Room temperature perfusion liquid for isolated heart preservation

A perfusate and heart technology, applied in the field of medicine and biology, can solve the problem that the heart cannot be stored for a long time and does not contain red blood cells, so as to solve the problem of insufficient source of heart donors, prevent ischemia-reperfusion injury, good market prospects and social welfare. effect of value

Active Publication Date: 2018-11-30
嘉兴莱普晟医疗科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem that the heart cannot be stored for a long time in the prior art, the present invention provides a room temperature perfusate for heart preservation, which is characterized in that the heart perfusate contains substances and biological enzymes for removing free radicals, and Does not contain red blood cells

Method used

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  • Room temperature perfusion liquid for isolated heart preservation
  • Room temperature perfusion liquid for isolated heart preservation
  • Room temperature perfusion liquid for isolated heart preservation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 The composition and connection method of the mechanical perfusion system for heart preservation

[0026] Such as figure 1 As shown, the central processing unit module 1 is connected to the peristaltic pump 7, and the output power of the peristaltic pump is controlled by the control module 20. Further, the peristaltic pump 7 is connected to the cardiac perfusate storage 6, and the cardiac perfusate storage 6 is connected to the cardiac storage via the input conduit 14. The heart storage device 8 is connected to the heart storage device 8, and an opening is provided in the heart storage device 8, and the input catheter 14 can enter the inside of the heart storage device and be connected with the heart preserved in vitro. The peristaltic pump 7 controls the perfusion pressure and flow rate of the perfusate through the change of the output power. The inner wall of the cardiac perfusate reservoir is provided with a cold and heat regulating tube, and the cold and...

Embodiment 2

[0027] Example 2 Procurement and perfusion of donor heart

[0028] 1 experimental animal

[0029] 6-10 male Chinese miniature pigs, weighing 30±3Kg. All experimental animals were treated humanely and complied with the "Guidelines for the Management and Use of Experimental Animals" promulgated by the National Institutes of Health of the United States, and the experimental protocol was approved by the relevant experimental animal ethics committee.

[0030] 2. Method

[0031] (1) Experimental animals were fasted for 8 hours before operation. Anesthesia was induced by intramuscular injection of sodium pentobarbital, and body weight was weighed and recorded. The limbs were immobilized, and the right marginal ear vein access was established. Open the upper and lower jaws to expose the throat, use a laryngoscope to provoke the epipharynx, insert a tracheal tube, and connect a ventilator for assisted breathing. The tidal volume is maintained at 15ml / kg, the respiratory rate is mai...

Embodiment 3

[0037] Example 3 Effect of Itoside on Mechanical Perfusion Preserved Heart in Vitro

[0038] Itoside can scavenge free radicals, inhibit lipid peroxidation, and can prevent and repair damage to cells caused by hypoxia, ischemia or reperfusion. In order to explore the effect of iatonin on mechanically perfused isolated hearts, two different cardiac perfusates were designed in this example, and the specific components are as described in Table 1:

[0039] Table 1 Composition of perfusate A and perfusate B (1L)

[0040]

[0041]

[0042] 1. Perfusion method

[0043] 6 male Chinese miniature pigs, weighing 30±3Kg. Randomly divided into two groups, group A and group B, with 3 rats in each group. Group A was perfused with perfusate A, and group B was perfused with perfusate B. For the acquisition and perfusion of the donor heart, refer to Example 2. The donor heart was mechanically perfused for 8 hours in vitro.

[0044] 2. Detection method of heart state

[0045] 2.1 Det...

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Abstract

The present invention provides a room temperature perfusion liquid for isolated heart preservation, wherein per L of the perfusion liquid contains 4.5 mg of itoside, 3 g of hydroxy starch, 7.5 g of adenosine triphosphate, 40 g of perfluorocarbon, 4.5 g of lecithin, 1.25 mg of galactosidase, 1 mg of acetylgalactosidase, 2.5 mg of diazoxide, 550 mg of histidine, 210 mg of tryptophan, 170 mg of glutamine, 625 mg of arginine, 3 g of penicillin, 15 mmol sodium chloride, 10 mmol potassium chloride, 5 mmol magnesium chloride, 7 mmol NaHCO3, 1.25 mmol CaCl2, 7 mg of coenzyme Q10, and 6 mg of vitamin B12. According to the present invention, the room temperature perfusion liquid can repair the damage of myocardial cells caused by ischemia re-injury, reduce the immunogenicity of the transplanted heart, and improve the utilization rate of the donor heart.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to a normal-temperature perfusate used for preservation of isolated hearts. Background technique [0002] After four decades of research in treating patients with end-stage heart failure, artificial heart valves, ventricular assist devices, and the latest stem cell therapy are still not enough to replace heart transplants. Heart transplantation has been widely accepted as a treatment for end-stage heart disease, and the effect of transplantation is closely related to the health status of the donor and recipient, organ preservation conditions, immune technology, and postoperative treatment. Among them, a high-quality heart Preservation is one of the keys to improve the success rate and long-term survival rate of delayed heart transplantation. [0003] Organ preservation technology has made great progress. Kidneys, livers, and pancreas can generally last for ...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0221A01N1/0226
Inventor 李建辉杨子江郑俊
Owner 嘉兴莱普晟医疗科技有限公司
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