126 results about "Superoxide radicals" patented technology

This invention discloses a process for preparing a mixed electrolyzed water consisting of a cathodic and an anodic electrolyzed waters comprising the step of electrolyzing an aqueous solution of an organic electrolyte containing a water-soluble inorganic salt in less than 0.1 mM and an organic electrolyte in 1 to 50 mM which is fed into a non-diaphragm electrolytic bath comprising at least a pair of inactive electrodes separated from each other by an inter-electrode distance of 2 mm or less, wherein the aqueous solution of an organic electrolyte with pH equal to that of the mixed electrolyzed water prepared by electrolysis is neutralized with a titration volume less than that for the raw aqueous solution in neutralization titration with an aqueous solution of sodium hydroxide or has a higher dismutation activity to superoxide radical per mole than the raw aqueous solution.

The invention discloses brefeldin A ester derivatives, and preparation and application thereof. The brefeldin A ester derivative compounds have antioxidation activity and antitumor activity, wherein the brefeldin A ester derivative compound disclosed as Formula (I-1), (I-3), (I-5) or (I-7) has favorable antioxidation effect and can remove DPPH, ABTS and super oxygen free radical (O2<->), and the compound (I-7) has better removal effect on DPPH, ABTS and super oxygen free radical (O2<->) than vitamin E; and the compound disclosed as Formula (I-1), (I-3), (I-5) or (I-7) has favorable inhibition activity for human lung cancer cells A549. Therefore, the result shows that the derivatives have wide application prospects in the drug development system, provide a new and broader idea for synthesizing and screening BFA (brefeldin A) derivative drugs and provide a more efficient way for treating related diseases.

The invention discloses an application of a piezoelectric material barium titanate in ultrasonic activation of persulfate for wastewater treatment. Persulfate is activated through the combination of the piezoelectric material and ultrasonic waves, so that sulfate radicals are generated; the sulfate radical free radicals have certain oxidation capacity on pollutants, so that high degradation efficiency is achieved. Compared with a conventional method for promoting persulfate to generate sulfate radicals, the persulfate activation method provided by the invention has obvious advantages; the sulfate radical free radicals generated by piezoelectric activation of the persulfate can form a multiple oxide system with hydroxyl radicals, superoxide radicals, singlet oxygen and the like which are formed in a water body through the piezoelectric effect, so that removal efficiency of the pollutants in the water body is further improved, the removal rate of wastewater of drugs such as ibuprofen andthe like can reach 98% or above, and thus the method can be widely applied to various wastewater treatment systems.

The invention relates to a longan functional drink and a method for preparing the same. The method comprises the following steps of: mixing desired longan pulp with other various raw materials and adding water to the mixture to a certain volume, boiling the mixture until the temperature is in the range of 80-100 DEG C, preserving heat for 20-40 minutes, grinding by using a pulp refiner until the particle granularity is about 15-20 microns, and filtering to obtain longan mixed juice; inoculating the longan mixed juice with sbacillus containing a manganese superoxide dismutase gene for fermentation culture; and keeping in cold storage, thereby obtaining the longan functional drink. The obtained longan functional drink is advantageous for improving the digestion absorption efficiency of the human body to food, improving the feeding of satiety after eating, reducing the possibility of abdominal distension exhaust, promoting normal defecation, reducing the pressure of the gastrointestinal tract, promoting vigor, promoting the health of the circulatory system, helping the body to adjust operation of the immunologic function and enhancing the human body defense mechanism; besides, the drink is advantageous for clearing away superoxide radicals generated in the human body and protecting the cells against damage, and has the characteristics of resisting aging and radiation, improving the tolerance of the cells to oxygen emergency reaction and the like.

The invention provides a preparation method of an antibacterial silver molybdate/MIL-101 (Fe, Zn) heterojunction photocatalytic membrane and a product and application thereof. According to the preparation method, a heterojunction formed by compounding an inorganic antibacterial agent silver molybdate with broad-spectrum property and a bimetal organic framework compound MIL-101 (FE, Zn) is preparedinto the composite photocatalytic membrane by adopting an electrostatic spinning method. The product and use thereof are disclosed. The large specific surface area and rich active sites of MIL-100 (Fe) are fully utilized, interface charge transfer is effectively promoted, the separation capacity of photo-induced electrons and holes is improved, and adsorption and photocatalytic degradation removal of organic pollutants on the surface of the catalyst are promoted. Meanwhile, the surface of the silver molybdate is excited by illumination to generate superoxide free radicals and hydroxyl free radicals, so that the purposes of sterilization and bacteriostasis are achieved, the water treatment efficiency is effectively improved, the membrane pollution is reduced, the double-effect purificationof water quality is realized, the application prospect is relatively great, and a feasible scheme is provided for water pollution treatment.

Cerium oxide nanoparticles (CeONP) can be used to treat or prevent neurodegenerative diseases, including for example Alzheimer's Disease, Parkinson's Disease, Huntington's Disease, AIDS-related dementia, ALS, progressive supranuclear palsy, and encephalitis, as well as mitochondrial diseases and diseases associated with mitochondrial damage. In particular, CeONP having an average size of about 2 nm to about 100 nm can be administered in an amount sufficient to block production of hydroxyl or superoxide radicals, block free radical production by Aβ_(1-42), block Aβ_(1-42)-induced neuronal death, block Aβ_(1-42)-induced [Ca²⁺]_i dysfunction in neurons, block Aβ_(1-42)-induced lipid peroxidation, decrease loss of dopaminergic neurotransmission, or reduce mitochondrial dysfunction in a cell. CeONP can also be effective in treating conditions involving toxic exposures to compounds that induce mitochondrial dysfunction, such as rotenone, cyanide, carbon monoxide, polychlorinated biphenyls (PCBs) and other mitochondrial toxins.

Hemicelluloses may be extracted by alkaline peroxide treatment without removal of ferulate residues. Hemicelluloses prepared in this way retain their ability to gel. Such gelling hemicelluloses may be extracted with a number of different oxidizing species including hydrogen peroxide anion, hydrogen peroxide radical, hydroxyl radical, superoxide radical and oxide radical. A process has thus been developed for producing a hemicellulose gel that may be carried out at cold temperatures, thus facilitating enzymic co-processing, and that is high yielding and produces a gel that is light in color.

The invention discloses a preparation method of a superoxide dismutase modified vanadium nitride paste electrode sensor, characterized by comprising the following steps: firstly preparing a nano VN/ graphene compound paste electrode by adopting nano VN, graphene oxide and N-ethyl-3-picoline hexafluorophosphate; then modifying the nano VN/ graphene compound paste electrode by adopting gamma-aminopropyl triethoxysilane, so as to obtain an aminopropyl triethoxysilane modified electrode; finally, dissolving bovine serum albumin and superoxide dismutase by adopting a phosphate buffer solution to prepare a superoxide dismutase immobilizing liquid; and then dripping the superoxide dismutase stationary liquid onto the aminopropyl triethoxysilane modified electrode, so as to prepare the superoxidedismutase modified vanadium nitride paste electrode sensor. The electrode has the advantages of being improved in conductivity in comparison with common carbon paste electrodes, wide in electrochemical window, simple in preparation method, low in cost, easy to update in surface, and small in residual current. A detection superoxide radical is high in sensitivity and good in selectivity.

The present invention discloses a device for improving the action of the head, which sprays the air current produced an air current producer to the head and comprises a box, an air inlet arranged on one side of the box; an air current producer comprising a ceramic plate unit for producing hydrogen ions and an electron generation unit spaced from the ceramic plate unit for generating electrons and superoxide radicals; and a spraying opening for spraying the superoxide radicals produced by the air current producer and hydrogen atoms to the head. The present invention further discloses a device for preventing skin from aging by using a hydrogen producer, comprising: a box; an air inlet arranged on one side of the box; a hydrogen producer for producing hydrogen atoms; and a spraying opening for spraying the hydrogen atoms produced by the hydrogen producer to the skin, wherein the hydrogen producer comprises a ceramic plate unit for producing hydrogen ions and an electron generation unit spaced from the ceramic plate unit for generating electrons.

