Polymer thermosensitive liposome loaded with yeast glucan and carnosic acid

A yeast glucan and heat-sensitive liposome technology, applied in the field of traditional Chinese medicine, can solve the problems of unseen and unseen yeast glucan and carnosic acid compositions and application reports of novel nanostructure carriers, etc. Good slow release performance, good slow release effect, low permeability effect

Inactive Publication Date: 2018-11-30
GUANGZHOU JIAYUAN PHARMA TECH
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Chinese invention patent CN201310162941.0 discloses "oil-in-water yeast β-glucan nanoemulsion adjuvant and its preparation method and application". The adjuvant in this invention mainly includes 0.2-3% water-soluble yeast glucan, white oil 4-18%, surfactant 6-25%, co-surfactant 4-15%, and distilled water for injection as the balance, used in the preparation of vaccines; Chinese invention patent CN201510120866.0 discloses "Carnosic Acid Treatment I Uses of Type I Diabetes”, the invention relates to the use of carnosic acid in the preparation of compositions for the treatment or prevention of Type I diabetes; no yeast glucan and carnosic acid composition has been prepared into a new nanostructure carrier, no yeast glucan Application report of sugar and carnosic acid composition and its new nanostructure carrier in reversing tumor multidrug resistance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polymer thermosensitive liposome loaded with yeast glucan and carnosic acid
  • Polymer thermosensitive liposome loaded with yeast glucan and carnosic acid
  • Polymer thermosensitive liposome loaded with yeast glucan and carnosic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Polymeric thermosensitive liposome loaded with yeast glucan and carnosic acid

[0027] (1) The preparation method is as follows:

[0028] 1) Add 7mg of polylysine, 50mg of phospholipids (25mg of dipalmitoylphosphatidylcholine, 25mg of dipalmitoylphosphatidylcholine), and 3mg of tocopherol to 50ml of absolute ethanol at the same time, and stir evenly to form a membrane material solution;

[0029] 2) While stirring, slowly add 8mg of carnosic acid and 100mg of cholesterol into the membrane material solution, mix well and rotate under reduced pressure until the lipid film is formed;

[0030] 3) Fully dissolve 10 mg of yeast glucan in 20 ml of PBS buffer solution with a pH of 7.4 as the water phase;

[0031] 4) Put the lipid film into the water phase, shake it well, and repeatedly freeze-thaw 6 times at -20°C and 30°C, and then pass the initially obtained liposomes through 100nm polycarbonate under the condition of ice bath Membrane extrusion, filter sterilizati...

Embodiment 2

[0059] Example 2 Polymer thermosensitive liposome loaded with yeast glucan and carnosic acid

[0060] The preparation method is as follows:

[0061] 1) Add 10mg of polylysine, 80mg of phospholipids (40mg of dipalmitoylphosphatidyl, 40mg of heptadecanoic acid phospholipids), and 3mg of tocopherol into 50ml of absolute ethanol at the same time, and stir evenly to form a membrane material solution;

[0062] 2) While stirring, slowly add 5mg of carnosic acid and 160mg of cholesterol into the membrane material solution, mix well and rotate under reduced pressure until the lipid film is formed;

[0063] 3) Fully dissolve 5 mg of yeast glucan in 20 ml of PBS buffer solution with a pH of 7.4 as the water phase;

[0064] 4) Put the lipid film into the water phase, shake it well, and repeatedly freeze-thaw 6 times at -20°C and 30°C, and then pass the initially obtained liposomes through 100nm polycarbonate under the condition of ice bath Membrane extrusion, filter sterilization, to ob...

Embodiment 3

[0066] Example 3 Polymeric thermosensitive liposome loaded with yeast glucan and carnosic acid

[0067] The preparation method is as follows:

[0068] 1) Add 10mg of polylysine, 50mg of phospholipids (25mg of dipalmitoylphosphatidylcholine, 25mg of distearoylphosphatidylcholine ethanolamine), and 2mg of tocopherol into 50ml of absolute ethanol at the same time, stir evenly, and form a film material solution;

[0069] 2) While stirring, slowly add 10mg of carnosic acid and 100mg of cholesterol into the membrane material solution, mix well and rotate under reduced pressure until the lipid film is formed;

[0070] 3) Fully dissolve 15 mg of yeast glucan in 20 ml of PBS buffer solution with a pH of 7.4 as the water phase;

[0071] 4) Put the lipid film into the water phase, shake it well, and repeatedly freeze-thaw 6 times at -20°C and 30°C, and then pass the initially obtained liposomes through 100nm polycarbonate under the condition of ice bath Membrane extrusion, filter ster...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
polydispersity indexaaaaaaaaaa
encapsulation rateaaaaaaaaaa
Login to view more

Abstract

The invention relates to a polymer thermosensitive liposome loaded with yeast glucan and carnosic acid and a preparation method thereof. The polymer thermosensitive liposome comprises the following raw materials by weight: 5-15 parts of the yeast glucan, 5-10 parts of the carnosic acid, 4-10 parts of polylysine, 25-80 parts of phospholipid, 2-5 parts of tocopherol and 50-160 parts of cholesterol.The two drugs are respectively encapsulated in different positions of a lipid bilayer by membrane dispersion technology, and prescription technology and physicochemical properties are optimized and investigated to prepare a nanostructured carrier with excellent properties, and the polymer thermosensitive liposome can reverse multidrug resistance of cancer cells.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to a polymer thermosensitive liposome loaded with yeast glucan and carnosic acid and a preparation method thereof. Background technique [0002] In recent years, there have been more and more studies on tumor multidrug resistance. The final reason is that the self-protection mechanism of tumor cells uses its own transporter efflux to pump various chemotherapeutic drugs out of tumor cells, resulting in effective The reduction of chemotherapy doses cannot achieve the desired therapeutic effect, so the research and development of drugs to reverse tumor multidrug resistance is as important as the development of new chemotherapy drugs. [0003] Yeast glucan is a kind of immune polysaccharide, which participates in various life regulation of biological cells, and has low toxic and side effects. In recent years, studies have shown that yeast glucan also has the func...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/192A61K31/716A61K47/22A61K47/34A61P35/00
CPCA61K9/1271A61K31/192A61K31/716A61K47/22A61K47/34A61P35/00A61K2300/00
Inventor 王冰月王琦白莉
Owner GUANGZHOU JIAYUAN PHARMA TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap