vanadium nitride paste electrode sensor modified by superoxide dismutase. It belongs to the field of electrochemical analysis. Background technique [0002] The enzyme electrode analysis method is to immobilize the enzyme protein molecules by the traditional adsorption method, embedding method, covalent bonding method, and cross-linking method to form an immobilized enzyme film, and then combine with the electrochemical basic electrode to form an enzyme electrode biological A biotechnology in which sensors are used for the analysis of specific substrates. Due to the high specificity of the enzyme, the method has the characteristics of high specificity, good stability, fast detection speed, good selectivity and high sensitivity. Enzyme electrode research started in the 1960s. Since 2000, biosensor technology has been w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/308G01N27/327
Inventor 李慧芝翟玉博陈艳晶
Owner UNIV OF JINAN
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