Reduction of adverse inflammatory reaction to an implant or a transplant, or following trauma or infection, is achieved through catalysis of dismutation of the superoxide radical anion by an osmium containing compound. Treatment diseases caused by superoxide dismutase deficiency or mutation with superoxide radical anion dismutating osmium compounds or a carbonate radical anion decay catalyzing polymeric N-oxide is also disclosed.

An electromagnetic field generating apparatus for treating pathological cells, in particular with tumor suppression function. The ratio of static magnetic field to alternating magnetic field is in the range of 0.5-2.5, and the total intensity is in the range up to 100 mT. It provides non-invasive low toxicity and side effects, significantly inhibiting a plurality of tumors, influencing the intracellular superoxide radical content, and promoting cell autophagy. The mechanism of action of the magnetic field is on the electron spin energy levels and consequently on the free radicals concentration. Additional magnetic field having frequency up to 100 MHz and intensity down to microTesla range can be also used to improve the antitumor efficacy in combination with the static as well as the extremely low frequency electromagnetic field (1-300 Hz) influencing the spin hyperfine resonance. A combination method is also provided of a power-frequency electromagnetic field generating device and a platinum-based medicine. The in vitro and in vivo experiments confirmed that the tumor therapeutic electromagnetic field combined with platinum-based chemotherapy drugs can enhance the efficacy of platinum-based chemotherapy drugs and magnetic field alone, a significant inhibition of a variety of tumors; can reduce the dose of cisplatin and reduce side effects.

The invention relates to a preparation method of a multivalent nanometer manganese oxide for effectively removing organic pollutants in water. The method comprises the following steps: preparing a medium by using yeast extract powder, acid hydrolyzed casein, glucose, HEPES, CaCl2, MgSO4, FeCl3 and trace elements (CuSO4, ZnSO4, CoCl2 and NaMoO4), adjusting the pH value to 6-8 by using 3M of NaOH, carrying out high temperature and high pressure disinfection, inoculating Pseudomonas sp.G7 according to 1%(V/V), carrying out shaking culture in a 20-30DEG C constant temperature culturing box for 20-30h, adding 0.3-0.5mM of a filtered and disinfected aqueous solution of MnCl2, continuously shaking for 12-15d, and washing the obtained precipitate with deionized water to obtain the multivalent nanometer manganese oxide. The above material has very good catalytic activity and stability, 2-chlorophenol converts to formic acid, acetic acid, oxalic acid and other micro-molecular organic acids in the degradation process, and the generation of a superoxide radical by the multivalent activated molecule oxygen of manganese is the main reason causing efficient degradation.

The invention relates to a high-temperature drought stress resistance agent and application of the agent and belongs to the field of crop planting. In order to overcome the technical defects that thestress resistance effect is poor by applying glycine betaine alone in the prior art and corresponding substitute products fall short in the market, the provided high-temperature drought stress resistance agent is prepared from glycine betaine, urea and Tween-20. When the agent is applied to annual seedlings or annual or biennial or triennial plants, drop of the chlorophyll content under an adversesituation can all be alleviated, and the photosynthetic capacity of leaves is improved. Accumulation of superoxide radicals (O2 -) in the leaves is reduced, the relative electrolytic leakage and membrane lipid peroxidation level of the leaves are lowered, plant resistance to drought and other stress is improved, and the agent is suitable for application and popularization in the agriculture.

The invention discloses a method for fermenting a red raspberry and grape enzyme by a composite strain. The method comprises cleaning red raspberries and grapes through sterile water under aseptic conditions, adding sterile water into a juicer, carrying out juicing, carrying out ultra-high temperature instantaneous sterilization on the mixed liquid, adding yeast and lactic acid bacteria into the mixed liquid subjected to sterilization, carrying out fermentation for five weeks, taking out the fermented enzyme supernatant, carrying out blending, adding sterile white granulated sugar syrup and honey into the supernatant and carrying out homogenization to obtain the red raspberry and grape enzyme. The red raspberries and grapes contain polyphenols and flavonoids and have oxidization resistance. The total phenol content, DPPH free radical scavenging ability, superoxide radical scavenging ability and ABTS radical scavenging ability in the fermentation process are gradually increased so thatantioxidant activity is significantly increased compared than that before fermentation. The raspberry and grape enzyme has good antioxidant properties.

The invention discloses a preparation method of an organic pollutant photolysis catalyst. The preparation method is characterized by comprising steps as follows: regular ceramic is taken as carriers, are subjected to array hole formation with laser and activating treatment with potassium permanganate and then ultrasonically load sol-gel containing BiVO4, TiO2 and graphene, a product is subjected to vacuum drying and is subjected to high-temperature calcination finally, and industrial organic pollutant photolysis catalyst based on BiVO4-TiO2-graphene loaded regular ceramic is prepared. The photolysis catalyst can simultaneously release molecular radicals through plasma discharge and produce superoxide radicals and hydroxyl radicals through photocatalysis under radiation of a plasma emission (UV) light, thereby efficiently and thoroughly degrading toxic, harmful and stink industrial waste gas and industrial organic pollutants containing sulfur, nitrogen, ammonia, phenol, PAHs (polycyclic aromatic hydrocarbons), pesticides, antibiotics, viruses, bacteria and the like.

The invention belongs to the technical field of natural polymers and discloses a mallotus furetianus homopolysaccharide with antioxidant activity and alpha-glucosidase inhibition activity as well as apreparation method and application thereof. The mallotus furetianus homopolysaccharide disclosed by the invention is named as MFP-2A, and the structural formula of the mallotus furetianus homopolysaccharide is as shown in the specification. The mallotus furetianus homopolysaccharide MFP-2A in the invention is an acid polysaccharide, simultaneously contains six glucosidic bonds of -4,6)-Galp-(1-,-3,6)-Manp-(1-,-5)-Araf-(1-,T-Araf-(1-,-4)-GalpA-(1- and -4)-Glc-(1-, particularly contains alpha-(1,4) glucosidic bonds, has high-efficiency abilities of scavenging DPPH (diphenyl-1-picrylhydrazyl) free radicals, ABTS (azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) free radicals and superoxide radicals, also has properties of resisting oxidation and inhibiting alpha-glucosidaseactivity, can serve as a natural antioxidant, and is applied to preparation of hypoglycemic drugs or health foods.

The invention puts forward a Nereis succinea antioxidant peptide and a preparation method and application thereof. The preparation method of the invention comprises the following steps: 1) taking liveNereis succinea, conditioning and pulping to obtain a serous fluid; 2) adding 600-1000 U/g of pepsase into the serous fluid, carrying out enzymolysis at 40-45 DEG C for 1-4 h, carrying out enzyme deactivation at 90-100 DEG C for 4-6 min, and cooling to obtain an enzymatic hydrolysate; and 3) carrying out coarse filtration on enzymatic hydrolysate, and carrying out fine filtration, ultrafiltration, concentration, desalination, purification and drying to obtain the Nereis succinea antioxidant peptide. The preparation method of the invention is simple, is convenient to operate, is green and environment-friendly, and is easy to realize industrialization. The Nereis succinea antioxidant peptide has high purity, strong activity and good antioxidant effect, has scavenging effect on DPPH free radicals, superoxide free radicals and hydroxyl free radicals, and has an obvious protective effect on oxidative damage of HUVEC cells. The Nereis succinea antioxidant peptide can be widely used in the production of nutritional food, functional food and feeds.

The invention relates to the technical field of photocatalytic degradation of organic pollutants, and discloses a carbon nanotube loaded CdSe-g-C3N4 photocatalytic material and a preparation method thereof. The photocatalytic material comprises the following formula raw materials: cadmium chloride, sodium selenosulfate, urea, melamine and carbon nanotubes. According to the material and the preparation method thereof, the formation interface of CdSe and g-C3N4 has an overlapped energy band heterostructure with good matching performance; the heterostructure can effectively separate photo-generated carriers and transmit photo-generated charges, and the effective separation of photo-induced electrons and holes is enhanced, and therefore, a large number of freely moving photo-induced electronse<-> and holes h<+> are generated; the holes h<+> have very strong oxidability, the photo-induced electrons e<-> and dissolved oxygen in water can generate superoxide free radical O<2->., holes h<+> and superoxide free radical O<2->. have very high oxidation-reduction potentials and can be subjected to an oxidation-reduction reaction with various organic pollutants, so that the degradation efficiency of the photocatalytic material on organic dye pollutants is greatly improved.

