Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Monascus culture method capable of achieving high-yield lovastatin

A technology of lovastatin and culture method, applied in the field of microorganisms, can solve the problems of low lovastatin yield, achieve high promotion and application value, increase biomass, and simple operation

Active Publication Date: 2018-11-30
YANGTZE UNIVERSITY
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to overcome above-mentioned technical deficiency, propose a kind of cultivation method of Monascus high-yield lovastatin, solve the technical problem that the lovastatin output of existing Monascus is lower

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Monascus culture method capable of achieving high-yield lovastatin
  • Monascus culture method capable of achieving high-yield lovastatin
  • Monascus culture method capable of achieving high-yield lovastatin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The present embodiment provides a kind of cultivation method of Monascus high-yield lovastatin, comprising the steps:

[0024] (1) Cultivation of Monascus species: Dilute an appropriate amount of Monascus species with sterile water to 10 -6 times, take and dilute to 10 -6 2 times the bacterial solution evenly spread on the CYA medium, and then put the CYA medium into a constant temperature incubator, cultivate at 30°C for 12 days to activate the strains, observe the appearance of the colonies, and select the strains with good growth and high vitality. Rinse the selected strains with sterile water and scrape them off, pour the liquid containing the strains into a sterile funnel with four layers of sterilized lens-cleaning paper to filter, and inject the filtered bacterial liquid into the sterile funnel 1. In a 50mL Erlenmeyer flask equipped with small glass beads, repeat 3 times to wash all the spores on the culture medium, count them under a microscope, and dilute the ...

Embodiment 2

[0033] This example provides a method for cultivating high-yielding lovastatin from Monascus. The specific cultivating method is the same as in Example 1, except that the amount of salicylic acid per liter of PDB liquid medium is 30 μmol.

[0034] Adopt the method identical with embodiment 1 to measure the concentration of lovastatin in the fermented liquid, the output of the lovastatin that Monascus produces in the present embodiment is 6.57 μ g / mL.

Embodiment 3

[0036] This example provides a method for cultivating high-yield lovastatin from Monascus. The specific method is the same as in Example 1, except that the amount of salicylic acid per liter of PDB liquid medium is 100 μmol.

[0037] Adopt the method identical with embodiment 1 to measure the concentration of lovastatin in the fermented liquid, the output of the lovastatin that Monascus produces in the present embodiment is 7.519 μ g / mL.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Monascus culture method capable of achieving high-yield lovastatin. The Monascus culture method comprises: (1) diluting and oscillating an activated Monascus strain to obtaina single spore suspension, inoculating the single spore suspension into a PDB liquid culture medium, and culturing for 12 days at a temperature of 30 DEG C to obtain a Monascus suspension; and (2) adding salicylic acid or methyl jasmonate to a PDB liquid culture medium, inoculating the Monascus suspension into the PDB liquid culture medium, and carrying out shaker culture for 12 days at a temperature of 30 DEG C under a rotation speed of 180 r / min. According to the present invention, salicylic acid or methyl jasmonate is added to the specific culture medium, and the Monascus is cultured underthe specific conditions, such that the biomass of the Monascus is effectively increased by promoting the growth of Monascus hyphae or regulating the metabolic process of Monascus so as to effectivelyimprove the Monascus metabolism and the yield of lovastatin; and the method has characteristics of high efficiency, short time consumption, simple operation, low cost and high promotion and application value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for cultivating high-yield lovastatin by Monascus. Background technique [0002] Currently, there are four main types of lipid-lowering drugs: resins, niacin, fibrates, and statins, among which statins represented by lovastatin are currently the most important class of lipid-lowering drugs. Lovastatin is a competitive inhibitor of the rate-limiting enzyme hydroxymethylglutaryl coenzyme A (HMG-CoA) reductase in endogenous cholesterol synthesis. It has been clinically verified that lovastatin is highly effective, safe and low-toxic A good drug for the treatment of hyperlipidemia. Since U.S. Merck Company developed the lovastatin series and launched a new generation of cholesterol-lowering drugs in 1987, lovastatin has been popular among patients as a new type of blood lipid regulator. [0003] At present, the production strains of lovastatin are mainly...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P17/06C12R1/645
CPCC12P17/06
Inventor 高梦祥张佳兰
Owner YANGTZE UNIVERSITY
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com