Polymer thermosensitive liposome loaded with yeast glucan and carnosic acid
A yeast glucan and heat-sensitive liposome technology, applied in the field of traditional Chinese medicine, can solve the problems of unseen and unseen yeast glucan and carnosic acid compositions and application reports of novel nanostructure carriers, etc. Good slow release performance, good slow release effect, low permeability effect
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Embodiment 1
[0026] Example 1 Polymeric thermosensitive liposome loaded with yeast glucan and carnosic acid
[0027] (1) The preparation method is as follows:
[0028] 1) Add 7mg of polylysine, 50mg of phospholipids (25mg of dipalmitoylphosphatidylcholine, 25mg of dipalmitoylphosphatidylcholine), and 3mg of tocopherol to 50ml of absolute ethanol at the same time, and stir evenly to form a membrane material solution;
[0029] 2) While stirring, slowly add 8mg of carnosic acid and 100mg of cholesterol into the membrane material solution, mix well and rotate under reduced pressure until the lipid film is formed;
[0030] 3) Fully dissolve 10 mg of yeast glucan in 20 ml of PBS buffer solution with a pH of 7.4 as the water phase;
[0031] 4) Put the lipid film into the water phase, shake it well, and repeatedly freeze-thaw 6 times at -20°C and 30°C, and then pass the initially obtained liposomes through 100nm polycarbonate under the condition of ice bath Membrane extrusion, filter sterilizati...
Embodiment 2
[0059] Example 2 Polymer thermosensitive liposome loaded with yeast glucan and carnosic acid
[0060] The preparation method is as follows:
[0061] 1) Add 10mg of polylysine, 80mg of phospholipids (40mg of dipalmitoylphosphatidyl, 40mg of heptadecanoic acid phospholipids), and 3mg of tocopherol into 50ml of absolute ethanol at the same time, and stir evenly to form a membrane material solution;
[0062] 2) While stirring, slowly add 5mg of carnosic acid and 160mg of cholesterol into the membrane material solution, mix well and rotate under reduced pressure until the lipid film is formed;
[0063] 3) Fully dissolve 5 mg of yeast glucan in 20 ml of PBS buffer solution with a pH of 7.4 as the water phase;
[0064] 4) Put the lipid film into the water phase, shake it well, and repeatedly freeze-thaw 6 times at -20°C and 30°C, and then pass the initially obtained liposomes through 100nm polycarbonate under the condition of ice bath Membrane extrusion, filter sterilization, to ob...
Embodiment 3
[0066] Example 3 Polymeric thermosensitive liposome loaded with yeast glucan and carnosic acid
[0067] The preparation method is as follows:
[0068] 1) Add 10mg of polylysine, 50mg of phospholipids (25mg of dipalmitoylphosphatidylcholine, 25mg of distearoylphosphatidylcholine ethanolamine), and 2mg of tocopherol into 50ml of absolute ethanol at the same time, stir evenly, and form a film material solution;
[0069] 2) While stirring, slowly add 10mg of carnosic acid and 100mg of cholesterol into the membrane material solution, mix well and rotate under reduced pressure until the lipid film is formed;
[0070] 3) Fully dissolve 15 mg of yeast glucan in 20 ml of PBS buffer solution with a pH of 7.4 as the water phase;
[0071] 4) Put the lipid film into the water phase, shake it well, and repeatedly freeze-thaw 6 times at -20°C and 30°C, and then pass the initially obtained liposomes through 100nm polycarbonate under the condition of ice bath Membrane extrusion, filter ster...
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