The invention discloses recombinant human-like elastin and a composition thereof. The recombinant human-like elastin is formed through series connection of short repeat amino acid sequences, wherein the short repeat amino acid sequences are target protein polypeptide sequences which are formed through 3-7 times of tandem repeat of (VAPGVG)3S, and the target protein polypeptide sequences are shownin SEQ No. 1. The recombinant human-like elastin which is obtained through in vitro expression of a prokaryotic expression system has high activity for removal of superoxide radicals. Good resistanceto degradation is still maintained through a small number of tandem repeat of the active peptide fragments, and the catalytic activity can be kept longer than the catalytic activity of a single original fragment; and the service cycle of the recombinant human-like elastin can be improved significantly through assistance of a selected protective agent.

The invention discloses a composite nano material for air purification and a preparation method thereof. The composite nano material is prepared from the following raw materials in parts by weight: 25 to 50 parts of composite nano particles, 15 to 30 parts of adsorption particles, 3 to 5 parts of hydroxyethyl methyl cellulose, 3 to 5 parts of lignocellulose and 10 to 15 parts of biomass glue. The preparation method comprises the following steps: mixing hydroxyethyl methyl cellulose, lignocellulose and biomass glue, then carrying out hydrolytic condensation to prepare sol, sequentially adding composite nano particles and adsorption particles, carrying out uniform-speed stirring for 30 min, then carrying out evaporation and dehydration to prepare gel, and then carrying out drying and heat treatment to prepare the composite nano-material for air purification. When the composite nano particles are used, visible light can excite indium vanadate to generate electrons, the electrons are transferred to the surfaces of the composite nano particles, the electrons and oxygen molecules adsorbed on the surfaces are combined to form superoxide free radicals, then the superoxide free radicals and organic pollutants adsorbed on the surfaces of the composite nano particles are subjected to an oxidation-reduction reaction, and the air purification effect of the composite nano particles is further improved.

The invention discloses an implant abutment gum-penetrating structure having a visible light functionalization function and a production method thereof. The structure comprises an implant abutment, amaterial of the implant abutment is titanium, the implant abutment is composed of an abutment upper part, the abutment gum-penetrating structure, and an abutment lower part, the abutment gum-penetrating structure is positioned between the abutment upper part and the abutment lower part, the surface of the abutment gum-penetrating structure is the nano structure having a nano-gold particles-loadedTiO2 nanotube, and the structure has the characteristic of generating light functionalization under irradiation of visible light. The abutment gum-penetrating structure is used for preparing the nano-gold particles-loaded TiO2 nanotube through an anodization method and a self assembly method. The abutment gum-penetrating structure is capable of generating light functionalization under irradiationof the visible light, the generated superoxide radical and hydroxyl radical to kill peri-implantitis pathogenic bacteria, destroys a plaque biofilm, purifies the surface of the abutment gum-penetrating structure, and is used for preventing and treating peri-implantitis.

The present invention encompasses methods and compositions for detecting pathogenic bacteria. Additionally, the present invention encompasses methods and compositions for catalyzing the dismutation of superoxide radicals.

The invention discloses a support material with conductivity and an antioxygenic property and a preparation method thereof. The support material is prepared from phenylamine pentamer modified by glutathione, and gelatin. The support material has favorable mechanical strength and adjustable degradation characteristic. More importantly, the support material has good conductivity, and can effectively scavenge various oxygen radicals such as hydroxyl radicals, organic oxygen radicals and superoxide radicals. According to the support material with the conductivity and the antioxygenic property and the preparation method thereof provided by the invention, the dual-network conductive support material is prepared by adopting one-step shaping, so that the preparation method is simple, components are uniform and controllable. More importantly, the support material has favorable biocompatibility and can support the adhesion and the growth of various cells. Stem cells can be promoted to differentiate into cardiac muscle cells, so that the support material has potential application prospect in cardiac muscle tissue engineering.