Monascus culture method capable of achieving high-yield lovastatin

A technology of lovastatin and culture method, applied in the field of microorganisms, can solve the problems of low lovastatin yield, achieve high promotion and application value, increase biomass, and simple operation

Active Publication Date: 2018-11-30
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to overcome above-mentioned technical deficiency, propose a kind of cultivation

Method used

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  • Monascus culture method capable of achieving high-yield lovastatin
  • Monascus culture method capable of achieving high-yield lovastatin
  • Monascus culture method capable of achieving high-yield lovastatin

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1:

[0023] This embodiment provides a method for culturing high-yield lovastatin of Monascus, including the following steps:

[0024] (1) Cultivation of Monascus species: Dilute an appropriate amount of Monascus species with sterile water to 10 -6 Times, dilute to 10 -6 Spread the bacteria solution evenly on the CYA medium, then put the CYA medium in a constant temperature incubator, and cultivate at 30°C for 12 days to activate the strains, observe the appearance of the colony, and select the strains with good growth and high viability. Rinse the selected strains with sterile water and scrape them off, pour the liquid with the strains into a sterile funnel with four layers of sterilized lens cleaning paper, and inject the filtered bacterial liquid into the sterile funnel. 、In a 50mL Erlenmeyer flask containing small glass beads, repeat 3 times to rinse all the spores on the culture medium, count by microscopy, and dilute the strain to 10 with sterile water -6 Then...

Example Embodiment

[0032] Example 2:

[0033] This example provides a method for culturing Monascus with high-yield lovastatin. The specific culturing method is the same as that of Example 1, except that the amount of salicylic acid per liter of PDB liquid medium is 30 μmol.

[0034] The concentration of lovastatin in the fermentation broth was determined by the same method as in Example 1. The yield of lovastatin produced by Monascus in this example was 6.57 µg / mL.

Example Embodiment

[0035] Example 3:

[0036] This example provides a method for culturing a high-yield lovastatin of Monascus. The specific culturing method is the same as that in Example 1, except that the amount of salicylic acid per liter of PDB liquid medium is 100 μmol.

[0037] The concentration of lovastatin in the fermentation broth was determined using the same method as in Example 1. The yield of lovastatin produced by Monascus in this example was 7.519 µg / mL.

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Abstract

The invention discloses a Monascus culture method capable of achieving high-yield lovastatin. The Monascus culture method comprises: (1) diluting and oscillating an activated Monascus strain to obtaina single spore suspension, inoculating the single spore suspension into a PDB liquid culture medium, and culturing for 12 days at a temperature of 30 DEG C to obtain a Monascus suspension; and (2) adding salicylic acid or methyl jasmonate to a PDB liquid culture medium, inoculating the Monascus suspension into the PDB liquid culture medium, and carrying out shaker culture for 12 days at a temperature of 30 DEG C under a rotation speed of 180 r/min. According to the present invention, salicylic acid or methyl jasmonate is added to the specific culture medium, and the Monascus is cultured underthe specific conditions, such that the biomass of the Monascus is effectively increased by promoting the growth of Monascus hyphae or regulating the metabolic process of Monascus so as to effectivelyimprove the Monascus metabolism and the yield of lovastatin; and the method has characteristics of high efficiency, short time consumption, simple operation, low cost and high promotion and application value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for cultivating high-yield lovastatin by Monascus. Background technique [0002] Currently, there are four main types of lipid-lowering drugs: resins, niacin, fibrates, and statins, among which statins represented by lovastatin are currently the most important class of lipid-lowering drugs. Lovastatin is a competitive inhibitor of the rate-limiting enzyme hydroxymethylglutaryl coenzyme A (HMG-CoA) reductase in endogenous cholesterol synthesis. It has been clinically verified that lovastatin is highly effective, safe and low-toxic A good drug for the treatment of hyperlipidemia. Since U.S. Merck Company developed the lovastatin series and launched a new generation of cholesterol-lowering drugs in 1987, lovastatin has been popular among patients as a new type of blood lipid regulator. [0003] At present, the production strains of lovastatin are mainly...

Claims

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Application Information

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IPC IPC(8): C12P17/06C12R1/645
CPCC12P17/06
Inventor 高梦祥张佳兰
Owner YANGTZE UNIVERSITY
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